Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000213                                                              21     175
NP_001087241                                                           21     175
XP_006714101                                                           21     175
XP_005265799                                                           21     175
XP_005265798                                                           21     175
XP_005265797                                                           21     175
NP_000931                                                              17    2528
XP_006721109                                                           17    2528
XP_005255374                                                           17    2528
XP_005255373                                                           17    2528
NP_001013009                                                           17    2528
NP_056110                                                              17    4312
XP_005258159                                                           17    4312
XP_006722384                                                           17    4312
NP_689500                                                              16    5631
NP_001106997                                                           16    5631
XP_005256385                                                           16    5631
XP_006721400                                                           16    5631
XP_005256381                                                           16    5631
NP_001240813                                                           16    7355
NP_803876                                                              16    7355
NP_001257321                                                           16    7355
NP_001257320                                                           16    7355
NP_958834                                                              16    7355
NP_004452                                                              16    7355
NP_387467                                                              16    7355
NP_079420                                                              16    7355
NP_001095876                                                           16    7355
XP_005269291                                                           16    7355
XP_005263305                                                           16    7355
XP_005263304                                                           16    7355
XP_006714389                                                           16    7355
XP_005263303                                                           16    7355
XP_005263302                                                           16    7355
NP_000531                                                              15    9605
NP_001036188                                                           15    9605
NP_002009                                                              15    9605
NP_001010870                                                           15    9605
NP_001161831                                                           15    9605
NP_055554                                                              15    9605
NP_001034589                                                           15    9605
NP_001243193                                                           15    9605
NP_001243194                                                           15    9605
XP_006723382                                                           15    9605
XP_006723381                                                           15    9605
XP_006723380                                                           15    9605
XP_005256615                                                           15    9605
XP_005256613                                                           15    9605
XP_005256612                                                           15    9605
XP_005267295                                                           15    9605
XP_005267294                                                           15    9605
XP_005267293                                                           15    9605
XP_006710423                                                           15    9605
NP_001070908                                                           15    9605
>NP_000213
          Length = 976

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PAKIFLVDR 9
           PAK+FLVDR
Sbjct: 114 PAKLFLVDR 122
>NP_001087241
          Length = 972

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PAKIFLVDR 9
           PAK+FLVDR
Sbjct: 114 PAKLFLVDR 122
>XP_006714101
          Length = 526

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PAKIFLVDR 9
           PAK+FLVDR
Sbjct: 114 PAKLFLVDR 122
>XP_005265799
          Length = 973

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PAKIFLVDR 9
           PAK+FLVDR
Sbjct: 114 PAKLFLVDR 122
>XP_005265798
          Length = 976

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PAKIFLVDR 9
           PAK+FLVDR
Sbjct: 114 PAKLFLVDR 122
>XP_005265797
          Length = 977

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PAKIFLVDR 9
           PAK+FLVDR
Sbjct: 114 PAKLFLVDR 122
>NP_000931
          Length = 354

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  PAKIFLVD 8
          P KIFL+D
Sbjct: 82 PGKIFLMD 89
>XP_006721109
          Length = 458

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAKIFLVDR 9
           P KIF +DR
Sbjct: 440 PNKIFYIDR 448
>XP_005255374
          Length = 458

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAKIFLVDR 9
           P KIF +DR
Sbjct: 440 PNKIFYIDR 448
>XP_005255373
          Length = 519

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAKIFLVDR 9
           P KIF +DR
Sbjct: 501 PNKIFYIDR 509
>NP_001013009
          Length = 458

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAKIFLVDR 9
           P KIF +DR
Sbjct: 440 PNKIFYIDR 448
>NP_056110
          Length = 2005

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    PAKIFLVD 8
            PAK+ L+D
Sbjct: 1254 PAKLLLID 1261
>XP_005258159
          Length = 1817

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    PAKIFLVD 8
            PAK+ L+D
Sbjct: 1066 PAKLLLID 1073
>XP_006722384
          Length = 1877

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    PAKIFLVD 8
            PAK+ L+D
Sbjct: 1126 PAKLLLID 1133
>NP_689500
          Length = 539

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KIFLVDR 9
           K+F++DR
Sbjct: 399 KVFMIDR 405
>NP_001106997
          Length = 614

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KIFLVDR 9
           K+F++DR
Sbjct: 474 KVFMIDR 480
>XP_005256385
          Length = 400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KIFLVDR 9
           K+F++DR
Sbjct: 260 KVFMIDR 266
>XP_006721400
          Length = 539

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KIFLVDR 9
           K+F++DR
Sbjct: 399 KVFMIDR 405
>XP_005256381
          Length = 597

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KIFLVDR 9
           K+F++DR
Sbjct: 457 KVFMIDR 463
>NP_001240813
          Length = 1166

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AKIFLVDR 9
          A +FLVDR
Sbjct: 31 AIVFLVDR 38
>NP_803876
          Length = 337

