Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           22     134
NP_004561                                                              22     134
NP_001275703                                                           22     134
XP_005253450                                                           22     134
XP_006719151                                                           22     134
NP_001010938                                                           18    1935
NP_005772                                                              18    1935
NP_005392                                                              18    1935
XP_005269332                                                           18    1935
XP_005269331                                                           18    1935
XP_005269330                                                           18    1935
XP_005269329                                                           18    1935
XP_005269328                                                           18    1935
XP_005269327                                                           18    1935
XP_006713530                                                           18    1935
XP_006713529                                                           18    1935
XP_005269326                                                           18    1935
XP_006713528                                                           18    1935
XP_006713527                                                           18    1935
XP_005269325                                                           18    1935
XP_006713526                                                           18    1935
XP_006713525                                                           18    1935
XP_006713524                                                           18    1935
XP_006713523                                                           18    1935
XP_005271739                                                           17    3301
NP_003469                                                              17    3301
NP_997191                                                              17    4312
NP_055221                                                              17    4312
NP_001152956                                                           17    4312
XP_005258568                                                           17    4312
XP_006711682                                                           17    4312
NP_789781                                                              17    4312
NP_078898                                                              16    5631
NP_001268405                                                           16    5631
NP_849156                                                              16    5631
NP_001245319                                                           16    5631
XP_006726780                                                           16    5631
XP_006721690                                                           16    5631
XP_006718832                                                           16    5631
XP_006712814                                                           16    5631
XP_006712813                                                           16    5631
XP_006710091                                                           16    5631
NP_001051                                                              16    7355
NP_963998                                                              16    7355
NP_076985                                                              16    7355
NP_004127                                                              15    9605
NP_776195                                                              15    9605
NP_001182143                                                           15    9605
NP_001123990                                                           15    9605
NP_060841                                                              15    9605
XP_006716429                                                           15    9605
XP_006716427                                                           15    9605
XP_006716426                                                           15    9605
XP_006716425                                                           15    9605
XP_006716424                                                           15    9605
XP_006716423                                                           15    9605
XP_006716422                                                           15    9605
XP_006716421                                                           15    9605
XP_005272029                                                           15    9605
NP_536356                                                              15    9605
>NP_001275701
          Length = 1486

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QAVMFEWNL 9
           QAV FEWNL
Sbjct: 803 QAVKFEWNL 811
>NP_004561
          Length = 1445

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QAVMFEWNL 9
           QAV FEWNL
Sbjct: 762 QAVKFEWNL 770
>NP_001275703
          Length = 1264

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QAVMFEWNL 9
           QAV FEWNL
Sbjct: 581 QAVKFEWNL 589
>XP_005253450
          Length = 1486

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QAVMFEWNL 9
           QAV FEWNL
Sbjct: 803 QAVKFEWNL 811
>XP_006719151
          Length = 1486

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QAVMFEWNL 9
           QAV FEWNL
Sbjct: 803 QAVKFEWNL 811
>NP_001010938
          Length = 1086

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MFEWNL 9
            MF+WNL
Sbjct: 1063 MFDWNL 1068
>NP_005772
          Length = 1038

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MFEWNL 9
            MF+WNL
Sbjct: 1015 MFDWNL 1020
>NP_005392
          Length = 1187

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QAVMFEWN 8
           QAV++ WN
Sbjct: 248 QAVIYRWN 255
>XP_005269332
          Length = 741

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MFEWNL 9
           MF+WNL
Sbjct: 718 MFDWNL 723
>XP_005269331
          Length = 816

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MFEWNL 9
           MF+WNL
Sbjct: 793 MFDWNL 798
>XP_005269330
          Length = 979

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MFEWNL 9
           MF+WNL
Sbjct: 956 MFDWNL 961
>XP_005269329
          Length = 1040

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MFEWNL 9
            MF+WNL
Sbjct: 1017 MFDWNL 1022
>XP_005269328
          Length = 1055

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MFEWNL 9
            MF+WNL
Sbjct: 1032 MFDWNL 1037
>XP_005269327
          Length = 1055

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MFEWNL 9
            MF+WNL
Sbjct: 1032 MFDWNL 1037
>XP_006713530
          Length = 1063

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MFEWNL 9
            MF+WNL
Sbjct: 1040 MFDWNL 1045
>XP_006713529
          Length = 1085

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MFEWNL 9
            MF+WNL
Sbjct: 1063 MFDWNL 1068
>XP_005269326
          Length = 1087

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MFEWNL 9
            MF+WNL
Sbjct: 1064 MFDWNL 1069
>XP_006713528
          Length = 1101

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MFEWNL 9
            MF+WNL
Sbjct: 1078 MFDWNL 1083
>XP_006713527
          Length = 1115

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MFEWNL 9
            MF+WNL
Sbjct: 1093 MFDWNL 1098
>XP_005269325
          Length = 1118

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MFEWNL 9
            MF+WNL
Sbjct: 1095 MFDWNL 1100
>XP_006713526
          Length = 1118

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MFEWNL 9
            MF+WNL
Sbjct: 1095 MFDWNL 1100
>XP_006713525
          Length = 1119

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MFEWNL 9
            MF+WNL
Sbjct: 1096 MFDWNL 1101
>XP_006713524
          Length = 1131

