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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              19     665
XP_006716142                                                           19     665
XP_005250088                                                           19     665
XP_006716141                                                           19     665
XP_005250085                                                           19     665
XP_006716140                                                           19     665
XP_005250084                                                           19     665
XP_005250083                                                           19     665
XP_005250082                                                           19     665
NP_997297                                                              18    1935
NP_055121                                                              16    5631
NP_001269346                                                           16    5631
NP_001035915                                                           16    5631
NP_689564                                                              16    5631
NP_001257577                                                           16    5631
NP_060009                                                              16    5631
NP_919274                                                              16    5631
XP_006721514                                                           16    5631
XP_006721513                                                           16    5631
XP_005256514                                                           16    5631
XP_006721125                                                           16    5631
XP_005267366                                                           16    5631
XP_006720083                                                           16    5631
NP_694591                                                              16    5631
NP_060350                                                              15    9605
XP_006719000                                                           15    9605
XP_005264448                                                           15    9605
XP_005264447                                                           15    9605
XP_005264446                                                           15    9605
NP_612351                                                              15    9605

>NP_733751
          Length = 4911

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   PKLVMESV 8
           PKLVMESV
Sbjct: 685 PKLVMESV 692



>XP_006716142
          Length = 4927

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   PKLVMESV 8
           PKLVMESV
Sbjct: 686 PKLVMESV 693



>XP_005250088
          Length = 4928

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   PKLVMESV 8
           PKLVMESV
Sbjct: 686 PKLVMESV 693



>XP_006716141
          Length = 4959

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   PKLVMESV 8
           PKLVMESV
Sbjct: 686 PKLVMESV 693



>XP_005250085
          Length = 4979

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   PKLVMESV 8
           PKLVMESV
Sbjct: 686 PKLVMESV 693



>XP_006716140
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   PKLVMESV 8
           PKLVMESV
Sbjct: 686 PKLVMESV 693



>XP_005250084
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   PKLVMESV 8
           PKLVMESV
Sbjct: 686 PKLVMESV 693



>XP_005250083
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   PKLVMESV 8
           PKLVMESV
Sbjct: 685 PKLVMESV 692



>XP_005250082
          Length = 4983

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   PKLVMESV 8
           PKLVMESV
Sbjct: 686 PKLVMESV 693



>NP_997297
          Length = 1318

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   PKLVMESV 8
           PKLVME++
Sbjct: 120 PKLVMETI 127



>NP_055121
          Length = 505

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   PKLVME 6
           PKLVME
Sbjct: 464 PKLVME 469



>NP_001269346
          Length = 566

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   PKLVME 6
           PKLVME
Sbjct: 287 PKLVME 292



>NP_001035915
          Length = 596

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   PKLVME 6
           PKLVME
Sbjct: 354 PKLVME 359



>NP_689564
          Length = 612

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   PKLVME 6
           PKLVME
Sbjct: 370 PKLVME 375



>NP_001257577
          Length = 569

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   PKLVME 6
           PKLVME
Sbjct: 327 PKLVME 332



>NP_060009
          Length = 4116

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    KLVMESV 8
            KLVMES+
Sbjct: 2796 KLVMESI 2802



>NP_919274
          Length = 509

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PKLVMESVA 9
           P L+ME VA
Sbjct: 323 PSLLMEDVA 331



>XP_006721514
          Length = 596

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   PKLVME 6
           PKLVME
Sbjct: 354 PKLVME 359



>XP_006721513
          Length = 598

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   PKLVME 6
           PKLVME
Sbjct: 319 PKLVME 324



>XP_005256514
          Length = 633

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   PKLVME 6
           PKLVME
Sbjct: 354 PKLVME 359



>XP_006721125
          Length = 4070

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    KLVMESV 8
            KLVMES+
Sbjct: 2750 KLVMESI 2756



>XP_005267366
          Length = 1330

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    PKLVME 6
            PKLVME
Sbjct: 1300 PKLVME 1305



>XP_006720083
          Length = 1355

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    PKLVME 6
            PKLVME
Sbjct: 1325 PKLVME 1330



>NP_694591
          Length = 1382

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    PKLVME 6
            PKLVME
Sbjct: 1352 PKLVME 1357



>NP_060350
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PKLVMESVA 9
           PK+ +ES+A
Sbjct: 474 PKVEVESIA 482



>XP_006719000
          Length = 636

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PKLVME 6
           PK+VME
Sbjct: 272 PKMVME 277



>XP_005264448
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PKLVMESVA 9
           PK+ +ES+A
Sbjct: 474 PKVEVESIA 482



>XP_005264447
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PKLVMESVA 9
           PK+ +ES+A
Sbjct: 474 PKVEVESIA 482



>XP_005264446
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PKLVMESVA 9
           PK+ +ES+A
Sbjct: 474 PKVEVESIA 482



>NP_612351
          Length = 636

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PKLVME 6
           PK+VME
Sbjct: 272 PKMVME 277



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.124    0.311 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 323,092
Number of extensions: 446
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)