Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              19     665
NP_653080                                                              19     665
XP_005267292                                                           19     665
XP_005267291                                                           19     665
XP_005267290                                                           19     665
NP_001131140                                                           18    1482
NP_036247                                                              18    1482
NP_001131139                                                           18    1482
NP_036223                                                              17    4312
NP_036450                                                              16    5631
XP_005248027                                                           16    5631
NP_056023                                                              16    7355
NP_001274513                                                           16    7355
NP_001190985                                                           16    7355
NP_001077094                                                           16    7355
NP_001274514                                                           16    7355
NP_612142                                                              16    7355
XP_005258153                                                           16    7355
XP_005258152                                                           16    7355
XP_005258151                                                           16    7355
XP_005258150                                                           16    7355
XP_005258149                                                           16    7355
XP_006712318                                                           16    7355
XP_006712317                                                           16    7355
XP_005246341                                                           16    7355
NP_060530                                                              15    9605
NP_001164163                                                           15    9605
NP_995586                                                              15    9605
NP_001164164                                                           15    9605
NP_149096                                                              15    9605
>NP_005091
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QARSDISKD 9
            QA SDISKD
Sbjct: 1625 QAHSDISKD 1633
>NP_653080
          Length = 1684

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QARSDISKD 9
            QA SDISKD
Sbjct: 1527 QAHSDISKD 1535
>XP_005267292
          Length = 1677

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QARSDISKD 9
            QA SDISKD
Sbjct: 1520 QAHSDISKD 1528
>XP_005267291
          Length = 1684

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QARSDISKD 9
            QA SDISKD
Sbjct: 1527 QAHSDISKD 1535
>XP_005267290
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QARSDISKD 9
            QA SDISKD
Sbjct: 1625 QAHSDISKD 1633
>NP_001131140
          Length = 1982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   QARSDISKD 9
           +A+SD+SKD
Sbjct: 180 RAKSDVSKD 188
>NP_036247
          Length = 1982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   QARSDISKD 9
           +A+SD+SKD
Sbjct: 180 RAKSDVSKD 188
>NP_001131139
          Length = 1982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   QARSDISKD 9
           +A+SD+SKD
Sbjct: 180 RAKSDVSKD 188
>NP_036223
          Length = 502

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   QARSDISK 8
           QARS ISK
Sbjct: 428 QARSQISK 435
>NP_036450
          Length = 739

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QARSDISKD 9
           Q RS+++KD
Sbjct: 486 QKRSEVAKD 494
>XP_005248027
          Length = 434

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QARSDISKD 9
           Q RS+++KD
Sbjct: 181 QKRSEVAKD 189
>NP_056023
          Length = 2062

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  RSDISKD 9
          RSD+SK+
Sbjct: 45 RSDVSKE 51
>NP_001274513
          Length = 309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QARSDISKD 9
           QAR  +SKD
Sbjct: 203 QARKALSKD 211
>NP_001190985
          Length = 2039

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  RSDISKD 9
          RSD+SK+
Sbjct: 45 RSDVSKE 51
>NP_001077094
          Length = 2039

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  RSDISKD 9
          RSD+SK+
Sbjct: 45 RSDVSKE 51
>NP_001274514
          Length = 208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QARSDISKD 9
           QAR  +SKD
Sbjct: 102 QARKALSKD 110
>NP_612142
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QARSDISKD 9
           QAR  +SKD
Sbjct: 220 QARKALSKD 228
>XP_005258153
          Length = 2037

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  RSDISKD 9
          RSD+SK+
Sbjct: 45 RSDVSKE 51
>XP_005258152
          Length = 2060

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  RSDISKD 9
          RSD+SK+
Sbjct: 45 RSDVSKE 51
>XP_005258151
          Length = 2062

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  RSDISKD 9
          RSD+SK+
Sbjct: 45 RSDVSKE 51
>XP_005258150
          Length = 2062

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  RSDISKD 9
          RSD+SK+
Sbjct: 45 RSDVSKE 51
>XP_005258149
          Length = 2062

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  RSDISKD 9
          RSD+SK+
Sbjct: 45 RSDVSKE 51
>XP_006712318
          Length = 309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QARSDISKD 9
           QAR  +SKD
Sbjct: 203 QARKALSKD 211
>XP_006712317
          Length = 309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QARSDISKD 9
           QAR  +SKD
Sbjct: 203 QARKALSKD 211
>XP_005246341
          Length = 329

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QARSDISKD 9
           QAR  +SKD
Sbjct: 223 QARKALSKD 231
>NP_060530
          Length = 1012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ARSDISK 8
           AR DISK
Sbjct: 719 ARDDISK 725
>NP_001164163
          Length = 839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RSDISKD 9
           +SDI+KD
Sbjct: 577 KSDITKD 583
>NP_995586
          Length = 641

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RSDISKD 9
           +SDI+KD
Sbjct: 441 KSDITKD 447
>NP_001164164
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RSDISKD 9
           +SDI+KD
Sbjct: 577 KSDITKD 583
>NP_149096
          Length = 199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QARSDISKD 9
           Q +SD+ KD
Sbjct: 161 QIKSDMEKD 169
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.301    0.115    0.276 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 425,688
Number of extensions: 641
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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