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MFEWNL 9
            MF+WNL
Sbjct: 1108 MFDWNL 1113
>XP_006713523
          Length = 1133

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    MFEWNL 9
            MF+WNL
Sbjct: 1110 MFDWNL 1115
>XP_005271739
          Length = 721

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AVMFEWN 8
           AV+F WN
Sbjct: 542 AVLFAWN 548
>NP_003469
          Length = 780

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AVMFEWN 8
           AV+F WN
Sbjct: 601 AVLFAWN 607
>NP_997191
          Length = 1888

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VMFEWNL 9
           +MFEW++
Sbjct: 167 MMFEWSI 173
>NP_055221
          Length = 557

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   AVMFEW 7
           AV+FEW
Sbjct: 360 AVVFEW 365
>NP_001152956
          Length = 1736

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VMFEWNL 9
           +MFEW++
Sbjct: 167 MMFEWSI 173
>XP_005258568
          Length = 1029

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   VMFEW 7
           VMFEW
Sbjct: 223 VMFEW 227
>XP_006711682
          Length = 1739

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3  VMFEWNL 9
          +MFEW++
Sbjct: 18 MMFEWSI 24
>NP_789781
          Length = 1048

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   VMFEW 7
           VMFEW
Sbjct: 223 VMFEW 227
>NP_078898
          Length = 847

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1   QAVMFEWN 8
           Q   FEWN
Sbjct: 814 QISQFEWN 821
>NP_001268405
          Length = 804

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1   QAVMFEWN 8
           Q   FEWN
Sbjct: 771 QISQFEWN 778
>NP_849156
          Length = 374

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 4   MFEWN 8
           MF+WN
Sbjct: 160 MFQWN 164
>NP_001245319
          Length = 382

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 4   MFEWN 8
           MF+WN
Sbjct: 168 MFQWN 172
>XP_006726780
          Length = 293

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  QAVMFEWNL 9
          QAV++ W L
Sbjct: 70 QAVLYIWEL 78
>XP_006721690
          Length = 293

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  QAVMFEWNL 9
          QAV++ W L
Sbjct: 70 QAVLYIWEL 78
>XP_006718832
          Length = 230

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 4  MFEWN 8
          MF+WN
Sbjct: 16 MFQWN 20
>XP_006712814
          Length = 527

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1   QAVMFEWN 8
           Q   FEWN
Sbjct: 494 QISQFEWN 501
>XP_006712813
          Length = 824

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1   QAVMFEWN 8
           Q   FEWN
Sbjct: 791 QISQFEWN 798
>XP_006710091
          Length = 293

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  QAVMFEWNL 9
          QAV++ W L
Sbjct: 70 QAVLYIWEL 78
>NP_001051
          Length = 343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2  AVMFEWN 8
          A +FEW+
Sbjct: 90 AALFEWH 96
>NP_963998
          Length = 407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2  AVMFEWN 8
          A +FEW+
Sbjct: 90 AALFEWH 96
>NP_076985
          Length = 1104

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   VMFEWNL 9
           V+F WN+
Sbjct: 736 VVFSWNV 742
>NP_004127
          Length = 963

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   AVMFEWNL 9
           +V+F W+L
Sbjct: 706 SVLFPWDL 713
>NP_776195
          Length = 236

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   VMFEWNL 9
           VMF W++
Sbjct: 190 VMFAWSI 196
>NP_001182143
          Length = 193

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   VMFEWNL 9
           VMF W++
Sbjct: 147 VMFAWSI 153
>NP_001123990
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QAVMFEW 7
          QAV+ EW
Sbjct: 58 QAVLQEW 64
>NP_060841
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QAVMFEW 7
          QAV+ EW
Sbjct: 58 QAVLQEW 64
>XP_006716429
          Length = 297

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QAVMFEW 7
          QAV+ EW
Sbjct: 58 QAVLQEW 64
>XP_006716427
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QAVMFEW 7
          QAV+ EW
Sbjct: 58 QAVLQEW 64
>XP_006716426
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QAVMFEW 7
          QAV+ EW
Sbjct: 58 QAVLQEW 64
>XP_006716425
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QAVMFEW 7
          QAV+ EW
Sbjct: 58 QAVLQEW 64
>XP_006716424
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QAVMFEW 7
          QAV+ EW
Sbjct: 58 QAVLQEW 64
>XP_006716423
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QAVMFEW 7
          QAV+ EW
Sbjct: 58 QAVLQEW 64
>XP_006716422
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QAVMFEW 7
          QAV+ EW
Sbjct: 58 QAVLQEW 64
>XP_006716421
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QAVMFEW 7
          QAV+ EW
Sbjct: 58 QAVLQEW 64
>XP_005272029
          Length = 213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   QAVMFEW 7
           QAV F W
Sbjct: 185 QAVSFTW 191
>NP_536356
          Length = 377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 4  MFEWNL 9
          +FEW L
Sbjct: 93 LFEWEL 98
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.131    0.483 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 556,834
Number of extensions: 364
Number of successful extensions: 60
Number of sequences better than 10000.0: 60
Number of HSP's gapped: 60
Number of HSP's successfully gapped: 60
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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