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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001895                                                              20     299
NP_001091680                                                           20     299
NP_001091679                                                           20     299
XP_006713048                                                           20     299
XP_006713047                                                           20     299
XP_006713046                                                           20     299
XP_005264943                                                           20     299
NP_001124295                                                           17    4312
NP_001370                                                              17    4312
XP_006722744                                                           17    4312
NP_061963                                                              16    5631
NP_055986                                                              16    5631
NP_061903                                                              16    5631
NP_001257869                                                           16    5631
NP_060639                                                              16    5631
XP_005255257                                                           16    5631
XP_005255256                                                           16    5631
XP_005255255                                                           16    5631
XP_005255254                                                           16    5631
XP_005255252                                                           16    5631
XP_006719162                                                           16    5631
XP_005253462                                                           16    5631
XP_005248601                                                           16    5631
XP_006714716                                                           16    5631
NP_001004727                                                           16    5631
NP_004303                                                              16    7355
XP_006724714                                                           16    7355
NP_001265478                                                           15    9605
NP_002601                                                              15    9605
XP_006712657                                                           15    9605
NP_001073951                                                           15    9605

>NP_001895
          Length = 781

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  QGFSQSFAQ 9
          QGFSQSF Q
Sbjct: 68 QGFSQSFTQ 76



>NP_001091680
          Length = 781

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  QGFSQSFAQ 9
          QGFSQSF Q
Sbjct: 68 QGFSQSFTQ 76



>NP_001091679
          Length = 781

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  QGFSQSFAQ 9
          QGFSQSF Q
Sbjct: 68 QGFSQSFTQ 76



>XP_006713048
          Length = 720

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  QGFSQSFAQ 9
          QGFSQSF Q
Sbjct: 68 QGFSQSFTQ 76



>XP_006713047
          Length = 774

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  QGFSQSFAQ 9
          QGFSQSF Q
Sbjct: 61 QGFSQSFTQ 69



>XP_006713046
          Length = 774

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  QGFSQSFAQ 9
          QGFSQSF Q
Sbjct: 61 QGFSQSFTQ 69



>XP_005264943
          Length = 781

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  QGFSQSFAQ 9
          QGFSQSF Q
Sbjct: 68 QGFSQSFTQ 76



>NP_001124295
          Length = 1632

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GFSQSFAQ 9
           GFS SFA+
Sbjct: 494 GFSTSFAE 501



>NP_001370
          Length = 1616

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GFSQSFAQ 9
           GFS SFA+
Sbjct: 478 GFSTSFAE 485



>XP_006722744
          Length = 1495

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GFSQSFAQ 9
           GFS SFA+
Sbjct: 357 GFSTSFAE 364



>NP_061963
          Length = 1047

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QGFSQSFAQ 9
           +GFS SFA+
Sbjct: 124 RGFSFSFAE 132



>NP_055986
          Length = 1125

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   QGFSQSFAQ 9
           Q F QSF Q
Sbjct: 982 QAFGQSFLQ 990



>NP_061903
          Length = 1369

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QGFSQSFAQ 9
           +GFSQ F +
Sbjct: 173 EGFSQEFEE 181



>NP_001257869
          Length = 1111

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   QGFSQSFAQ 9
           Q F QSF Q
Sbjct: 968 QAFGQSFLQ 976



>NP_060639
          Length = 1747

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QGFSQSFAQ 9
           QG +QSF++
Sbjct: 184 QGLNQSFSE 192



>XP_005255257
          Length = 774

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   QGFSQSFAQ 9
           Q F QSF Q
Sbjct: 631 QAFGQSFLQ 639



>XP_005255256
          Length = 780

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   QGFSQSFAQ 9
           Q F QSF Q
Sbjct: 637 QAFGQSFLQ 645



>XP_005255255
          Length = 951

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   QGFSQSFAQ 9
           Q F QSF Q
Sbjct: 808 QAFGQSFLQ 816



>XP_005255254
          Length = 1051

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   QGFSQSFAQ 9
           Q F QSF Q
Sbjct: 908 QAFGQSFLQ 916



>XP_005255252
          Length = 1124

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   QGFSQSFAQ 9
           Q F QSF Q
Sbjct: 981 QAFGQSFLQ 989



>XP_006719162
          Length = 1676

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QGFSQSFAQ 9
           QG +QSF++
Sbjct: 184 QGLNQSFSE 192



>XP_005253462
          Length = 1747

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QGFSQSFAQ 9
           QG +QSF++
Sbjct: 184 QGLNQSFSE 192



>XP_005248601
          Length = 899

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QGFSQSFAQ 9
           +GFSQ F +
Sbjct: 173 EGFSQEFEE 181



>XP_006714716
          Length = 1318

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QGFSQSFAQ 9
           +GFSQ F +
Sbjct: 173 EGFSQEFEE 181



>NP_001004727
          Length = 303

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GFSQSFAQ 9
           GF  SFAQ
Sbjct: 144 GFMHSFAQ 151



>NP_004303
          Length = 388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FSQSFA 8
           FSQSFA
Sbjct: 265 FSQSFA 270



>XP_006724714
          Length = 343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FSQSFA 8
           FSQSFA
Sbjct: 220 FSQSFA 225



>NP_001265478
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  GFSQSFA 8
          GFS+SF+
Sbjct: 23 GFSRSFS 29



>NP_002601
          Length = 436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  GFSQSFA 8
          GFS+SF+
Sbjct: 23 GFSRSFS 29



>XP_006712657
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  GFSQSFA 8
          GFS+SF+
Sbjct: 23 GFSRSFS 29



>NP_001073951
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QGFSQSFA 8
           QGF  SFA
Sbjct: 288 QGFRGSFA 295



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.123    0.334 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 447,750
Number of extensions: 721
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)