Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005179                                                              20     299
NP_733762                                                              18    1935
XP_006713860                                                           18    1935
XP_006713859                                                           18    1935
XP_005247910                                                           18    1935
XP_005247909                                                           18    1935
NP_001273560                                                           16    5631
NP_004450                                                              16    5631
NP_872579                                                              16    5631
XP_005257218                                                           16    5631
XP_005257217                                                           16    5631
XP_005257216                                                           16    5631
XP_005257215                                                           16    5631
XP_005257214                                                           16    5631
XP_005257213                                                           16    5631
XP_005257212                                                           16    5631
XP_006721834                                                           16    5631
XP_005257211                                                           16    5631
XP_005257210                                                           16    5631
XP_005257209                                                           16    5631
XP_005257208                                                           16    5631
XP_006721833                                                           16    5631
XP_005257207                                                           16    5631
XP_006719923                                                           16    5631
XP_006719922                                                           16    5631
XP_006719920                                                           16    5631
XP_005266554                                                           16    5631
XP_005266553                                                           16    5631
XP_006719919                                                           16    5631
XP_005266551                                                           16    5631
XP_005266550                                                           16    5631
XP_005266549                                                           16    5631
XP_006719918                                                           16    5631
XP_005266548                                                           16    5631
XP_005270828                                                           16    5631
NP_000294                                                              16    7355
XP_005255429                                                           16    7355
XP_005267366                                                           16    7355
XP_006720083                                                           16    7355
XP_006720082                                                           16    7355
XP_006712555                                                           16    7355
XP_006712554                                                           16    7355
XP_005263717                                                           16    7355
XP_005263716                                                           16    7355
XP_006712553                                                           16    7355
NP_694591                                                              16    7355
NP_997246                                                              16    7355
NP_619542                                                              15    9605
NP_001273484                                                           15    9605
NP_001273759                                                           15    9605
NP_079040                                                              15    9605
NP_064570                                                              15    9605
XP_005274600                                                           15    9605
>NP_005179
          Length = 906

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PRQNLTKLS 9
           PR+NLTKLS
Sbjct: 147 PRRNLTKLS 155
>NP_733762
          Length = 982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   RQNLTKLS 9
           R+NLTKLS
Sbjct: 140 RRNLTKLS 147
>XP_006713860
          Length = 914

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   RQNLTKLS 9
           R+NLTKLS
Sbjct: 168 RRNLTKLS 175
>XP_006713859
          Length = 966

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   RQNLTKLS 9
           R+NLTKLS
Sbjct: 168 RRNLTKLS 175
>XP_005247910
          Length = 982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   RQNLTKLS 9
           R+NLTKLS
Sbjct: 140 RRNLTKLS 147
>XP_005247909
          Length = 1010

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   RQNLTKLS 9
           R+NLTKLS
Sbjct: 168 RRNLTKLS 175
>NP_001273560
          Length = 1009

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QNLTKLS 9
           QN+TKLS
Sbjct: 664 QNVTKLS 670
>NP_004450
          Length = 2903

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2588 KQNATKLS 2595
>NP_872579
          Length = 2920

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2605 KQNATKLS 2612
>XP_005257218
          Length = 2957

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2642 KQNATKLS 2649
>XP_005257217
          Length = 2961

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2588 KQNATKLS 2595
>XP_005257216
          Length = 3015

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2642 KQNATKLS 2649
>XP_005257215
          Length = 3032

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2659 KQNATKLS 2666
>XP_005257214
          Length = 3094

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2721 KQNATKLS 2728
>XP_005257213
          Length = 3095

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2722 KQNATKLS 2729
>XP_005257212
          Length = 3095

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2722 KQNATKLS 2729
>XP_006721834
          Length = 3100

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2785 KQNATKLS 2792
>XP_005257211
          Length = 3100

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2785 KQNATKLS 2792
>XP_005257210
          Length = 3104

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2731 KQNATKLS 2738
>XP_005257209
          Length = 3148

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2785 KQNATKLS 2792
>XP_005257208
          Length = 3157

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2784 KQNATKLS 2791
>XP_006721833
          Length = 3158

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2785 KQNATKLS 2792
>XP_005257207
          Length = 3158

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RQNLTKLS 9
            +QN TKLS
Sbjct: 2785 KQNATKLS 2792
>XP_006719923
          Length = 734

