Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002871                                                              19     665
XP_005265416                                                           19     665
XP_005265415                                                           19     665
XP_005265414                                                           19     665
XP_005265412                                                           19     665
NP_004324                                                              19     869
XP_005250102                                                           19     869
NP_001243125                                                           18    1935
NP_001645                                                              18    1935
XP_006724592                                                           18    1935
XP_006722488                                                           17    2528
XP_006722487                                                           17    2528
XP_006722486                                                           17    2528
XP_006722485                                                           17    2528
XP_005266720                                                           17    2528
XP_006722484                                                           17    2528
XP_005266717                                                           17    2528
XP_005266715                                                           17    2528
NP_116098                                                              17    4312
XP_006718108                                                           17    4312
NP_291025                                                              16    5631
NP_620162                                                              16    7355
NP_001271330                                                           16    7355
NP_653267                                                              16    7355
XP_005250103                                                           16    7355
XP_005264932                                                           16    7355
XP_006713036                                                           16    7355
XP_005264930                                                           16    7355
NP_001185617                                                           15    9605
NP_001003680                                                           15    9605
NP_001185616                                                           15    9605
NP_002294                                                              15    9605
NP_001003679                                                           15    9605
NP_001185618                                                           15    9605
NP_113678                                                              15    9605
>NP_002871
          Length = 648

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PQILTSIEL 9
           PQIL+SIEL
Sbjct: 600 PQILSSIEL 608
>XP_005265416
          Length = 534

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PQILTSIEL 9
           PQIL+SIEL
Sbjct: 486 PQILSSIEL 494
>XP_005265415
          Length = 567

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PQILTSIEL 9
           PQIL+SIEL
Sbjct: 519 PQILSSIEL 527
>XP_005265414
          Length = 615

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PQILTSIEL 9
           PQIL+SIEL
Sbjct: 567 PQILSSIEL 575
>XP_005265412
          Length = 648

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PQILTSIEL 9
           PQIL+SIEL
Sbjct: 600 PQILSSIEL 608
>NP_004324
          Length = 766

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PQILTSIEL 9
           PQIL SIEL
Sbjct: 708 PQILASIEL 716

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   PQILTS 6
           PQILTS
Sbjct: 328 PQILTS 333
>XP_005250102
          Length = 767

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PQILTSIEL 9
           PQIL SIEL
Sbjct: 708 PQILASIEL 716

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   PQILTS 6
           PQILTS
Sbjct: 328 PQILTS 333
>NP_001243125
          Length = 609

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   PQILTSIEL 9
           PQIL +IEL
Sbjct: 564 PQILATIEL 572
>NP_001645
          Length = 606

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   PQILTSIEL 9
           PQIL +IEL
Sbjct: 561 PQILATIEL 569
>XP_006724592
          Length = 611

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   PQILTSIEL 9
           PQIL +IEL
Sbjct: 566 PQILATIEL 574
>XP_006722488
          Length = 1084

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  PQILTSIE 8
          PQ+LTSI+
Sbjct: 75 PQLLTSID 82
>XP_006722487
          Length = 1102

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  PQILTSIE 8
          PQ+LTSI+
Sbjct: 75 PQLLTSID 82
>XP_006722486
          Length = 1150

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  PQILTSIE 8
          PQ+LTSI+
Sbjct: 75 PQLLTSID 82
>XP_006722485
          Length = 1168

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  PQILTSIE 8
          PQ+LTSI+
Sbjct: 75 PQLLTSID 82
>XP_005266720
          Length = 1234

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  PQILTSIE 8
          PQ+LTSI+
Sbjct: 75 PQLLTSID 82
>XP_006722484
          Length = 1252

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  PQILTSIE 8
          PQ+LTSI+
Sbjct: 75 PQLLTSID 82
>XP_005266717
          Length = 1254

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  PQILTSIE 8
          PQ+LTSI+
Sbjct: 75 PQLLTSID 82
>XP_005266715
          Length = 1272

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  PQILTSIE 8
          PQ+LTSI+
Sbjct: 75 PQLLTSID 82
>NP_116098
          Length = 581

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PQILTSIEL 9
           PQI T +EL
Sbjct: 96  PQIYTDLEL 104
>XP_006718108
          Length = 560

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PQILTSIEL 9
           PQI T +EL
Sbjct: 96  PQIYTDLEL 104
>NP_291025
          Length = 963

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   PQILTSIE 8
           PQ+ TSIE
Sbjct: 936 PQVETSIE 943
>NP_620162
          Length = 316

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  PQILTSIE 8
          P+I+TSI+
Sbjct: 81 PKIITSIQ 88
>NP_001271330
          Length = 337

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  PQILTSIE 8
          P+I+TSI+
Sbjct: 81 PKIITSIQ 88
>NP_653267
          Length = 4753

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    PQILTSIEL 9
            P +LT++EL
Sbjct: 3687 PVLLTNVEL 3695
>XP_005250103
          Length = 758

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   PQILTS 6
           PQILTS
Sbjct: 328 PQILTS 333
>XP_005264932
          Length = 189

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  PQILTSIE 8
          P+I+TSI+
Sbjct: 81 PKIITSIQ 88
>XP_006713036
          Length = 295

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  PQILTSIE 8
          P+I+TSI+
Sbjct: 81 PKIITSIQ 88
>XP_005264930
          Length = 452

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  PQILTSIE 8
          P+I+TSI+
Sbjct: 81 PKIITSIQ 88
>NP_001185617
          Length = 906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PQILTSI 7
           P+ILTS+
Sbjct: 338 PKILTSV 344
>NP_001003680
          Length = 958

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PQILTSI 7
           P+ILTS+
Sbjct: 338 PKILTSV 344
>NP_001185616
          Length = 958

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PQILTSI 7
           P+ILTS+
Sbjct: 338 PKILTSV 344
>NP_002294
          Length = 1165

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PQILTSI 7
           P+ILTS+
Sbjct: 338 PKILTSV 344
>NP_001003679
          Length = 896

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PQILTSI 7
           P+ILTS+
Sbjct: 338 PKILTSV 344
>NP_001185618
          Length = 896

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PQILTSI 7
           P+ILTS+
Sbjct: 338 PKILTSV 344
>NP_113678
          Length = 852

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   PQILTSI 7
           P ILTSI
Sbjct: 184 PDILTSI 190
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.137    0.350 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 449,509
Number of extensions: 815
Number of successful extensions: 37
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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