Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              20     299
NP_000421                                                              17    4312
NP_001121371                                                           17    4312
NP_001269092                                                           17    4312
NP_001121370                                                           17    4312
NP_000075                                                              17    4312
NP_005119                                                              17    4312
XP_006724131                                                           17    4312
NP_001274523                                                           16    5631
NP_001274522                                                           16    5631
NP_599030                                                              16    5631
NP_001279                                                              16    5631
NP_001273409                                                           16    5631
NP_055598                                                              16    5631
NP_001273408                                                           16    5631
XP_006725628                                                           16    5631
XP_006725627                                                           16    5631
XP_005257062                                                           16    5631
XP_006720851                                                           16    5631
XP_005254865                                                           16    5631
NP_001073936                                                           16    7355
NP_057195                                                              16    7355
XP_006726617                                                           16    7355
XP_006721056                                                           16    7355
XP_006720409                                                           16    7355
XP_006710039                                                           16    7355
NP_001180360                                                           15    9605
NP_057051                                                              15    9605
NP_036543                                                              15    9605
NP_001193727                                                           15    9605
NP_001193725                                                           15    9605
NP_001193728                                                           15    9605
NP_872271                                                              15    9605
NP_872270                                                              15    9605
NP_002645                                                              15    9605
NP_001265558                                                           15    9605
NP_689999                                                              15    9605
NP_001265559                                                           15    9605
NP_001248757                                                           15    9605
NP_001248756                                                           15    9605
NP_001073160                                                           15    9605
NP_115986                                                              15    9605
NP_001129465                                                           15    9605
NP_694943                                                              15    9605
NP_038477                                                              15    9605
NP_116242                                                              15    9605
XP_005258255                                                           15    9605
XP_005254502                                                           15    9605
XP_005254500                                                           15    9605
XP_006720633                                                           15    9605
XP_005268661                                                           15    9605
XP_005268660                                                           15    9605
XP_005268659                                                           15    9605
XP_005268657                                                           15    9605
XP_006719270                                                           15    9605
XP_005268656                                                           15    9605
XP_005268654                                                           15    9605
XP_005268653                                                           15    9605
XP_005251942                                                           15    9605
XP_005251941                                                           15    9605
XP_005270795                                                           15    9605
>NP_443179
          Length = 2170

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QAKEEFASD 9
           QA+EEFASD
Sbjct: 474 QAREEFASD 482
>NP_000421
          Length = 410

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  QAKEEFAS 8
          +AKEEF S
Sbjct: 74 EAKEEFTS 81
>NP_001121371
          Length = 816

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   QAKEEFA 7
           +AKEEFA
Sbjct: 641 EAKEEFA 647
>NP_001269092
          Length = 766

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   QAKEEFA 7
           +AKEEFA
Sbjct: 591 EAKEEFA 597
>NP_001121370
          Length = 816

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   QAKEEFA 7
           +AKEEFA
Sbjct: 641 EAKEEFA 647
>NP_000075
          Length = 746

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   QAKEEFA 7
           +AKEEFA
Sbjct: 571 EAKEEFA 577
>NP_005119
          Length = 2298

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QAKEEFASD 9
            QA E F+SD
Sbjct: 1187 QASESFSSD 1195
>XP_006724131
          Length = 2298

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QAKEEFASD 9
            QA E F+SD
Sbjct: 1187 QASESFSSD 1195
>NP_001274523
          Length = 241

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AKEEFAS 8
           A+EEFAS
Sbjct: 215 AREEFAS 221
>NP_001274522
          Length = 241

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AKEEFAS 8
           A+EEFAS
Sbjct: 215 AREEFAS 221
>NP_599030
          Length = 190

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EEFASD 9
           EEFASD
Sbjct: 137 EEFASD 142
>NP_001279
          Length = 241

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AKEEFAS 8
           A+EEFAS
Sbjct: 215 AREEFAS 221
>NP_001273409
          Length = 834

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AKEEFASD 9
           +K+EF+SD
Sbjct: 524 SKQEFSSD 531
>NP_055598
          Length = 1023

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AKEEFASD 9
           +K+EF+SD
Sbjct: 713 SKQEFSSD 720
>NP_001273408
          Length = 834

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AKEEFASD 9
           +K+EF+SD
Sbjct: 524 SKQEFSSD 531
>XP_006725628
          Length = 994

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AKEEFASD 9
           +K+EF+SD
Sbjct: 684 SKQEFSSD 691
>XP_006725627
          Length = 1023

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AKEEFASD 9
           +K+EF+SD
Sbjct: 713 SKQEFSSD 720
>XP_005257062
          Length = 202

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EEFASD 9
           EEFASD
Sbjct: 137 EEFASD 142
>XP_006720851
          Length = 834

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AKEEFASD 9
           +K+EF+SD
Sbjct: 524 SKQEFSSD 531
>XP_005254865
          Length = 994

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AKEEFASD 9
           +K+EF+SD
Sbjct: 684 SKQEFSSD 691
>NP_001073936
          Length = 1848

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    QAKEEFASD 9
            Q+K+EFA +
Sbjct: 1041 QSKDEFAQN 1049
>NP_057195
          Length = 837

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  KEEFAS 8
          KEEFAS
Sbjct: 51 KEEFAS 56
>XP_006726617
          Length = 1688

