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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              18    1135
XP_005262424                                                           18    1135
NP_001161693                                                           18    1935
XP_005246404                                                           18    1935
NP_660155                                                              17    3301
XP_005270495                                                           17    3301
XP_006710403                                                           17    3301
NP_061758                                                              17    4312
NP_115267                                                              17    4312
NP_061746                                                              17    4312
NP_667338                                                              16    5631
NP_001159608                                                           16    5631
NP_079485                                                              16    5631
NP_001258933                                                           16    5631
NP_001269320                                                           16    5631
NP_055061                                                              16    5631
NP_001258948                                                           16    5631
XP_006724446                                                           16    5631
XP_006717573                                                           16    5631
XP_006713923                                                           16    5631
XP_006713922                                                           16    5631
NP_001107563                                                           16    7355
NP_001247                                                              16    7355
XP_006722284                                                           16    7355
XP_006722283                                                           16    7355
XP_006722282                                                           16    7355
XP_005257952                                                           16    7355
XP_006722281                                                           16    7355
XP_006722280                                                           16    7355
XP_005257949                                                           16    7355
NP_003728                                                              15    9605
NP_061916                                                              15    9605
XP_005253264                                                           15    9605
XP_006717514                                                           15    9605

>NP_722576
          Length = 3080

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PKSATTQIL 9
           PKSATTQ L
Sbjct: 827 PKSATTQRL 835



>XP_005262424
          Length = 2875

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PKSATTQIL 9
           PKSATTQ L
Sbjct: 622 PKSATTQRL 630



>NP_001161693
          Length = 1820

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   PKSATTQIL 9
           P+SAT+QI+
Sbjct: 922 PRSATSQII 930



>XP_005246404
          Length = 1821

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   PKSATTQIL 9
           P+SAT+QI+
Sbjct: 923 PRSATSQII 931



>NP_660155
          Length = 891

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   PKSATTQ 7
           PK+ATTQ
Sbjct: 250 PKNATTQ 256



>XP_005270495
          Length = 517

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   PKSATTQ 7
           PK+ATTQ
Sbjct: 250 PKNATTQ 256



>XP_006710403
          Length = 878

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   PKSATTQ 7
           PK+ATTQ
Sbjct: 237 PKNATTQ 243



>NP_061758
          Length = 787

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PKSATTQIL 9
           P+S T QIL
Sbjct: 221 PRSGTVQIL 229



>NP_115267
          Length = 811

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   PKSATTQI 8
           P+S TTQI
Sbjct: 221 PQSGTTQI 228



>NP_061746
          Length = 931

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   PKSATTQI 8
           P+S TTQI
Sbjct: 221 PQSGTTQI 228



>NP_667338
          Length = 1308

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    KSATTQIL 9
            KSA TQIL
Sbjct: 1007 KSACTQIL 1014



>NP_001159608
          Length = 1228

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KSATTQIL 9
           KSA TQIL
Sbjct: 927 KSACTQIL 934



>NP_079485
          Length = 836

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SATTQIL 9
           SATTQ+L
Sbjct: 744 SATTQVL 750



>NP_001258933
          Length = 813

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SATTQIL 9
           SATTQ+L
Sbjct: 721 SATTQVL 727



>NP_001269320
          Length = 763

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SATTQIL 9
           SATTQ+L
Sbjct: 671 SATTQVL 677



>NP_055061
          Length = 3014

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   PKSATTQI 8
           P+SATT I
Sbjct: 332 PRSATTYI 339



>NP_001258948
          Length = 742

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SATTQIL 9
           SATTQ+L
Sbjct: 650 SATTQVL 656



>XP_006724446
          Length = 3019

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   PKSATTQI 8
           P+SATT I
Sbjct: 332 PRSATTYI 339



>XP_006717573
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SATTQIL 9
           SATTQ+L
Sbjct: 694 SATTQVL 700



>XP_006713923
          Length = 1228

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KSATTQIL 9
           KSA TQIL
Sbjct: 927 KSACTQIL 934



>XP_006713922
          Length = 1262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    KSATTQIL 9
            KSA TQIL
Sbjct: 1007 KSACTQIL 1014



>NP_001107563
          Length = 830

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKSATTQI 8
           P S TTQ+
Sbjct: 181 PNSCTTQV 188



>NP_001247
          Length = 824

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKSATTQI 8
           P S TTQ+
Sbjct: 181 PNSCTTQV 188



>XP_006722284
          Length = 689

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  PKSATTQI 8
          P S TTQ+
Sbjct: 46 PNSCTTQV 53



>XP_006722283
          Length = 742

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKSATTQI 8
           P S TTQ+
Sbjct: 99  PNSCTTQV 106



>XP_006722282
          Length = 763

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKSATTQI 8
           P S TTQ+
Sbjct: 120 PNSCTTQV 127



>XP_005257952
          Length = 763

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKSATTQI 8
           P S TTQ+
Sbjct: 120 PNSCTTQV 127



>XP_006722281
          Length = 769

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKSATTQI 8
           P S TTQ+
Sbjct: 181 PNSCTTQV 188



>XP_006722280
          Length = 799

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKSATTQI 8
           P S TTQ+
Sbjct: 181 PNSCTTQV 188



>XP_005257949
          Length = 823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKSATTQI 8
           P S TTQ+
Sbjct: 181 PNSCTTQV 188



>NP_003728
          Length = 3298

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    PKSATTQIL 9
            P+SA+ Q+L
Sbjct: 1300 PRSASLQLL 1308



>NP_061916
          Length = 666

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   PKSATTQ 7
           PK+ TTQ
Sbjct: 458 PKTGTTQ 464



>XP_005253264
          Length = 3443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    PKSATTQIL 9
            P+SA+ Q+L
Sbjct: 1445 PRSASLQLL 1453



>XP_006717514
          Length = 666

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   PKSATTQ 7
           PK+ TTQ
Sbjct: 458 PKTGTTQ 464



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.115    0.293 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 368,558
Number of extensions: 624
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)