Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              20     509
NP_001263689                                                           20     509
NP_001119584                                                           20     509
NP_001119586                                                           20     509
NP_001263625                                                           20     509
NP_001119590                                                           20     509
NP_001263690                                                           20     509
NP_001119585                                                           20     509
NP_001263624                                                           20     509
NP_002516                                                              17    3301
NP_001095132                                                           17    3301
NP_001229290                                                           17    3301
XP_005270960                                                           17    3301
NP_067041                                                              17    4312
XP_006717271                                                           17    4312
NP_065103                                                              16    5631
NP_006695                                                              16    5631
NP_001124384                                                           16    5631
NP_073617                                                              16    5631
NP_612372                                                              16    5631
XP_006722230                                                           16    5631
XP_006722229                                                           16    5631
XP_005257838                                                           16    5631
XP_005257837                                                           16    5631
XP_005257836                                                           16    5631
XP_005257835                                                           16    5631
XP_005257834                                                           16    5631
NP_076915                                                              16    7355
NP_002445                                                              16    7355
NP_001129950                                                           16    7355
NP_037385                                                              16    7355
NP_001018067                                                           16    7355
NP_056965                                                              16    7355
NP_075463                                                              16    7355
XP_006719812                                                           16    7355
XP_006718243                                                           16    7355
XP_006718242                                                           16    7355
XP_006718241                                                           16    7355
XP_006718240                                                           16    7355
XP_006714537                                                           16    7355
NP_001073870                                                           16    7355
NP_078966                                                              15    9605
NP_001728                                                              15    9605
XP_006723004                                                           15    9605
NP_071433                                                              15    9605
>NP_000537
          Length = 393

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PALNKMFRQ 9
           PALNKMF Q
Sbjct: 128 PALNKMFCQ 136
>NP_001263689
          Length = 354

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  PALNKMFRQ 9
          PALNKMF Q
Sbjct: 89 PALNKMFCQ 97
>NP_001119584
          Length = 393

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PALNKMFRQ 9
           PALNKMF Q
Sbjct: 128 PALNKMFCQ 136
>NP_001119586
          Length = 341

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PALNKMFRQ 9
           PALNKMF Q
Sbjct: 128 PALNKMFCQ 136
>NP_001263625
          Length = 302

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  PALNKMFRQ 9
          PALNKMF Q
Sbjct: 89 PALNKMFCQ 97
>NP_001119590
          Length = 354

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  PALNKMFRQ 9
          PALNKMF Q
Sbjct: 89 PALNKMFCQ 97
>NP_001263690
          Length = 354

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  PALNKMFRQ 9
          PALNKMF Q
Sbjct: 89 PALNKMFCQ 97
>NP_001119585
          Length = 346

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PALNKMFRQ 9
           PALNKMF Q
Sbjct: 128 PALNKMFCQ 136
>NP_001263624
          Length = 307

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  PALNKMFRQ 9
          PALNKMF Q
Sbjct: 89 PALNKMFCQ 97
>NP_002516
          Length = 1219

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PALNKMFR 8
           PA NK++R
Sbjct: 491 PAFNKLYR 498
>NP_001095132
          Length = 1151

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PALNKMFR 8
           PA NK++R
Sbjct: 423 PAFNKLYR 430
>NP_001229290
          Length = 1087

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PALNKMFR 8
           PA NK++R
Sbjct: 359 PAFNKLYR 366
>XP_005270960
          Length = 914

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PALNKMFR 8
           PA NK++R
Sbjct: 186 PAFNKLYR 193
>NP_067041
          Length = 104

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  PALNKMFR 8
          P+LNK FR
Sbjct: 49 PSLNKDFR 56
>XP_006717271
          Length = 101

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  PALNKMFR 8
          P+LNK FR
Sbjct: 46 PSLNKDFR 53
>NP_065103
          Length = 396

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ALNKMFRQ 9
           A+N+ FRQ
Sbjct: 372 AMNRQFRQ 379
>NP_006695
          Length = 333

