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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001177396                                                           18    1935
NP_060743                                                              17    2528
NP_001264198                                                           16    7355
NP_001900                                                              15    9605
NP_060264                                                              15    9605
NP_001159450                                                           15    9605
NP_653308                                                              15    9605
NP_001159449                                                           15    9605
NP_000115                                                              15    9605
XP_005251551                                                           15    9605
XP_006716857                                                           15    9605
XP_005263936                                                           15    9605

>NP_001177396
          Length = 1346

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PLALSSLAA 9
           PLALSSL A
Sbjct: 605 PLALSSLQA 613



>NP_060743
          Length = 459

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  PLALSSLAA 9
          P ALSSLAA
Sbjct: 41 PRALSSLAA 49



>NP_001264198
          Length = 2925

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    PLALSSLAA 9
            P  LSSLAA
Sbjct: 1254 PYQLSSLAA 1262



>NP_001900
          Length = 412

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  PLALSSLAA 9
          PLAL  LAA
Sbjct: 8  PLALCLLAA 16



>NP_060264
          Length = 1801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   LALSSLA 8
           LALSSLA
Sbjct: 962 LALSSLA 968



>NP_001159450
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  LALSSLAA 9
          LALS LAA
Sbjct: 18 LALSQLAA 25



>NP_653308
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  LALSSLAA 9
          LALS LAA
Sbjct: 18 LALSQLAA 25



>NP_001159449
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  LALSSLAA 9
          LALS LAA
Sbjct: 18 LALSQLAA 25



>NP_000115
          Length = 1493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    PLALSSLAA 9
            PLA SSL A
Sbjct: 1383 PLASSSLLA 1391



>XP_005251551
          Length = 1801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   LALSSLA 8
           LALSSLA
Sbjct: 962 LALSSLA 968



>XP_006716857
          Length = 1834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2    LALSSLA 8
            LALSSLA
Sbjct: 995  LALSSLA 1001



>XP_005263936
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  LALSSLAA 9
          LALS LAA
Sbjct: 18 LALSQLAA 25



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.119    0.291 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 302,113
Number of extensions: 496
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)