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    QAKEEFAS 8
            +AKE+FA+
Sbjct: 1602 EAKEDFAN 1609
>XP_006721056
          Length = 718

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  KEEFAS 8
          KEEFAS
Sbjct: 51 KEEFAS 56
>XP_006720409
          Length = 1688

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    QAKEEFAS 8
            +AKE+FA+
Sbjct: 1602 EAKEDFAN 1609
>XP_006710039
          Length = 1584

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    QAKEEFAS 8
            +AKE+FA+
Sbjct: 1498 EAKEDFAN 1505
>NP_001180360
          Length = 779

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  KEEFASD 9
          KEEFA++
Sbjct: 21 KEEFANE 27
>NP_057051
          Length = 807

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  KEEFASD 9
          KEEFA++
Sbjct: 21 KEEFANE 27
>NP_036543
          Length = 752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  KEEFASD 9
          KEEFA++
Sbjct: 21 KEEFANE 27
>NP_001193727
          Length = 516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  AKEEFASD 9
          A E FASD
Sbjct: 79 ATESFASD 86
>NP_001193725
          Length = 605

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AKEEFASD 9
           A E FASD
Sbjct: 168 ATESFASD 175
>NP_001193728
          Length = 536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AKEEFASD 9
           A E FASD
Sbjct: 99  ATESFASD 106
>NP_872271
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AKEEFASD 9
           A E FASD
Sbjct: 94  ATESFASD 101
>NP_872270
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AKEEFASD 9
           A E FASD
Sbjct: 94  ATESFASD 101
>NP_002645
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AKEEFASD 9
           A E FASD
Sbjct: 94  ATESFASD 101
>NP_001265558
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QAKEEFASD 9
           Q  E+F+SD
Sbjct: 193 QLSEQFSSD 201
>NP_689999
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QAKEEFASD 9
           Q  E+F+SD
Sbjct: 193 QLSEQFSSD 201
>NP_001265559
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QAKEEFASD 9
           Q  E+F+SD
Sbjct: 211 QLSEQFSSD 219
>NP_001248757
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEEFASD 9
           +EEF+SD
Sbjct: 218 QEEFSSD 224
>NP_001248756
          Length = 695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEEFASD 9
           +EEF+SD
Sbjct: 201 QEEFSSD 207
>NP_001073160
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEEFASD 9
           +EEF+SD
Sbjct: 168 QEEFSSD 174
>NP_115986
          Length = 679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEEFASD 9
           +EEF+SD
Sbjct: 185 QEEFSSD 191
>NP_001129465
          Length = 583

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QAKEEFA 7
           QAKE+F+
Sbjct: 552 QAKEDFS 558
>NP_694943
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QAKEEFA 7
           QAKE+F+
Sbjct: 491 QAKEDFS 497
>NP_038477
          Length = 1905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    AKEEFASD 9
            + EEFA+D
Sbjct: 1854 SSEEFAAD 1861
>NP_116242
          Length = 711

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QAKEEFASD 9
           QA+E+F  D
Sbjct: 477 QAREDFNQD 485
>XP_005258255
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QAKEEFA 7
           QAKE+F+
Sbjct: 491 QAKEDFS 497
>XP_005254502
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AKEEFASD 9
           A E FASD
Sbjct: 94  ATESFASD 101
>XP_005254500
          Length = 591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AKEEFASD 9
           A E FASD
Sbjct: 154 ATESFASD 161
>XP_006720633
          Length = 605

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AKEEFASD 9
           A E FASD
Sbjct: 168 ATESFASD 175
>XP_005268661
          Length = 1657

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    AKEEFASD 9
            + EEFA+D
Sbjct: 1606 SSEEFAAD 1613
>XP_005268660
          Length = 1875

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    AKEEFASD 9
            + EEFA+D
Sbjct: 1824 SSEEFAAD 1831
>XP_005268659
          Length = 1894

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    AKEEFASD 9
            + EEFA+D
Sbjct: 1843 SSEEFAAD 1850
>XP_005268657
          Length = 1903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    AKEEFASD 9
            + EEFA+D
Sbjct: 1852 SSEEFAAD 1859
>XP_006719270
          Length = 1903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    AKEEFASD 9
            + EEFA+D
Sbjct: 1852 SSEEFAAD 1859
>XP_005268656
          Length = 1903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    AKEEFASD 9
            + EEFA+D
Sbjct: 1852 SSEEFAAD 1859
>XP_005268654
          Length = 1923

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    AKEEFASD 9
            + EEFA+D
Sbjct: 1872 SSEEFAAD 1879
>XP_005268653
          Length = 1924

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    AKEEFASD 9
            + EEFA+D
Sbjct: 1873 SSEEFAAD 1880
>XP_005251942
          Length = 327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  QAKEEFASD 9
          Q  E+F+SD
Sbjct: 59 QLSEQFSSD 67
>XP_005251941
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QAKEEFASD 9
           Q  E+F+SD
Sbjct: 193 QLSEQFSSD 201
>XP_005270795
          Length = 783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  KEEFASD 9
          KEEFA++
Sbjct: 21 KEEFANE 27
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.295    0.110    0.263 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 555,222
Number of extensions: 624
Number of successful extensions: 61
Number of sequences better than 10000.0: 61
Number of HSP's gapped: 61
Number of HSP's successfully gapped: 61
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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