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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              20     509
XP_006722001                                                           17    2528
XP_006722000                                                           17    2528
XP_006721999                                                           17    2528
XP_006721998                                                           17    2528
XP_006721997                                                           17    2528
XP_006721996                                                           17    2528
XP_006721995                                                           17    2528
NP_057423                                                              17    2528
NP_473447                                                              16    5631
NP_059431                                                              16    7355
NP_004219                                                              16    7355
XP_006723536                                                           16    7355
XP_006723535                                                           16    7355
NP_004932                                                              15    9605
NP_001077422                                                           15    9605
NP_071348                                                              15    9605
NP_001164209                                                           15    9605
XP_005257171                                                           15    9605
XP_005257170                                                           15    9605
XP_006712132                                                           15    9605
XP_006712130                                                           15    9605
XP_006712129                                                           15    9605
XP_006712128                                                           15    9605
XP_006712127                                                           15    9605
XP_006712126                                                           15    9605
XP_006712125                                                           15    9605
XP_006712124                                                           15    9605
NP_659486                                                              15    9605
NP_056047                                                              15    9605
NP_001163887                                                           15    9605

>NP_115927
          Length = 1321

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QEGSKTLVL 9
           +EGSKTLVL
Sbjct: 830 EEGSKTLVL 838



>XP_006722001
          Length = 392

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   EGSKTLV 8
           EGSKTLV
Sbjct: 318 EGSKTLV 324



>XP_006722000
          Length = 456

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   EGSKTLV 8
           EGSKTLV
Sbjct: 382 EGSKTLV 388



>XP_006721999
          Length = 493

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   EGSKTLV 8
           EGSKTLV
Sbjct: 419 EGSKTLV 425



>XP_006721998
          Length = 493

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   EGSKTLV 8
           EGSKTLV
Sbjct: 419 EGSKTLV 425



>XP_006721997
          Length = 493

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   EGSKTLV 8
           EGSKTLV
Sbjct: 419 EGSKTLV 425



>XP_006721996
          Length = 511

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   EGSKTLV 8
           EGSKTLV
Sbjct: 437 EGSKTLV 443



>XP_006721995
          Length = 662

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   EGSKTLV 8
           EGSKTLV
Sbjct: 588 EGSKTLV 594



>NP_057423
          Length = 456

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   EGSKTLV 8
           EGSKTLV
Sbjct: 382 EGSKTLV 388



>NP_473447
          Length = 309

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  EGSKTLV 8
          EG+KTLV
Sbjct: 5  EGNKTLV 11



>NP_059431
          Length = 400

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  EGSKTL 7
          EGSKTL
Sbjct: 54 EGSKTL 59



>NP_004219
          Length = 399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  EGSKTL 7
          EGSKTL
Sbjct: 54 EGSKTL 59



>XP_006723536
          Length = 394

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  EGSKTL 7
          EGSKTL
Sbjct: 76 EGSKTL 81



>XP_006723535
          Length = 421

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  EGSKTL 7
          EGSKTL
Sbjct: 76 EGSKTL 81



>NP_004932
          Length = 1220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    QEGSKTLV 8
            QEG +TL+
Sbjct: 1121 QEGYRTLI 1128



>NP_001077422
          Length = 1953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QEGSKTLVL 9
            +EG  TLVL
Sbjct: 1924 KEGEDTLVL 1932



>NP_071348
          Length = 1953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QEGSKTLVL 9
            +EG  TLVL
Sbjct: 1924 KEGEDTLVL 1932



>NP_001164209
          Length = 1182

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    QEGSKTL 7
            Q+G+KTL
Sbjct: 996  QQGTKTL 1002



>XP_005257171
          Length = 1181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    QEGSKTLV 8
            QEG +TL+
Sbjct: 1121 QEGYRTLI 1128



>XP_005257170
          Length = 1218

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    QEGSKTLV 8
            QEG +TL+
Sbjct: 1119 QEGYRTLI 1126



>XP_006712132
          Length = 1136

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QEGSKTLVL 9
            +EG  TLVL
Sbjct: 1107 KEGEDTLVL 1115



>XP_006712130
          Length = 1831

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QEGSKTLVL 9
            +EG  TLVL
Sbjct: 1802 KEGEDTLVL 1810



>XP_006712129
          Length = 1832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QEGSKTLVL 9
            +EG  TLVL
Sbjct: 1803 KEGEDTLVL 1811



>XP_006712128
          Length = 1879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QEGSKTLVL 9
            +EG  TLVL
Sbjct: 1850 KEGEDTLVL 1858



>XP_006712127
          Length = 1909

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QEGSKTLVL 9
            +EG  TLVL
Sbjct: 1880 KEGEDTLVL 1888



>XP_006712126
          Length = 1913

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QEGSKTLVL 9
            +EG  TLVL
Sbjct: 1884 KEGEDTLVL 1892



>XP_006712125
          Length = 1952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QEGSKTLVL 9
            +EG  TLVL
Sbjct: 1923 KEGEDTLVL 1931



>XP_006712124
          Length = 1953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QEGSKTLVL 9
            +EG  TLVL
Sbjct: 1924 KEGEDTLVL 1932



>NP_659486
          Length = 318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QEGSKTLVL 9
           +EG K LVL
Sbjct: 134 REGKKLLVL 142



>NP_056047
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QEGSKTL 7
           Q+G+KTL
Sbjct: 693 QQGTKTL 699



>NP_001163887
          Length = 1221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  EGSKTLV 8
          EGSKT++
Sbjct: 44 EGSKTIL 50



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.124    0.298 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 591,029
Number of extensions: 858
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)