Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              20     509
XP_006716142                                                           20     509
XP_005250088                                                           20     509
XP_006716141                                                           20     509
XP_005250085                                                           20     509
XP_006716140                                                           20     509
XP_005250084                                                           20     509
XP_005250083                                                           20     509
XP_005250082                                                           20     509
NP_057636                                                              18    1935
NP_001269710                                                           18    1935
NP_001269709                                                           18    1935
NP_001269700                                                           18    1935
XP_006712635                                                           18    1935
XP_006712634                                                           18    1935
XP_006712633                                                           18    1935
XP_006712632                                                           18    1935
XP_005247077                                                           18    1935
XP_006712631                                                           18    1935
XP_006712630                                                           18    1935
NP_665803                                                              17    4312
NP_001275644                                                           16    5631
NP_001166094                                                           16    5631
NP_002490                                                              16    5631
NP_001323                                                              16    5631
XP_005254473                                                           16    5631
XP_005254472                                                           16    5631
XP_005254471                                                           16    5631
XP_005254470                                                           16    5631
XP_005254469                                                           16    5631
XP_005254468                                                           16    5631
XP_005254467                                                           16    5631
XP_005254465                                                           16    5631
XP_005248310                                                           16    5631
XP_005248309                                                           16    5631
XP_005248308                                                           16    5631
NP_001166095                                                           16    5631
NP_001714                                                              16    7355
NP_009171                                                              16    7355
XP_005249383                                                           16    7355
XP_005244919                                                           16    7355
NP_037483                                                              15    9605
NP_078966                                                              15    9605
NP_065684                                                              15    9605
NP_001193762                                                           15    9605
NP_874369                                                              15    9605
NP_006379                                                              15    9605
NP_874368                                                              15    9605
NP_001193856                                                           15    9605
NP_057363                                                              15    9605
XP_006718484                                                           15    9605
XP_006715067                                                           15    9605
>NP_733751
          Length = 4911

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QAMYYSTDT 9
            QAMY STDT
Sbjct: 3876 QAMYSSTDT 3884
>XP_006716142
          Length = 4927

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QAMYYSTDT 9
            QAMY STDT
Sbjct: 3893 QAMYSSTDT 3901
>XP_005250088
          Length = 4928

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QAMYYSTDT 9
            QAMY STDT
Sbjct: 3893 QAMYSSTDT 3901
>XP_006716141
          Length = 4959

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QAMYYSTDT 9
            QAMY STDT
Sbjct: 3893 QAMYSSTDT 3901
>XP_005250085
          Length = 4979

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QAMYYSTDT 9
            QAMY STDT
Sbjct: 3893 QAMYSSTDT 3901
>XP_006716140
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QAMYYSTDT 9
            QAMY STDT
Sbjct: 3893 QAMYSSTDT 3901
>XP_005250084
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QAMYYSTDT 9
            QAMY STDT
Sbjct: 3893 QAMYSSTDT 3901
>XP_005250083
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QAMYYSTDT 9
            QAMY STDT
Sbjct: 3892 QAMYSSTDT 3900
>XP_005250082
          Length = 4983

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QAMYYSTDT 9
            QAMY STDT
Sbjct: 3893 QAMYSSTDT 3901
>NP_057636
          Length = 941

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   YYSTDT 9
           YYSTDT
Sbjct: 646 YYSTDT 651
>NP_001269710
          Length = 702

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   YYSTDT 9
           YYSTDT
Sbjct: 407 YYSTDT 412
>NP_001269709
          Length = 720

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   YYSTDT 9
           YYSTDT
Sbjct: 505 YYSTDT 510
>NP_001269700
          Length = 1030

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   YYSTDT 9
           YYSTDT
Sbjct: 735 YYSTDT 740
>XP_006712635
          Length = 610

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   YYSTDT 9
           YYSTDT
Sbjct: 315 YYSTDT 320
>XP_006712634
          Length = 703

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   YYSTDT 9
           YYSTDT
Sbjct: 408 YYSTDT 413
>XP_006712633
          Length = 711

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   YYSTDT 9
           YYSTDT
Sbjct: 416 YYSTDT 421
>XP_006712632
          Length = 764

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   YYSTDT 9
           YYSTDT
Sbjct: 505 YYSTDT 510
>XP_005247077
          Length = 778

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   YYSTDT 9
           YYSTDT
Sbjct: 483 YYSTDT 488
>XP_006712631
          Length = 800

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   YYSTDT 9
           YYSTDT
Sbjct: 505 YYSTDT 510
>XP_006712630
          Length = 941

