Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              18    1135
NP_001153059                                                           18    1482
NP_001153060                                                           18    1482
NP_598000                                                              18    1482
XP_005248716                                                           18    1482
NP_057440                                                              17    3301
NP_001182539                                                           17    3301
NP_064581                                                              17    3301
NP_036587                                                              17    4312
NP_919267                                                              17    4312
XP_006712457                                                           17    4312
XP_006712456                                                           17    4312
XP_006712455                                                           17    4312
XP_005263691                                                           17    4312
NP_065970                                                              16    5631
NP_036560                                                              16    5631
NP_001154836                                                           16    5631
NP_001154835                                                           16    5631
NP_059429                                                              16    5631
XP_005254361                                                           16    5631
XP_006712718                                                           16    5631
XP_005246767                                                           16    5631
XP_005246766                                                           16    5631
XP_006712717                                                           16    5631
NP_001239155                                                           16    7355
NP_006613                                                              16    7355
NP_997320                                                              16    7355
NP_062826                                                              16    7355
NP_996801                                                              16    7355
XP_006720106                                                           16    7355
XP_005253612                                                           16    7355
NP_001074299                                                           15    9605
NP_001013764                                                           15    9605
NP_001265667                                                           15    9605
NP_057677                                                              15    9605
NP_001265668                                                           15    9605
NP_001094385                                                           15    9605
XP_005255865                                                           15    9605
XP_006721206                                                           15    9605
XP_005249228                                                           15    9605
>NP_004351
          Length = 882

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PMEILNTVT 9
           PMEIL TVT
Sbjct: 245 PMEILITVT 253
>NP_001153059
          Length = 1428

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   PMEILNTVT 9
           P+EIL TVT
Sbjct: 320 PVEILTTVT 328
>NP_001153060
          Length = 1368

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   PMEILNTVT 9
           P+EIL TVT
Sbjct: 243 PVEILTTVT 251
>NP_598000
          Length = 1445

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   PMEILNTVT 9
           P+EIL TVT
Sbjct: 320 PVEILTTVT 328
>XP_005248716
          Length = 1445

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   PMEILNTVT 9
           P+EIL TVT
Sbjct: 320 PVEILTTVT 328
>NP_057440
          Length = 476

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PMEILNT 7
           PME++NT
Sbjct: 109 PMEVVNT 115
>NP_001182539
          Length = 476

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PMEILNT 7
           PME++NT
Sbjct: 109 PMEVVNT 115
>NP_064581
          Length = 855

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PMEILNTVT 9
           P EI+NT T
Sbjct: 371 PREIINTYT 379
>NP_036587
          Length = 1056

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  PMEILN 6
          PMEILN
Sbjct: 13 PMEILN 18
>NP_919267
          Length = 240

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  PMEILNT 7
          PME LNT
Sbjct: 62 PMEALNT 68
>XP_006712457
          Length = 964

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  PMEILN 6
          PMEILN
Sbjct: 13 PMEILN 18
>XP_006712456
          Length = 965

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  PMEILN 6
          PMEILN
Sbjct: 13 PMEILN 18
>XP_006712455
          Length = 1055

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  PMEILN 6
          PMEILN
Sbjct: 13 PMEILN 18
>XP_005263691
          Length = 1056

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  PMEILN 6
          PMEILN
Sbjct: 13 PMEILN 18
>NP_065970
          Length = 1657

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MEILNTV 8
           MEILNT+
Sbjct: 830 MEILNTL 836
>NP_036560
          Length = 398

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PMEILNT 7
           PM+I+NT
Sbjct: 279 PMDIVNT 285
>NP_001154836
          Length = 278

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PMEILNT 7
           PM+I+NT
Sbjct: 163 PMDIVNT 169
>NP_001154835
          Length = 394

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PMEILNT 7
           PM+I+NT
Sbjct: 279 PMDIVNT 285
>NP_059429
          Length = 282

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PMEILNT 7
           PM+I+NT
Sbjct: 163 PMDIVNT 169
>XP_005254361
          Length = 376

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PMEILNT 7
           PM+I+NT
Sbjct: 279 PMDIVNT 285
>XP_006712718
          Length = 969

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MEILNTV 8
           MEILNT+
Sbjct: 142 MEILNTL 148
>XP_005246767
          Length = 1356

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MEILNTV 8
           MEILNT+
Sbjct: 830 MEILNTL 836
>XP_005246766
          Length = 1656

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MEILNTV 8
           MEILNT+
Sbjct: 830 MEILNTL 836
>XP_006712717
          Length = 1657

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MEILNTV 8
           MEILNT+
Sbjct: 830 MEILNTL 836
>NP_001239155
          Length = 671

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2 MEILNTVT 9
         ME+L T+T
Sbjct: 1 MELLRTIT 8
>NP_006613
          Length = 685

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2 MEILNTVT 9
         ME+L T+T
Sbjct: 1 MELLRTIT 8
>NP_997320
          Length = 4471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    MEILNT 7
            MEILNT
Sbjct: 4057 MEILNT 4062
>NP_062826
          Length = 580

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MEILNTVT 9
           +E+LNT T
Sbjct: 247 LELLNTTT 254
>NP_996801
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MEILNTV 8
           ME+LNT+
Sbjct: 185 MEVLNTL 191
>XP_006720106
          Length = 287

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MEILNTV 8
           ME+LNT+
Sbjct: 149 MEVLNTL 155
>XP_005253612
          Length = 4589

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    MEILNT 7
            MEILNT
Sbjct: 4175 MEILNT 4180
>NP_001074299
          Length = 483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MEILNTV 8
           +E+LNTV
Sbjct: 194 IEVLNTV 200
>NP_001013764
          Length = 1271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PMEILNTV 8
           P++ILN V
Sbjct: 598 PLDILNRV 605
>NP_001265667
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PMEILNTV 8
           P+EILN +
Sbjct: 331 PVEILNYI 338
>NP_057677
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PMEILNTV 8
           P+EILN +
Sbjct: 351 PVEILNYI 358
>NP_001265668
          Length = 297

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PMEILNTV 8
           P+EILN +
Sbjct: 179 PVEILNYI 186
>NP_001094385
          Length = 926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PMEILNTVT 9
           PM++LN  T
Sbjct: 378 PMDVLNEWT 386
>XP_005255865
          Length = 917

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PMEILNTVT 9
           PM++LN  T
Sbjct: 378 PMDVLNEWT 386
>XP_006721206
          Length = 921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PMEILNTVT 9
           PM++LN  T
Sbjct: 373 PMDVLNEWT 381
>XP_005249228
          Length = 420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PMEILNTV 8
           P+EILN +
Sbjct: 351 PVEILNYI 358
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.130    0.351 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 468,008
Number of extensions: 649
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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