Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              19     665
NP_001035957                                                           19     665
XP_006721991                                                           19     665
XP_006721990                                                           19     665
XP_006721989                                                           19     665
XP_005258041                                                           19     665
XP_006721988                                                           19     665
XP_006721987                                                           19     665
XP_006721986                                                           19     665
XP_005258040                                                           19     665
XP_006721985                                                           19     665
XP_005249326                                                           17    4312
XP_006715218                                                           17    4312
NP_068762                                                              17    4312
NP_005742                                                              16    5631
NP_671714                                                              16    5631
XP_006715890                                                           16    5631
XP_005250160                                                           16    5631
XP_005250159                                                           16    5631
NP_001277114                                                           16    7355
NP_068375                                                              16    7355
XP_005249641                                                           16    7355
NP_066939                                                              16    7355
NP_003280                                                              15    9605
NP_998839                                                              15    9605
NP_997320                                                              15    9605
XP_005253612                                                           15    9605
XP_005251629                                                           15    9605
XP_005251628                                                           15    9605
XP_005251627                                                           15    9605
XP_005251626                                                           15    9605
XP_005251625                                                           15    9605
XP_005251624                                                           15    9605
XP_005251623                                                           15    9605
>NP_000258
          Length = 2818

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QAQTKLEMA 9
           QAQTKLE+A
Sbjct: 682 QAQTKLEVA 690
>NP_001035957
          Length = 2839

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QAQTKLEMA 9
           QAQTKLE+A
Sbjct: 682 QAQTKLEVA 690
>XP_006721991
          Length = 1632

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QAQTKLEMA 9
           QAQTKLE+A
Sbjct: 692 QAQTKLEVA 700
>XP_006721990
          Length = 2823

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QAQTKLEMA 9
           QAQTKLE+A
Sbjct: 692 QAQTKLEVA 700
>XP_006721989
          Length = 2823

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QAQTKLEMA 9
           QAQTKLE+A
Sbjct: 692 QAQTKLEVA 700
>XP_005258041
          Length = 2836

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QAQTKLEMA 9
           QAQTKLE+A
Sbjct: 682 QAQTKLEVA 690
>XP_006721988
          Length = 2846

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QAQTKLEMA 9
           QAQTKLE+A
Sbjct: 692 QAQTKLEVA 700
>XP_006721987
          Length = 2849

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QAQTKLEMA 9
           QAQTKLE+A
Sbjct: 692 QAQTKLEVA 700
>XP_006721986
          Length = 2854

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QAQTKLEMA 9
           QAQTKLE+A
Sbjct: 679 QAQTKLEVA 687
>XP_005258040
          Length = 2857

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QAQTKLEMA 9
           QAQTKLE+A
Sbjct: 682 QAQTKLEVA 690
>XP_006721985
          Length = 2867

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QAQTKLEMA 9
           QAQTKLE+A
Sbjct: 692 QAQTKLEVA 700
>XP_005249326
          Length = 205

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QAQTKLEM 8
           Q QTKLE+
Sbjct: 137 QCQTKLEL 144
>XP_006715218
          Length = 218

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QAQTKLEM 8
           Q QTKLE+
Sbjct: 159 QCQTKLEL 166
>NP_068762
          Length = 227

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QAQTKLEM 8
           Q QTKLE+
Sbjct: 159 QCQTKLEL 166
>NP_005742
          Length = 3907

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AQTKLEM 8
            AQTKLE+
Sbjct: 3169 AQTKLEL 3175
>NP_671714
          Length = 3899

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AQTKLEM 8
            AQTKLE+
Sbjct: 3161 AQTKLEL 3167
>XP_006715890
          Length = 3860

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AQTKLEM 8
            AQTKLE+
Sbjct: 3122 AQTKLEL 3128
>XP_005250160
          Length = 3939

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AQTKLEM 8
            AQTKLE+
Sbjct: 3201 AQTKLEL 3207
>XP_005250159
          Length = 3952

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AQTKLEM 8
            AQTKLE+
Sbjct: 3214 AQTKLEL 3220
>NP_001277114
          Length = 931

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   QTKLEM 8
           QTKLEM
Sbjct: 348 QTKLEM 353
>NP_068375
          Length = 941

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   QTKLEM 8
           QTKLEM
Sbjct: 358 QTKLEM 363
>XP_005249641
          Length = 979

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QTKLEMA 9
           QT+LEMA
Sbjct: 577 QTELEMA 583
>NP_066939
          Length = 1119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QTKLEMA 9
           QT+LEMA
Sbjct: 577 QTELEMA 583
>NP_003280
          Length = 284

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  QAQTKLEMA 9
          +AQ KLE A
Sbjct: 66 EAQEKLEQA 74
>NP_998839
          Length = 284

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  QAQTKLEMA 9
          +AQ KLE A
Sbjct: 66 EAQEKLEQA 74
>NP_997320
          Length = 4471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    QAQTKL 6
            QAQTKL
Sbjct: 2128 QAQTKL 2133
>XP_005253612
          Length = 4589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    QAQTKL 6
            QAQTKL
Sbjct: 2246 QAQTKL 2251
>XP_005251629
          Length = 213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  QAQTKLEMA 9
          +AQ KLE A
Sbjct: 66 EAQEKLEQA 74
>XP_005251628
          Length = 213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  QAQTKLEMA 9
          +AQ KLE A
Sbjct: 66 EAQEKLEQA 74
>XP_005251627
          Length = 284

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  QAQTKLEMA 9
          +AQ KLE A
Sbjct: 66 EAQEKLEQA 74
>XP_005251626
          Length = 284

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  QAQTKLEMA 9
          +AQ KLE A
Sbjct: 66 EAQEKLEQA 74
>XP_005251625
          Length = 284

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  QAQTKLEMA 9
          +AQ KLE A
Sbjct: 66 EAQEKLEQA 74
>XP_005251624
          Length = 303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  QAQTKLEMA 9
          +AQ KLE A
Sbjct: 66 EAQEKLEQA 74
>XP_005251623
          Length = 303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  QAQTKLEMA 9
          +AQ KLE A
Sbjct: 66 EAQEKLEQA 74
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.106    0.238 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 347,929
Number of extensions: 535
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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