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   YYSTDT 9
           YYSTDT
Sbjct: 646 YYSTDT 651
>NP_665803
          Length = 1025

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QAMYYSTD 8
           +AMYYS +
Sbjct: 719 EAMYYSAE 726
>NP_001275644
          Length = 1134

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AMYYSTDT 9
           A+YYS+ T
Sbjct: 160 ALYYSSST 167
>NP_001166094
          Length = 1408

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MYYSTD 8
           +YYSTD
Sbjct: 992 IYYSTD 997
>NP_002490
          Length = 1461

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MYYSTD 8
           +YYSTD
Sbjct: 992 IYYSTD 997
>NP_001323
          Length = 1225

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AMYYSTDT 9
           A+YYS+ T
Sbjct: 251 ALYYSSST 258
>XP_005254473
          Length = 1408

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MYYSTD 8
           +YYSTD
Sbjct: 992 IYYSTD 997
>XP_005254472
          Length = 1428

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    MYYSTD 8
            +YYSTD
Sbjct: 1012 IYYSTD 1017
>XP_005254471
          Length = 1450

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MYYSTD 8
           +YYSTD
Sbjct: 992 IYYSTD 997
>XP_005254470
          Length = 1454

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    MYYSTD 8
            +YYSTD
Sbjct: 996  IYYSTD 1001
>XP_005254469
          Length = 1461

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MYYSTD 8
           +YYSTD
Sbjct: 992 IYYSTD 997
>XP_005254468
          Length = 1465

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    MYYSTD 8
            +YYSTD
Sbjct: 996  IYYSTD 1001
>XP_005254467
          Length = 1470

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    MYYSTD 8
            +YYSTD
Sbjct: 1012 IYYSTD 1017
>XP_005254465
          Length = 1481

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    MYYSTD 8
            +YYSTD
Sbjct: 1012 IYYSTD 1017
>XP_005248310
          Length = 1159

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AMYYSTDT 9
           A+YYS+ T
Sbjct: 160 ALYYSSST 167
>XP_005248309
          Length = 1236

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AMYYSTDT 9
           A+YYS+ T
Sbjct: 237 ALYYSSST 244
>XP_005248308
          Length = 1250

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AMYYSTDT 9
           A+YYS+ T
Sbjct: 251 ALYYSSST 258
>NP_001166095
          Length = 1450

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MYYSTD 8
           +YYSTD
Sbjct: 992 IYYSTD 997
>NP_001714
          Length = 2649

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    QAMYYS 6
            QA+YYS
Sbjct: 2299 QALYYS 2304
>NP_009171
          Length = 340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   QAMYYSTDT 9
           QAM Y  DT
Sbjct: 166 QAMGYEVDT 174
>XP_005249383
          Length = 3186

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    QAMYYS 6
            QA+YYS
Sbjct: 2836 QALYYS 2841
>XP_005244919
          Length = 210

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  QAMYYSTDT 9
          QAM Y  DT
Sbjct: 36 QAMGYEVDT 44
>NP_037483
          Length = 535

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QAMYYSTDT 9
           ++MY S DT
Sbjct: 329 ESMYTSVDT 337
>NP_078966
          Length = 14507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    AMYYSTDT 9
            AM++ST+T
Sbjct: 5415 AMHHSTNT 5422
>NP_065684
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3  MYYSTDT 9
          ++YSTD+
Sbjct: 20 LFYSTDS 26
>NP_001193762
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   QAMYYSTD 8
           + +YY TD
Sbjct: 308 KTLYYDTD 315
>NP_874369
          Length = 546

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   QAMYYSTD 8
           + +YY TD
Sbjct: 360 KTLYYDTD 367
>NP_006379
          Length = 513

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   QAMYYSTD 8
           + +YY TD
Sbjct: 327 KTLYYDTD 334
>NP_874368
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   QAMYYSTD 8
           + +YY TD
Sbjct: 275 KTLYYDTD 282
>NP_001193856
          Length = 4707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    QAMYYSTDT 9
            Q  YY TD+
Sbjct: 2630 QPYYYPTDS 2638
>NP_057363
          Length = 789

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   AMYYST 7
           AMYY T
Sbjct: 489 AMYYET 494
>XP_006718484
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   QAMYYSTD 8
           + +YY TD
Sbjct: 336 KTLYYDTD 343
>XP_006715067
          Length = 4707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    QAMYYSTDT 9
            Q  YY TD+
Sbjct: 2630 QPYYYPTDS 2638
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.114    0.315 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 500,358
Number of extensions: 705
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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