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QNLTKLS 9
           QN+TKLS
Sbjct: 389 QNVTKLS 395
>XP_006719922
          Length = 942

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QNLTKLS 9
           QN+TKLS
Sbjct: 677 QNVTKLS 683
>XP_006719920
          Length = 999

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QNLTKLS 9
           QN+TKLS
Sbjct: 677 QNVTKLS 683
>XP_005266554
          Length = 1000

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QNLTKLS 9
           QN+TKLS
Sbjct: 677 QNVTKLS 683
>XP_005266553
          Length = 1007

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QNLTKLS 9
           QN+TKLS
Sbjct: 662 QNVTKLS 668
>XP_006719919
          Length = 1009

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QNLTKLS 9
           QN+TKLS
Sbjct: 664 QNVTKLS 670
>XP_005266551
          Length = 1012

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QNLTKLS 9
           QN+TKLS
Sbjct: 667 QNVTKLS 673
>XP_005266550
          Length = 1017

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QNLTKLS 9
           QN+TKLS
Sbjct: 672 QNVTKLS 678
>XP_005266549
          Length = 1019

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QNLTKLS 9
           QN+TKLS
Sbjct: 674 QNVTKLS 680
>XP_006719918
          Length = 1021

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QNLTKLS 9
           QN+TKLS
Sbjct: 676 QNVTKLS 682
>XP_005266548
          Length = 1022

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QNLTKLS 9
           QN+TKLS
Sbjct: 677 QNVTKLS 683
>XP_005270828
          Length = 444

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PRQNLTKLS 9
           PR  LT+LS
Sbjct: 125 PRPRLTRLS 133
>NP_000294
          Length = 246

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  PRQNLTK 7
          PRQ +TK
Sbjct: 20 PRQKITK 26
>XP_005255429
          Length = 270

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  PRQNLTK 7
          PRQ +TK
Sbjct: 20 PRQKITK 26
>XP_005267366
          Length = 1330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PRQNLTKLS 9
           PR +L KLS
Sbjct: 109 PRPSLAKLS 117
>XP_006720083
          Length = 1355

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PRQNLTKLS 9
           PR +L KLS
Sbjct: 109 PRPSLAKLS 117
>XP_006720082
          Length = 1363

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PRQNLTKLS 9
           PR +L KLS
Sbjct: 109 PRPSLAKLS 117
>XP_006712555
          Length = 1786

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RQNLTKL 8
           +QNLTK+
Sbjct: 630 KQNLTKI 636
>XP_006712554
          Length = 1885

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RQNLTKL 8
           +QNLTK+
Sbjct: 729 KQNLTKI 735
>XP_005263717
          Length = 1953

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RQNLTKL 8
           +QNLTK+
Sbjct: 797 KQNLTKI 803
>XP_005263716
          Length = 1953

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RQNLTKL 8
           +QNLTK+
Sbjct: 797 KQNLTKI 803
>XP_006712553
          Length = 1953

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RQNLTKL 8
           +QNLTK+
Sbjct: 797 KQNLTKI 803
>NP_694591
          Length = 1382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PRQNLTKLS 9
           PR +L KLS
Sbjct: 109 PRPSLAKLS 117
>NP_997246
          Length = 1909

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RQNLTKL 8
           +QNLTK+
Sbjct: 797 KQNLTKI 803
>NP_619542
          Length = 1041

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QNLTKLS 9
          QNLTK++
Sbjct: 87 QNLTKIN 93
>NP_001273484
          Length = 205

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  PRQNLTK 7
          P QNLT+
Sbjct: 59 PSQNLTR 65
>NP_001273759
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  PRQNLTK 7
          PR+ LTK
Sbjct: 13 PREKLTK 19
>NP_079040
          Length = 1116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   PRQNLTK 7
           PR+N+T+
Sbjct: 115 PRENITQ 121
>NP_064570
          Length = 184

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  PRQNLTK 7
          P QNLT+
Sbjct: 59 PSQNLTR 65
>XP_005274600
          Length = 1059

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QNLTKLS 9
           QNLTK++
Sbjct: 105 QNLTKIN 111
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.123    0.330 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 476,692
Number of extensions: 961
Number of successful extensions: 53
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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