Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     299
NP_001075109                                                           20     299
NP_000954                                                              18    1935
NP_653252                                                              17    4312
XP_005251225                                                           17    4312
XP_006716483                                                           17    4312
XP_005251224                                                           17    4312
NP_001161842                                                           16    5631
NP_055595                                                              16    5631
NP_000669                                                              16    5631
XP_006715348                                                           16    5631
XP_005249560                                                           16    5631
NP_067640                                                              16    7355
NP_001171890                                                           16    7355
NP_001171891                                                           16    7355
NP_055883                                                              16    7355
NP_001035242                                                           16    7355
NP_001165908                                                           16    7355
NP_001165909                                                           16    7355
NP_005018                                                              16    7355
XP_005247748                                                           16    7355
XP_005247747                                                           16    7355
NP_006840                                                              15    9605
>NP_008835
          Length = 4128

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QAYPALMVE 9
            QAYPAL+VE
Sbjct: 3440 QAYPALVVE 3448
>NP_001075109
          Length = 4097

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QAYPALMVE 9
            QAYPAL+VE
Sbjct: 3440 QAYPALVVE 3448
>NP_000954
          Length = 604

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QAYPALMVE 9
           + YPAL+VE
Sbjct: 488 ELYPALLVE 496
>NP_653252
          Length = 1463

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    YPALMVE 9
            +PALMVE
Sbjct: 1197 WPALMVE 1203
>XP_005251225
          Length = 848

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   YPALMVE 9
           +PALMVE
Sbjct: 582 WPALMVE 588
>XP_006716483
          Length = 1367

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    YPALMVE 9
            +PALMVE
Sbjct: 1101 WPALMVE 1107
>XP_005251224
          Length = 1439

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    YPALMVE 9
            +PALMVE
Sbjct: 1173 WPALMVE 1179
>NP_001161842
          Length = 1782

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QAYPALM 7
           QAYP+L+
Sbjct: 661 QAYPSLL 667
>NP_055595
          Length = 1698

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QAYPALM 7
           QAYP+L+
Sbjct: 577 QAYPSLL 583
>NP_000669
          Length = 572

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YPALMVE 9
           YPA+M E
Sbjct: 204 YPAIMTE 210
>XP_006715348
          Length = 1734

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QAYPALM 7
           QAYP+L+
Sbjct: 609 QAYPSLL 615
>XP_005249560
          Length = 1750

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QAYPALM 7
           QAYP+L+
Sbjct: 629 QAYPSLL 635
>NP_067640
          Length = 589

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QAYPALMV 8
           Q YPAL V
Sbjct: 430 QGYPALHV 437
>NP_001171890
          Length = 742

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YPALMVE 9
           YPA M+E
Sbjct: 181 YPAFMME 187
>NP_001171891
          Length = 359

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YPALMVE 9
           YPA M+E
Sbjct: 181 YPAFMME 187
>NP_055883
          Length = 708

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YPALMVE 9
           YPA M+E
Sbjct: 147 YPAFMME 153
>NP_001035242
          Length = 325

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YPALMVE 9
           YPA M+E
Sbjct: 147 YPAFMME 153
>NP_001165908
          Length = 784

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YPALMVE 9
           YPA M+E
Sbjct: 223 YPAFMME 229
>NP_001165909
          Length = 401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YPALMVE 9
           YPA M+E
Sbjct: 223 YPAFMME 229
>NP_005018
          Length = 728

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   YPALMVE 9
           +PAL+VE
Sbjct: 279 FPALLVE 285
>XP_005247748
          Length = 474

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QAYPALMV 8
           Q YPAL V
Sbjct: 315 QGYPALHV 322
>XP_005247747
          Length = 620

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QAYPALMV 8
           Q YPAL V
Sbjct: 461 QGYPALHV 468
>NP_006840
          Length = 525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  YPALMVE 9
          +PAL+VE
Sbjct: 59 HPALLVE 65
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.319    0.128    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 432,670
Number of extensions: 327
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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