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ALNKMFRQ 9
           ALN++F+Q
Sbjct: 276 ALNRLFQQ 283
>NP_001124384
          Length = 365

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ALNKMFRQ 9
           ALN++F+Q
Sbjct: 308 ALNRLFQQ 315
>NP_073617
          Length = 391

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ALNKMF 7
           ALNKMF
Sbjct: 362 ALNKMF 367
>NP_612372
          Length = 821

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PALNKMFRQ 9
           P L+KM++Q
Sbjct: 581 PTLSKMWKQ 589
>XP_006722230
          Length = 610

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PALNKMFRQ 9
           P L+KM++Q
Sbjct: 369 PTLSKMWKQ 377
>XP_006722229
          Length = 610

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PALNKMFRQ 9
           P L+KM++Q
Sbjct: 369 PTLSKMWKQ 377
>XP_005257838
          Length = 657

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PALNKMFRQ 9
           P L+KM++Q
Sbjct: 417 PTLSKMWKQ 425
>XP_005257837
          Length = 658

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PALNKMFRQ 9
           P L+KM++Q
Sbjct: 417 PTLSKMWKQ 425
>XP_005257836
          Length = 822

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PALNKMFRQ 9
           P L+KM++Q
Sbjct: 581 PTLSKMWKQ 589
>XP_005257835
          Length = 869

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PALNKMFRQ 9
           P L+KM++Q
Sbjct: 629 PTLSKMWKQ 637
>XP_005257834
          Length = 870

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PALNKMFRQ 9
           P L+KM++Q
Sbjct: 629 PTLSKMWKQ 637
>NP_076915
          Length = 725

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   PALNKMFR 8
           PAL K FR
Sbjct: 175 PALRKHFR 182
>NP_002445
          Length = 698

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   PALNKMFR 8
           PAL K FR
Sbjct: 148 PALRKHFR 155
>NP_001129950
          Length = 227

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  PALNKMFRQ 9
          P+LNK+ +Q
Sbjct: 61 PSLNKLRKQ 69
>NP_037385
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LNKMFRQ 9
          LN+MFR+
Sbjct: 47 LNEMFRE 53
>NP_001018067
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LNKMFRQ 9
          LN+MFR+
Sbjct: 47 LNEMFRE 53
>NP_056965
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LNKMFRQ 9
          LN+MFR+
Sbjct: 47 LNEMFRE 53
>NP_075463
          Length = 3013

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    PALNKMFRQ 9
            P+LNKM  +
Sbjct: 2509 PSLNKMHHE 2517
>XP_006719812
          Length = 3019

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    PALNKMFRQ 9
            P+LNKM  +
Sbjct: 2509 PSLNKMHHE 2517
>XP_006718243
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LNKMFRQ 9
           LN+MFR+
Sbjct: 110 LNEMFRE 116
>XP_006718242
          Length = 258

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LNKMFRQ 9
           LN+MFR+
Sbjct: 110 LNEMFRE 116
>XP_006718241
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LNKMFRQ 9
          LN+MFR+
Sbjct: 47 LNEMFRE 53
>XP_006718240
          Length = 413

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LNKMFRQ 9
           LN+MFR+
Sbjct: 110 LNEMFRE 116
>XP_006714537
          Length = 480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   PALNKMFR 8
           PAL K FR
Sbjct: 175 PALRKHFR 182
>NP_001073870
          Length = 430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PALNKMFR 8
           P+LN +FR
Sbjct: 362 PSLNTVFR 369
>NP_078966
          Length = 14507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2     ALNKMFR 8
             ALN++FR
Sbjct: 14356 ALNQLFR 14362
>NP_001728
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  PALNKMFR 8
          P L +MFR
Sbjct: 56 PCLRQMFR 63
>XP_006723004
          Length = 6443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    ALNKMFR 8
            ALN++FR
Sbjct: 6292 ALNQLFR 6298
>NP_071433
          Length = 196

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  PALNKM 6
          PA+NKM
Sbjct: 76 PAVNKM 81
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.136    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 423,042
Number of extensions: 641
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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