Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              20     509
NP_001119584                                                           20     509
NP_001119586                                                           20     509
NP_001119585                                                           20     509
NP_002244                                                              17    4312
NP_775100                                                              16    5631
NP_060890                                                              16    5631
NP_937793                                                              16    5631
XP_005271961                                                           16    5631
XP_006710817                                                           16    5631
XP_005271062                                                           16    5631
XP_006710816                                                           16    5631
NP_997010                                                              16    5631
NP_055178                                                              16    7355
NP_001264984                                                           16    7355
NP_115871                                                              16    7355
XP_006722986                                                           16    7355
XP_005259717                                                           16    7355
XP_005259716                                                           16    7355
XP_005266395                                                           16    7355
NP_612459                                                              15    9605
NP_001264993                                                           15    9605
NP_056483                                                              15    9605
NP_055832                                                              15    9605
NP_439896                                                              15    9605
NP_203133                                                              15    9605
NP_963920                                                              15    9605
NP_963918                                                              15    9605
NP_001273621                                                           15    9605
XP_006723827                                                           15    9605
XP_005260415                                                           15    9605
XP_005266310                                                           15    9605
XP_005250917                                                           15    9605
XP_006716604                                                           15    9605
XP_005250916                                                           15    9605
XP_005250915                                                           15    9605
XP_005250914                                                           15    9605
XP_005250913                                                           15    9605
XP_006715391                                                           15    9605
XP_005270706                                                           15    9605
XP_005270705                                                           15    9605
NP_001001711                                                           15    9605
>NP_000537
          Length = 393

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  PLSLETFSD 9
          PLS ETFSD
Sbjct: 13 PLSQETFSD 21
>NP_001119584
          Length = 393

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  PLSLETFSD 9
          PLS ETFSD
Sbjct: 13 PLSQETFSD 21
>NP_001119586
          Length = 341

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  PLSLETFSD 9
          PLS ETFSD
Sbjct: 13 PLSQETFSD 21
>NP_001119585
          Length = 346

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  PLSLETFSD 9
          PLS ETFSD
Sbjct: 13 PLSQETFSD 21
>NP_002244
          Length = 1356

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    PLSLETFSD 9
            P+S++TF D
Sbjct: 1233 PVSVKTFED 1241
>NP_775100
          Length = 374

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  PLSLETFS 8
          P SLET+S
Sbjct: 39 PWSLETYS 46
>NP_060890
          Length = 547

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LETFSD 9
           LETFSD
Sbjct: 490 LETFSD 495
>NP_937793
          Length = 536

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PLSLETFSD 9
           PLS ETF +
Sbjct: 383 PLSSETFKN 391
>XP_005271961
          Length = 377

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PLSLETFSD 9
           PLS ETF +
Sbjct: 224 PLSSETFKN 232
>XP_006710817
          Length = 315

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LETFSD 9
           LETFSD
Sbjct: 258 LETFSD 263
>XP_005271062
          Length = 316

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LETFSD 9
           LETFSD
Sbjct: 259 LETFSD 264
>XP_006710816
          Length = 476

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LETFSD 9
           LETFSD
Sbjct: 419 LETFSD 424
>NP_997010
          Length = 325

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   PLSLETFS 8
           PL L TFS
Sbjct: 288 PLGLSTFS 295
>NP_055178
          Length = 4579

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    PLSLET 6
            PLSLET
Sbjct: 2872 PLSLET 2877
>NP_001264984
          Length = 4432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    PLSLET 6
            PLSLET
Sbjct: 2725 PLSLET 2730
>NP_115871
          Length = 1537

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSLETFSD 9
            LSL +FSD
Sbjct: 1326 LSLHSFSD 1333
>XP_006722986
          Length = 1519

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSLETFSD 9
            LSL +FSD
Sbjct: 1107 LSLHSFSD 1114
>XP_005259717
          Length = 1738

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSLETFSD 9
            LSL +FSD
Sbjct: 1326 LSLHSFSD 1333
>XP_005259716
          Length = 1738

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSLETFSD 9
            LSL +FSD
Sbjct: 1326 LSLHSFSD 1333
>XP_005266395
          Length = 4588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    PLSLET 6
            PLSLET
Sbjct: 2881 PLSLET 2886
>NP_612459
          Length = 196

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LSLETFSD 9
           LSLE F D
Sbjct: 139 LSLERFQD 146
>NP_001264993
          Length = 1194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    PLSLETFSD 9
            PL L TF +
Sbjct: 1079 PLQLSTFGE 1087
>NP_056483
          Length = 1264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSLETFSD 9
          LSL TFS+
Sbjct: 36 LSLPTFSE 43
>NP_055832
          Length = 942

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LSLETFSD 9
           ++LETF D
Sbjct: 497 VTLETFQD 504
>NP_439896
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SLETFS 8
           SLETFS
Sbjct: 169 SLETFS 174
>NP_203133
          Length = 307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LSLETFSD 9
           L++ETFS+
Sbjct: 251 LAVETFSE 258
>NP_963920
          Length = 828

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LSLETFSD 9
           ++LETF D
Sbjct: 497 VTLETFQD 504
>NP_963918
          Length = 911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LSLETFSD 9
           ++LETF D
Sbjct: 466 VTLETFQD 473
>NP_001273621
          Length = 1252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSLETFSD 9
          LSL TFS+
Sbjct: 36 LSLPTFSE 43
>XP_006723827
          Length = 1208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  PLSLETFS 8
          PLS  TFS
Sbjct: 18 PLSSRTFS 25
>XP_005260415
          Length = 1233

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  PLSLETFS 8
          PLS  TFS
Sbjct: 18 PLSSRTFS 25
>XP_005266310
          Length = 196

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LSLETFSD 9
           LSLE F D
Sbjct: 139 LSLERFQD 146
>XP_005250917
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSLETFSD 9
          LSL TFS+
Sbjct: 36 LSLPTFSE 43
>XP_006716604
          Length = 1045

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSLETFSD 9
          LSL TFS+
Sbjct: 36 LSLPTFSE 43
>XP_005250916
          Length = 1099

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSLETFSD 9
          LSL TFS+
Sbjct: 36 LSLPTFSE 43
>XP_005250915
          Length = 1210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSLETFSD 9
          LSL TFS+
Sbjct: 36 LSLPTFSE 43
>XP_005250914
          Length = 1211

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSLETFSD 9
          LSL TFS+
Sbjct: 36 LSLPTFSE 43
>XP_005250913
          Length = 1211

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LSLETFSD 9
          LSL TFS+
Sbjct: 36 LSLPTFSE 43
>XP_006715391
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SLETFS 8
           SLETFS
Sbjct: 169 SLETFS 174
>XP_005270706
          Length = 851

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LSLETFSD 9
           ++LETF D
Sbjct: 497 VTLETFQD 504
>XP_005270705
          Length = 934

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LSLETFSD 9
           ++LETF D
Sbjct: 497 VTLETFQD 504
>NP_001001711
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SLETFS 8
           SLETFS
Sbjct: 175 SLETFS 180
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.130    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 529,176
Number of extensions: 1315
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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