Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001264326                                                           17    2528
NP_001138486                                                           17    2528
NP_056516                                                              17    3301
NP_001120869                                                           17    3301
NP_001120870                                                           17    3301
NP_056323                                                              17    4312
NP_001004318                                                           17    4312
NP_002800                                                              17    4312
NP_001171646                                                           17    4312
NP_001171647                                                           17    4312
NP_001137310                                                           16    5631
NP_808218                                                              16    5631
NP_055947                                                              16    5631
NP_001271439                                                           16    5631
NP_001271438                                                           16    5631
XP_006721567                                                           16    5631
XP_005249729                                                           16    5631
XP_005248043                                                           16    5631
XP_006713958                                                           16    5631
NP_065824                                                              16    5631
NP_001106832                                                           16    5631
NP_057402                                                              16    7355
NP_777361                                                              16    7355
NP_733783                                                              16    7355
NP_001269093                                                           16    7355
NP_036358                                                              16    7355
NP_777362                                                              16    7355
NP_733782                                                              16    7355
NP_001269094                                                           16    7355
NP_002553                                                              16    7355
NP_055826                                                              16    7355
NP_001185879                                                           16    7355
XP_005266213                                                           16    7355
XP_005266212                                                           16    7355
XP_005266211                                                           16    7355
XP_005268810                                                           16    7355
XP_005268809                                                           16    7355
NP_036416                                                              16    7355
NP_061184                                                              15    9605
NP_001020278                                                           15    9605
NP_056655                                                              15    9605
NP_056656                                                              15    9605
NP_001102                                                              15    9605
NP_001180424                                                           15    9605
NP_005076                                                              15    9605
NP_644813                                                              15    9605
XP_005272276                                                           15    9605
XP_005272275                                                           15    9605
XP_006717355                                                           15    9605
XP_005272274                                                           15    9605
XP_005272273                                                           15    9605
XP_006711176                                                           15    9605
XP_006711175                                                           15    9605
XP_006711174                                                           15    9605
XP_006711173                                                           15    9605
XP_006711172                                                           15    9605
>NP_001264326
          Length = 287

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  PKFKFRAL 8
          PK+K RAL
Sbjct: 63 PKYKLRAL 70
>NP_001138486
          Length = 287

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  PKFKFRAL 8
          PK+K RAL
Sbjct: 63 PKYKLRAL 70
>NP_056516
          Length = 483

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PKFKFRA 7
           PK++FRA
Sbjct: 247 PKYRFRA 253
>NP_001120869
          Length = 457

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PKFKFRA 7
           PK++FRA
Sbjct: 247 PKYRFRA 253
>NP_001120870
          Length = 429

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PKFKFRA 7
           PK++FRA
Sbjct: 247 PKYRFRA 253
>NP_056323
          Length = 657

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PKFKFRALR 9
           P+FK+R L+
Sbjct: 486 PEFKYRILQ 494
>NP_001004318
          Length = 438

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KFKFRALR 9
           +F+FRAL+
Sbjct: 120 RFRFRALK 127
>NP_002800
          Length = 534

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  PKFKFRALR 9
          P+F  RALR
Sbjct: 91 PRFVLRALR 99
>NP_001171646
          Length = 657

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PKFKFRALR 9
           P+FK+R L+
Sbjct: 486 PEFKYRILQ 494
>NP_001171647
          Length = 657

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PKFKFRALR 9
           P+FK+R L+
Sbjct: 486 PEFKYRILQ 494
>NP_001137310
          Length = 522

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   PKFKFRA 7
           PKF FR+
Sbjct: 375 PKFNFRS 381
>NP_808218
          Length = 568

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   PKFKFRA 7
           PKF FR+
Sbjct: 375 PKFNFRS 381
>NP_055947
          Length = 957

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PKFKFRAL 8
           P++KFR L
Sbjct: 921 PQYKFREL 928
>NP_001271439
          Length = 525

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   PKFKFRA 7
           PKF FR+
Sbjct: 332 PKFNFRS 338
>NP_001271438
          Length = 551

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   PKFKFRA 7
           PKF FR+
Sbjct: 358 PKFNFRS 364
>XP_006721567
          Length = 531

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   PKFKFRA 7
           PKF FR+
Sbjct: 338 PKFNFRS 344
>XP_005249729
          Length = 968

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PKFKFRAL 8
           P++KFR L
Sbjct: 932 PQYKFREL 939
>XP_005248043
          Length = 669

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  PKFKFRAL 8
          PK K RAL
Sbjct: 48 PKLKLRAL 55
>XP_006713958
          Length = 693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  PKFKFRAL 8
          PK K RAL
Sbjct: 48 PKLKLRAL 55
>NP_065824
          Length = 693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  PKFKFRAL 8
          PK K RAL
Sbjct: 48 PKLKLRAL 55
>NP_001106832
          Length = 693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  PKFKFRAL 8
          PK K RAL
Sbjct: 48 PKLKLRAL 55
>NP_057402
          Length = 447

