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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006197                                                              18    1482
XP_006714104                                                           18    1482
XP_005265800                                                           18    1482
XP_006714103                                                           18    1482
XP_006714102                                                           18    1482
NP_060485                                                              18    1935
NP_001171711                                                           18    1935
NP_000531                                                              17    2528
NP_001036188                                                           17    2528
XP_006723382                                                           17    2528
XP_006723381                                                           17    2528
XP_006723380                                                           17    2528
NP_722576                                                              17    4312
XP_005262424                                                           17    4312
NP_071414                                                              17    4312
NP_001177344                                                           16    5631
NP_647476                                                              16    5631
NP_005632                                                              16    5631
XP_006716165                                                           16    5631
XP_006716164                                                           16    5631
XP_006716163                                                           16    5631
NP_000248                                                              16    7355
NP_849157                                                              16    7355
XP_005267754                                                           16    7355
XP_005267753                                                           16    7355
XP_006714159                                                           16    7355
XP_006714158                                                           16    7355
XP_006714157                                                           16    7355
NP_006423                                                              15    9605
NP_112153                                                              15    9605
XP_006720067                                                           15    9605

>NP_006197
          Length = 1089

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PSIKLVSTL 9
           PSIKLV TL
Sbjct: 267 PSIKLVYTL 275



>XP_006714104
          Length = 832

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PSIKLVSTL 9
           PSIKLV TL
Sbjct: 292 PSIKLVYTL 300



>XP_005265800
          Length = 1089

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PSIKLVSTL 9
           PSIKLV TL
Sbjct: 267 PSIKLVYTL 275



>XP_006714103
          Length = 1111

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PSIKLVSTL 9
           PSIKLV TL
Sbjct: 289 PSIKLVYTL 297



>XP_006714102
          Length = 1114

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PSIKLVSTL 9
           PSIKLV TL
Sbjct: 292 PSIKLVYTL 300



>NP_060485
          Length = 855

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   PSIKLVSTL 9
           P IKL+ST+
Sbjct: 318 PQIKLISTM 326



>NP_001171711
          Length = 758

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   PSIKLVSTL 9
           P IKL+ST+
Sbjct: 318 PQIKLISTM 326



>NP_000531
          Length = 5038

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    PSIKLVSTL 9
            P +KLVSTL
Sbjct: 1840 PVLKLVSTL 1848



>NP_001036188
          Length = 5033

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    PSIKLVSTL 9
            P +KLVSTL
Sbjct: 1840 PVLKLVSTL 1848



>XP_006723382
          Length = 5027

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    PSIKLVSTL 9
            P +KLVSTL
Sbjct: 1840 PVLKLVSTL 1848



>XP_006723381
          Length = 5031

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    PSIKLVSTL 9
            P +KLVSTL
Sbjct: 1839 PVLKLVSTL 1847



>XP_006723380
          Length = 5032

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    PSIKLVSTL 9
            P +KLVSTL
Sbjct: 1840 PVLKLVSTL 1848



>NP_722576
          Length = 3080

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PSIKLVSTL 9
           PS+ ++STL
Sbjct: 784 PSVDIISTL 792



>XP_005262424
          Length = 2875

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PSIKLVSTL 9
           PS+ ++STL
Sbjct: 579 PSVDIISTL 587



>NP_071414
          Length = 955

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   PSIKLVST 8
           P IK+VST
Sbjct: 660 PDIKIVST 667



>NP_001177344
          Length = 714

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   PSIKLVST 8
           P +KLVST
Sbjct: 617 PIVKLVST 624



>NP_647476
          Length = 677

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   PSIKLVST 8
           P +KLVST
Sbjct: 580 PIVKLVST 587



>NP_005632
          Length = 677

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   PSIKLVST 8
           P +KLVST
Sbjct: 580 PIVKLVST 587



>XP_006716165
          Length = 667

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   PSIKLVST 8
           P +KLVST
Sbjct: 570 PIVKLVST 577



>XP_006716164
          Length = 667

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   PSIKLVST 8
           P +KLVST
Sbjct: 570 PIVKLVST 577



>XP_006716163
          Length = 677

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   PSIKLVST 8
           P +KLVST
Sbjct: 580 PIVKLVST 587



>NP_000248
          Length = 1935

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SIKLVSTL 9
           S+KL+STL
Sbjct: 613 SLKLLSTL 620



>NP_849157
          Length = 1077

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SIKLVST 8
           S+KLVST
Sbjct: 868 SVKLVST 874



>XP_005267754
          Length = 1087

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SIKLVSTL 9
           S+KL+STL
Sbjct: 613 SLKLLSTL 620



>XP_005267753
          Length = 1935

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SIKLVSTL 9
           S+KL+STL
Sbjct: 613 SLKLLSTL 620



>XP_006714159
          Length = 1077

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SIKLVST 8
           S+KLVST
Sbjct: 868 SVKLVST 874



>XP_006714158
          Length = 1077

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SIKLVST 8
           S+KLVST
Sbjct: 868 SVKLVST 874



>XP_006714157
          Length = 1077

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SIKLVST 8
           S+KLVST
Sbjct: 868 SVKLVST 874



>NP_006423
          Length = 151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   PSIKLV 6
           PSIKLV
Sbjct: 120 PSIKLV 125



>NP_112153
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  PSIKLVST 8
          PS+ LVST
Sbjct: 9  PSLLLVST 16



>XP_006720067
          Length = 174

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   PSIKLV 6
           PSIKLV
Sbjct: 120 PSIKLV 125



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.129    0.324 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 346,711
Number of extensions: 462
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)