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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     509
NP_001075109                                                           20     509
NP_982272                                                              17    4312
NP_001177387                                                           17    4312
NP_001180465                                                           17    4312
NP_001160135                                                           16    5631
NP_055855                                                              16    7355
XP_006712426                                                           16    7355
XP_006712425                                                           16    7355
XP_006712424                                                           16    7355
NP_004203                                                              16    7355
NP_996809                                                              15    9605
NP_065184                                                              15    9605

>NP_008835
          Length = 4128

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QFVFESLVI 9
            QFVFE+LVI
Sbjct: 1098 QFVFEALVI 1106



>NP_001075109
          Length = 4097

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QFVFESLVI 9
            QFVFE+LVI
Sbjct: 1098 QFVFEALVI 1106



>NP_982272
          Length = 2099

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QFVFESLV 8
           QF FES+V
Sbjct: 818 QFAFESVV 825



>NP_001177387
          Length = 1999

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QFVFESLV 8
           QF FES+V
Sbjct: 750 QFAFESVV 757



>NP_001180465
          Length = 2031

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QFVFESLV 8
           QF FES+V
Sbjct: 750 QFAFESVV 757



>NP_001160135
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FVFESLV 8
           FVFESL+
Sbjct: 124 FVFESLL 130



>NP_055855
          Length = 2098

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QFVFESLV 8
            Q VFESL+
Sbjct: 1484 QSVFESLI 1491



>XP_006712426
          Length = 1237

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QFVFESLV 8
           Q VFESL+
Sbjct: 790 QSVFESLI 797



>XP_006712425
          Length = 1349

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QFVFESLV 8
           Q VFESL+
Sbjct: 790 QSVFESLI 797



>XP_006712424
          Length = 1376

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QFVFESLV 8
           Q VFESL+
Sbjct: 790 QSVFESLI 797



>NP_004203
          Length = 658

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   QFVFESLVI 9
           QFVF  LVI
Sbjct: 209 QFVFGILVI 217



>NP_996809
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   QFVFESL 7
           QFVFE +
Sbjct: 363 QFVFEEI 369



>NP_065184
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   QFVFESL 7
           QFVFE +
Sbjct: 397 QFVFEEI 403



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.344    0.155    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 326,951
Number of extensions: 468
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)