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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_073713                                                              18    1135
NP_001073936                                                           17    2528
NP_054706                                                              15    9605
NP_003364                                                              15    9605
NP_075053                                                              15    9605
XP_005270200                                                           15    9605
XP_005270199                                                           15    9605
XP_006714735                                                           15    9605
XP_005248626                                                           15    9605

>NP_073713
          Length = 985

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PIIVSAMLA 9
           PIIVS MLA
Sbjct: 548 PIIVSTMLA 556



>NP_001073936
          Length = 1848

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1    PIIVSAML 8
            P+IVSAML
Sbjct: 1615 PMIVSAML 1622



>NP_054706
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   PIIVSAM 7
           P+++SAM
Sbjct: 203 PVLISAM 209



>NP_003364
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   PIIVSAM 7
           P+++SAM
Sbjct: 203 PVLISAM 209



>NP_075053
          Length = 765

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PIIVSAML 8
           PI +SA+L
Sbjct: 308 PIFISALL 315



>XP_005270200
          Length = 1067

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   PIIVSAM 7
           P+++SAM
Sbjct: 203 PVLISAM 209



>XP_005270199
          Length = 1135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   PIIVSAM 7
           P+++SAM
Sbjct: 203 PVLISAM 209



>XP_006714735
          Length = 722

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PIIVSAML 8
           PI +SA+L
Sbjct: 308 PIFISALL 315



>XP_005248626
          Length = 724

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PIIVSAML 8
           PI +SA+L
Sbjct: 267 PIFISALL 274



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.138    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 225,353
Number of extensions: 208
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)