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKFKFRAL 8
           PK+ FR L
Sbjct: 257 PKYSFRRL 264
>NP_777361
          Length = 379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKFKFRAL 8
           PK+ FR L
Sbjct: 189 PKYSFRRL 196
>NP_733783
          Length = 497

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKFKFRAL 8
           PK+ FR L
Sbjct: 281 PKYSFRRL 288
>NP_001269093
          Length = 370

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKFKFRAL 8
           PK+ FR L
Sbjct: 247 PKYSFRRL 254
>NP_036358
          Length = 399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKFKFRAL 8
           PK+ FR L
Sbjct: 209 PKYSFRRL 216
>NP_777362
          Length = 404

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKFKFRAL 8
           PK+ FR L
Sbjct: 281 PKYSFRRL 288
>NP_733782
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKFKFRAL 8
           PK+ FR L
Sbjct: 281 PKYSFRRL 288
>NP_001269094
          Length = 349

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKFKFRAL 8
           PK+ FR L
Sbjct: 281 PKYSFRRL 288
>NP_002553
          Length = 595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKFKFRAL 8
           PK+ FR L
Sbjct: 271 PKYSFRRL 278
>NP_055826
          Length = 1858

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   PKFKFR 6
           P FKFR
Sbjct: 961 PSFKFR 966
>NP_001185879
          Length = 1880

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   PKFKFR 6
           P FKFR
Sbjct: 983 PSFKFR 988
>XP_005266213
          Length = 430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKFKFRAL 8
           PK+ FR L
Sbjct: 281 PKYSFRRL 288
>XP_005266212
          Length = 488

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKFKFRAL 8
           PK+ FR L
Sbjct: 281 PKYSFRRL 288
>XP_005266211
          Length = 512

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKFKFRAL 8
           PK+ FR L
Sbjct: 281 PKYSFRRL 288
>XP_005268810
          Length = 1005

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   PKFKFR 6
           PKF FR
Sbjct: 759 PKFSFR 764
>XP_005268809
          Length = 1023

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   PKFKFR 6
           PKF FR
Sbjct: 777 PKFSFR 782
>NP_036416
          Length = 1083

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   PKFKFR 6
           PKF FR
Sbjct: 837 PKFSFR 842
>NP_061184
          Length = 980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PKFKFRALR 9
           P  +FRALR
Sbjct: 642 PAREFRALR 650
>NP_001020278
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   PKFKFRAL 8
           P+FKF+ +
Sbjct: 238 PRFKFQVV 245
>NP_056655
          Length = 1200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   PKFKFRAL 8
           P+FKF+ +
Sbjct: 533 PRFKFQVV 540
>NP_056656
          Length = 1181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   PKFKFRAL 8
           P+FKF+ +
Sbjct: 533 PRFKFQVV 540
>NP_001102
          Length = 1226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   PKFKFRAL 8
           P+FKF+ +
Sbjct: 533 PRFKFQVV 540
>NP_001180424
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   PKFKFRAL 8
           P+FKF+ +
Sbjct: 238 PRFKFQVV 245
>NP_005076
          Length = 2090

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FKFRALR 9
          F+FRAL+
Sbjct: 17 FQFRALK 23
>NP_644813
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PKFKFRAL 8
           P+F FR L
Sbjct: 140 PRFLFRGL 147
>XP_005272276
          Length = 2036

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FKFRALR 9
          F+FRAL+
Sbjct: 58 FQFRALK 64
>XP_005272275
          Length = 2083

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FKFRALR 9
          F+FRAL+
Sbjct: 58 FQFRALK 64
>XP_006717355
          Length = 2120

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FKFRALR 9
          F+FRAL+
Sbjct: 58 FQFRALK 64
>XP_005272274
          Length = 2121

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FKFRALR 9
          F+FRAL+
Sbjct: 58 FQFRALK 64
>XP_005272273
          Length = 2132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FKFRALR 9
          F+FRAL+
Sbjct: 58 FQFRALK 64
>XP_006711176
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   PKFKFRAL 8
           P+FKF+ +
Sbjct: 238 PRFKFQVV 245
>XP_006711175
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   PKFKFRAL 8
           P+FKF+ +
Sbjct: 238 PRFKFQVV 245
>XP_006711174
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   PKFKFRAL 8
           P+FKF+ +
Sbjct: 238 PRFKFQVV 245
>XP_006711173
          Length = 1210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   PKFKFRAL 8
           P+FKF+ +
Sbjct: 543 PRFKFQVV 550
>XP_006711172
          Length = 1236

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   PKFKFRAL 8
           P+FKF+ +
Sbjct: 543 PRFKFQVV 550
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.344    0.154    0.487 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 407,219
Number of extensions: 511
Number of successful extensions: 56
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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