Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              19     665
NP_001035957                                                           19     665
XP_006721990                                                           19     665
XP_006721989                                                           19     665
XP_005258041                                                           19     665
XP_006721988                                                           19     665
XP_006721987                                                           19     665
XP_006721986                                                           19     665
XP_005258040                                                           19     665
XP_006721985                                                           19     665
NP_055859                                                              19     869
NP_001072                                                              17    2528
NP_001269421                                                           17    3301
NP_003963                                                              17    3301
XP_005270323                                                           17    3301
XP_005270322                                                           17    3301
NP_001129335                                                           16    7355
XP_006719123                                                           16    7355
NP_001129336                                                           16    7355
NP_776163                                                              16    7355
NP_001129575                                                           15    9605
NP_597705                                                              15    9605
NP_612385                                                              15    9605
NP_001668                                                              15    9605
NP_001004485                                                           15    9605
>NP_000258
          Length = 2818

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    PKIQVLLLT 9
            PKIQ LLLT
Sbjct: 2592 PKIQALLLT 2600
>NP_001035957
          Length = 2839

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    PKIQVLLLT 9
            PKIQ LLLT
Sbjct: 2613 PKIQALLLT 2621
>XP_006721990
          Length = 2823

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    PKIQVLLLT 9
            PKIQ LLLT
Sbjct: 2623 PKIQALLLT 2631
>XP_006721989
          Length = 2823

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    PKIQVLLLT 9
            PKIQ LLLT
Sbjct: 2623 PKIQALLLT 2631
>XP_005258041
          Length = 2836

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    PKIQVLLLT 9
            PKIQ LLLT
Sbjct: 2592 PKIQALLLT 2600
>XP_006721988
          Length = 2846

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    PKIQVLLLT 9
            PKIQ LLLT
Sbjct: 2602 PKIQALLLT 2610
>XP_006721987
          Length = 2849

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    PKIQVLLLT 9
            PKIQ LLLT
Sbjct: 2623 PKIQALLLT 2631
>XP_006721986
          Length = 2854

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    PKIQVLLLT 9
            PKIQ LLLT
Sbjct: 2610 PKIQALLLT 2618
>XP_005258040
          Length = 2857

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    PKIQVLLLT 9
            PKIQ LLLT
Sbjct: 2613 PKIQALLLT 2621
>XP_006721985
          Length = 2867

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    PKIQVLLLT 9
            PKIQ LLLT
Sbjct: 2623 PKIQALLLT 2631
>NP_055859
          Length = 613

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   PKIQVLLLT 9
           P++QVLLLT
Sbjct: 512 PEVQVLLLT 520
>NP_001072
          Length = 3623

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2    KIQVLLLT 9
            K+QVLLLT
Sbjct: 1365 KLQVLLLT 1372
>NP_001269421
          Length = 230

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   PKIQVLLL 8
           PKI++LLL
Sbjct: 115 PKIKILLL 122
>NP_003963
          Length = 1849

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    PKIQVLLLT 9
            P I VLLLT
Sbjct: 1697 PSIDVLLLT 1705
>XP_005270323
          Length = 1519

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    PKIQVLLLT 9
            P I VLLLT
Sbjct: 1367 PSIDVLLLT 1375
>XP_005270322
          Length = 1646

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    PKIQVLLLT 9
            P I VLLLT
Sbjct: 1494 PSIDVLLLT 1502
>NP_001129335
          Length = 262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  PKIQVLLLT 9
          P+IQ+ LLT
Sbjct: 65 PEIQLRLLT 73
>XP_006719123
          Length = 262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  PKIQVLLLT 9
          P+IQ+ LLT
Sbjct: 65 PEIQLRLLT 73
>NP_001129336
          Length = 262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  PKIQVLLLT 9
          P+IQ+ LLT
Sbjct: 65 PEIQLRLLT 73
>NP_776163
          Length = 262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  PKIQVLLLT 9
          P+IQ+ LLT
Sbjct: 65 PEIQLRLLT 73
>NP_001129575
          Length = 1108

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PKIQVL 6
           PK+QVL
Sbjct: 145 PKVQVL 150
>NP_597705
          Length = 1099

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PKIQVL 6
           PK+QVL
Sbjct: 143 PKVQVL 148
>NP_612385
          Length = 440

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KIQVLLLT 9
          K QVL+LT
Sbjct: 67 KAQVLMLT 74
>NP_001668
          Length = 303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  IQVLLLT 9
          IQV+LLT
Sbjct: 54 IQVMLLT 60
>NP_001004485
          Length = 319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  PKIQVLL 7
          PK+QV++
Sbjct: 21 PKVQVII 27
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.148    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 410,143
Number of extensions: 612
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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