Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     390
XP_005262424                                                           20     390
NP_001273331                                                           17    2528
NP_000237                                                              17    2528
XP_006720946                                                           17    2528
XP_005255380                                                           17    2528
XP_006720945                                                           17    2528
XP_006720944                                                           17    2528
XP_005255378                                                           17    2528
XP_005255377                                                           17    2528
XP_006720943                                                           17    2528
NP_078966                                                              16    5631
NP_055426                                                              16    5631
XP_006723004                                                           16    5631
XP_006715629                                                           16    5631
XP_006715468                                                           16    5631
XP_005248756                                                           16    5631
XP_006715467                                                           16    5631
NP_078970                                                              16    5631
NP_149072                                                              16    7355
NP_056163                                                              16    7355
NP_001180298                                                           16    7355
NP_001180240                                                           16    7355
XP_003960836                                                           16    7355
XP_006726566                                                           16    7355
XP_003846345                                                           16    7355
XP_006717411                                                           16    7355
XP_006715838                                                           16    7355
XP_005263969                                                           16    7355
XP_005263968                                                           16    7355
XP_005263967                                                           16    7355
XP_005263966                                                           16    7355
XP_006710955                                                           16    7355
XP_003846677                                                           16    7355
XP_006710279                                                           16    7355
NP_001180241                                                           16    7355
NP_079004                                                              16    7355
NP_001180242                                                           16    7355
XP_006726844                                                           15    9605
XP_006723587                                                           15    9605
XP_006710121                                                           15    9605
>NP_722576
          Length = 3080

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    PFLGTRMTS 9
            P LGTRMTS
Sbjct: 1864 PVLGTRMTS 1872
>XP_005262424
          Length = 2875

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    PFLGTRMTS 9
            P LGTRMTS
Sbjct: 1659 PVLGTRMTS 1667
>NP_001273331
          Length = 1131

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FLGTRMT 8
           FLGTR+T
Sbjct: 836 FLGTRLT 842
>NP_000237
          Length = 1130

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FLGTRMT 8
           FLGTR+T
Sbjct: 835 FLGTRLT 841
>XP_006720946
          Length = 1081

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FLGTRMT 8
           FLGTR+T
Sbjct: 786 FLGTRLT 792
>XP_005255380
          Length = 1082

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FLGTRMT 8
           FLGTR+T
Sbjct: 787 FLGTRLT 793
>XP_006720945
          Length = 1096

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FLGTRMT 8
           FLGTR+T
Sbjct: 801 FLGTRLT 807
>XP_006720944
          Length = 1097

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FLGTRMT 8
           FLGTR+T
Sbjct: 802 FLGTRLT 808
>XP_005255378
          Length = 1145

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FLGTRMT 8
           FLGTR+T
Sbjct: 850 FLGTRLT 856
>XP_005255377
          Length = 1146

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FLGTRMT 8
           FLGTR+T
Sbjct: 851 FLGTRLT 857
>XP_006720943
          Length = 1208

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FLGTRMT 8
           FLGTR+T
Sbjct: 913 FLGTRLT 919
>NP_078966
          Length = 14507

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1     PFLGTRMT 8
             P L TRMT
Sbjct: 10395 PILDTRMT 10402
>NP_055426
          Length = 5596

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    PFLGTRM 7
            P++GTR+
Sbjct: 2004 PYMGTRL 2010
>XP_006723004
          Length = 6443

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    PFLGTRMT 8
            P L TRMT
Sbjct: 2309 PILDTRMT 2316
>XP_006715629
          Length = 1667

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   PFLGTRMT 8
           PFL  RMT
Sbjct: 511 PFLNYRMT 518
>XP_006715468
          Length = 3744

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   PFLGTRM 7
           P++GTR+
Sbjct: 152 PYMGTRL 158
>XP_005248756
          Length = 3905

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   PFLGTRM 7
           P++GTR+
Sbjct: 313 PYMGTRL 319
>XP_006715467
          Length = 5550

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    PFLGTRM 7
            P++GTR+
Sbjct: 2004 PYMGTRL 2010
>NP_078970
          Length = 1667

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   PFLGTRMT 8
           PFL  RMT
Sbjct: 511 PFLNYRMT 518
>NP_149072
          Length = 2240

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    PFLGTR 6
            PFLG+R
Sbjct: 1877 PFLGSR 1882
>NP_056163
          Length = 1883

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   PFLGTRM 7
           PFLG R+
Sbjct: 214 PFLGARV 220
>NP_001180298
          Length = 2240

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    PFLGTR 6
            PFLG+R
Sbjct: 1877 PFLGSR 1882
>NP_001180240
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PFLGTRMT 8
           PF+GT  T
Sbjct: 94  PFVGTEST 101
>XP_003960836
          Length = 219

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PFLGTR 6
           PFLGT+
Sbjct: 112 PFLGTQ 117
>XP_006726566
          Length = 963

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   PFLGTRMTS 9
           P L T MTS
Sbjct: 500 PLLSTSMTS 508
>XP_003846345
          Length = 219

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PFLGTR 6
           PFLGT+
Sbjct: 112 PFLGTQ 117
>XP_006717411
          Length = 963

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   PFLGTRMTS 9
           P L T MTS
Sbjct: 500 PLLSTSMTS 508
>XP_006715838
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PFLGTRMT 8
           PF+GT  T
Sbjct: 94  PFVGTEST 101
>XP_005263969
          Length = 1844

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   PFLGTRM 7
           PFLG R+
Sbjct: 175 PFLGARV 181
>XP_005263968
          Length = 1861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   PFLGTRM 7
           PFLG R+
Sbjct: 192 PFLGARV 198
>XP_005263967
          Length = 1899

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   PFLGTRM 7
           PFLG R+
Sbjct: 231 PFLGARV 237
>XP_005263966
          Length = 1900

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   PFLGTRM 7
           PFLG R+
Sbjct: 231 PFLGARV 237
>XP_006710955
          Length = 241

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   PFLGTRM 7
           PF GTR+
Sbjct: 185 PFQGTRL 191
>XP_003846677
          Length = 219

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PFLGTR 6
           PFLGT+
Sbjct: 112 PFLGTQ 117
>XP_006710279
          Length = 963

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   PFLGTRMTS 9
           P L T MTS
Sbjct: 500 PLLSTSMTS 508
>NP_001180241
          Length = 397

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PFLGTRMT 8
           PF+GT  T
Sbjct: 94  PFVGTEST 101
>NP_079004
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PFLGTRMT 8
           PF+GT  T
Sbjct: 94  PFVGTEST 101
>NP_001180242
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   PFLGTRMT 8
           PF+GT  T
Sbjct: 94  PFVGTEST 101
>XP_006726844
          Length = 225

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PFLGTRMTS 9
           PF G R+T+
Sbjct: 157 PFAGWRITA 165
>XP_006723587
          Length = 225

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PFLGTRMTS 9
           PF G R+T+
Sbjct: 157 PFAGWRITA 165
>XP_006710121
          Length = 225

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PFLGTRMTS 9
           PF G R+T+
Sbjct: 157 PFAGWRITA 165
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.137    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 416,262
Number of extensions: 677
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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