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AKIFLVDR 9
           A+IFL+D+
Sbjct: 265 ARIFLMDK 272
>NP_001257321
          Length = 325

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AKIFLVDR 9
           A+IFL+D+
Sbjct: 253 ARIFLMDK 260
>NP_001257320
          Length = 303

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AKIFLVDR 9
           A+IFL+D+
Sbjct: 231 ARIFLMDK 238
>NP_958834
          Length = 369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AKIFLVDR 9
           A+IFL+D+
Sbjct: 297 ARIFLMDK 304
>NP_004452
          Length = 508

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   PAKIFLVDR 9
           P K F +DR
Sbjct: 347 PVKYFSIDR 355
>NP_387467
          Length = 1087

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    PAKIFLV 7
            P+KIF+V
Sbjct: 1049 PSKIFMV 1055
>NP_079420
          Length = 1244

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKIFLVDR 9
           A +FLVDR
Sbjct: 109 AIVFLVDR 116
>NP_001095876
          Length = 1244

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKIFLVDR 9
           A +FLVDR
Sbjct: 109 AIVFLVDR 116
>XP_005269291
          Length = 1087

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    PAKIFLV 7
            P+KIF+V
Sbjct: 1049 PSKIFMV 1055
>XP_005263305
          Length = 1167

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AKIFLVDR 9
          A +FLVDR
Sbjct: 31 AIVFLVDR 38
>XP_005263304
          Length = 1167

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AKIFLVDR 9
          A +FLVDR
Sbjct: 31 AIVFLVDR 38
>XP_006714389
          Length = 1225

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKIFLVDR 9
           A +FLVDR
Sbjct: 109 AIVFLVDR 116
>XP_005263303
          Length = 1245

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKIFLVDR 9
           A +FLVDR
Sbjct: 109 AIVFLVDR 116
>XP_005263302
          Length = 1245

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKIFLVDR 9
           A +FLVDR
Sbjct: 109 AIVFLVDR 116
>NP_000531
          Length = 5038

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAKIFLVDR 9
           PA+  LVDR
Sbjct: 978 PAQTTLVDR 986
>NP_001036188
          Length = 5033

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAKIFLVDR 9
           PA+  LVDR
Sbjct: 978 PAQTTLVDR 986
>NP_002009
          Length = 1269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PAKIFLVDR 9
           P  +FL+DR
Sbjct: 644 PRFVFLLDR 652
>NP_001010870
          Length = 2096

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   IFLVDR 9
           +FLVDR
Sbjct: 567 VFLVDR 572
>NP_001161831
          Length = 2066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   IFLVDR 9
           +FLVDR
Sbjct: 567 VFLVDR 572
>NP_055554
          Length = 920

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AKIFLVDR 9
           +++FL+DR
Sbjct: 345 SRVFLLDR 352
>NP_001034589
          Length = 1215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   IFLVDR 9
           IFL+DR
Sbjct: 363 IFLIDR 368
>NP_001243193
          Length = 1258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PAKIFLVDR 9
           P  +FL+DR
Sbjct: 633 PRFVFLLDR 641
>NP_001243194
          Length = 1214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PAKIFLVDR 9
           P  +FL+DR
Sbjct: 589 PRFVFLLDR 597
>XP_006723382
          Length = 5027

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAKIFLVDR 9
           PA+  LVDR
Sbjct: 978 PAQTTLVDR 986
>XP_006723381
          Length = 5031

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAKIFLVDR 9
           PA+  LVDR
Sbjct: 977 PAQTTLVDR 985
>XP_006723380
          Length = 5032

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAKIFLVDR 9
           PA+  LVDR
Sbjct: 978 PAQTTLVDR 986
>XP_005256615
          Length = 1268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PAKIFLVDR 9
           P  +FL+DR
Sbjct: 643 PRFVFLLDR 651
>XP_005256613
          Length = 1295

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PAKIFLVDR 9
           P  +FL+DR
Sbjct: 643 PRFVFLLDR 651
>XP_005256612
          Length = 1296

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PAKIFLVDR 9
           P  +FL+DR
Sbjct: 644 PRFVFLLDR 652
>XP_005267295
          Length = 642

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AKIFLVDR 9
           +++FL+DR
Sbjct: 345 SRVFLLDR 352
>XP_005267294
          Length = 871

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AKIFLVDR 9
           +++FL+DR
Sbjct: 345 SRVFLLDR 352
>XP_005267293
          Length = 918

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AKIFLVDR 9
           +++FL+DR
Sbjct: 343 SRVFLLDR 350
>XP_006710423
          Length = 932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  IFLVDR 9
          IFL+DR
Sbjct: 80 IFLIDR 85
>NP_001070908
          Length = 869

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AKIFLVDR 9
           +++FL+DR
Sbjct: 343 SRVFLLDR 350
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.154    0.460 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 525,895
Number of extensions: 1060
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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