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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              21     175
NP_005913                                                              21     175
XP_005267046                                                           21     175
XP_005267045                                                           21     175
NP_068817                                                              18    1935
NP_001239160                                                           18    1935
XP_005271911                                                           18    1935
XP_006714586                                                           18    1935
NP_059129                                                              17    3301
NP_001209                                                              17    4312
NP_996808                                                              17    4312
NP_852114                                                              16    5631
NP_001177768                                                           16    5631
NP_000891                                                              16    5631
NP_005946                                                              16    5631
NP_002284                                                              16    7355
NP_000737                                                              15    9605
NP_001177384                                                           15    9605
NP_689418                                                              15    9605
NP_444252                                                              15    9605
NP_683709                                                              15    9605
NP_647536                                                              15    9605
NP_998765                                                              15    9605
NP_002619                                                              15    9605
XP_005276529                                                           15    9605
XP_006725626                                                           15    9605
XP_005254807                                                           15    9605
XP_005254329                                                           15    9605

>NP_006715
          Length = 1558

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QEAMIQGYN 9
            +EAMIQGYN
Sbjct: 1033 REAMIQGYN 1041



>NP_005913
          Length = 1608

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QEAMIQGYN 9
            +EAMIQGYN
Sbjct: 1033 REAMIQGYN 1041



>XP_005267046
          Length = 1554

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QEAMIQGYN 9
            +EAMIQGYN
Sbjct: 1033 REAMIQGYN 1041



>XP_005267045
          Length = 1604

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QEAMIQGYN 9
            +EAMIQGYN
Sbjct: 1033 REAMIQGYN 1041



>NP_068817
          Length = 1093

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  QEAMIQGYN 9
          QE++ QGYN
Sbjct: 43 QESVSQGYN 51



>NP_001239160
          Length = 613

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  QEAMIQGYN 9
          QE++ QGYN
Sbjct: 43 QESVSQGYN 51



>XP_005271911
          Length = 613

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  QEAMIQGYN 9
          QE++ QGYN
Sbjct: 43 QESVSQGYN 51



>XP_006714586
          Length = 1035

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  QEAMIQGYN 9
          QE++ QGYN
Sbjct: 43 QESVSQGYN 51



>NP_059129
          Length = 1616

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QEAMIQGY 8
            +EAMIQ Y
Sbjct: 1233 EEAMIQSY 1240



>NP_001209
          Length = 354

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QEAMIQGYN 9
           QEA + G+N
Sbjct: 198 QEAFVPGFN 206



>NP_996808
          Length = 343

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QEAMIQGYN 9
           QEA + G+N
Sbjct: 198 QEAFVPGFN 206



>NP_852114
          Length = 205

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  EAMIQGYN 9
          E+M +GYN
Sbjct: 39 ESMYKGYN 46



>NP_001177768
          Length = 128

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   AMIQGYN 9
           AM+ GYN
Sbjct: 108 AMVYGYN 114



>NP_000891
          Length = 103

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  AMIQGYN 9
          AM+ GYN
Sbjct: 83 AMVYGYN 89



>NP_005946
          Length = 753

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  EAMIQGY 8
          EAM QGY
Sbjct: 87 EAMSQGY 93



>NP_002284
          Length = 1609

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    QEAMIQGYN 9
            QEA I  YN
Sbjct: 1568 QEAAIMDYN 1576



>NP_000737
          Length = 502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QEAMIQGY 8
           QEA I GY
Sbjct: 183 QEADISGY 190



>NP_001177384
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QEAMIQGY 8
           QEA I GY
Sbjct: 212 QEADISGY 219



>NP_689418
          Length = 839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   AMIQGY 8
           +MIQGY
Sbjct: 829 SMIQGY 834



>NP_444252
          Length = 140

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  QEAMIQGY 8
          QEA I GY
Sbjct: 18 QEAAIVGY 25



>NP_683709
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1 QEAMIQGY 8
         QEA I GY
Sbjct: 2 QEADISGY 9



>NP_647536
          Length = 412

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QEAMIQGY 8
           QEA I GY
Sbjct: 93  QEADISGY 100



>NP_998765
          Length = 849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QEAMIQGYN 9
           QEA+ QG N
Sbjct: 483 QEAVRQGQN 491



>NP_002619
          Length = 140

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  QEAMIQGY 8
          QEA I GY
Sbjct: 18 QEAAIVGY 25



>XP_005276529
          Length = 412

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QEAMIQGY 8
           QEA I GY
Sbjct: 93  QEADISGY 100



>XP_006725626
          Length = 290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QEAMIQGY 8
           QEA I GY
Sbjct: 93  QEADISGY 100



>XP_005254807
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1 QEAMIQGY 8
         QEA I GY
Sbjct: 2 QEADISGY 9



>XP_005254329
          Length = 481

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QEAMIQGYN 9
           QEA+ QG N
Sbjct: 115 QEAVRQGQN 123



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.126    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 427,023
Number of extensions: 758
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)