Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              20     299
XP_006716142                                                           20     299
XP_005250088                                                           20     299
XP_006716141                                                           20     299
XP_005250085                                                           20     299
XP_006716140                                                           20     299
XP_005250084                                                           20     299
XP_005250083                                                           20     299
XP_005250082                                                           20     299
NP_071351                                                              20     390
NP_001136336                                                           18    1482
XP_006721417                                                           18    1482
XP_006721416                                                           18    1482
NP_443137                                                              17    3301
XP_006712292                                                           17    3301
XP_005246323                                                           17    3301
XP_005246322                                                           17    3301
XP_006712291                                                           17    3301
XP_005246320                                                           17    3301
NP_001138355                                                           16    5631
NP_001138353                                                           16    5631
NP_694571                                                              16    5631
NP_001138354                                                           16    5631
NP_005403                                                              16    5631
NP_001159828                                                           16    5631
NP_001159829                                                           16    5631
NP_001159830                                                           16    5631
NP_001159831                                                           16    5631
NP_775834                                                              16    5631
XP_006715090                                                           16    5631
XP_006715089                                                           16    5631
XP_006715088                                                           16    5631
XP_005249008                                                           16    5631
NP_001087199                                                           16    5631
NP_057427                                                              16    7355
NP_783863                                                              16    7355
NP_001127911                                                           16    7355
NP_001127910                                                           16    7355
NP_665696                                                              16    7355
NP_944489                                                              16    7355
XP_006719749                                                           16    7355
XP_005269275                                                           16    7355
NP_001127909                                                           16    7355
NP_036546                                                              15    9605
NP_005124                                                              15    9605
NP_001274265                                                           15    9605
NP_001073153                                                           15    9605
NP_001073152                                                           15    9605
NP_003642                                                              15    9605
NP_065177                                                              15    9605
XP_005257805                                                           15    9605
XP_006722205                                                           15    9605
XP_006722204                                                           15    9605
XP_005266003                                                           15    9605
XP_005265998                                                           15    9605
NP_001139669                                                           15    9605
NP_003623                                                              15    9605
>NP_733751
          Length = 4911

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PAITERKFS 9
           PAIT+RKFS
Sbjct: 828 PAITKRKFS 836
>XP_006716142
          Length = 4927

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PAITERKFS 9
           PAIT+RKFS
Sbjct: 829 PAITKRKFS 837
>XP_005250088
          Length = 4928

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PAITERKFS 9
           PAIT+RKFS
Sbjct: 829 PAITKRKFS 837
>XP_006716141
          Length = 4959

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PAITERKFS 9
           PAIT+RKFS
Sbjct: 829 PAITKRKFS 837
>XP_005250085
          Length = 4979

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PAITERKFS 9
           PAIT+RKFS
Sbjct: 829 PAITKRKFS 837
>XP_006716140
          Length = 4982

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PAITERKFS 9
           PAIT+RKFS
Sbjct: 829 PAITKRKFS 837
>XP_005250084
          Length = 4982

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PAITERKFS 9
           PAIT+RKFS
Sbjct: 829 PAITKRKFS 837
>XP_005250083
          Length = 4982

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PAITERKFS 9
           PAIT+RKFS
Sbjct: 828 PAITKRKFS 836
>XP_005250082
          Length = 4983

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   PAITERKFS 9
           PAIT+RKFS
Sbjct: 829 PAITKRKFS 837
>NP_071351
          Length = 2752

 Score = 20.0 bits (40), Expect =   390
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    PAITERKFS 9
            P +TERKFS
Sbjct: 2279 PGVTERKFS 2287
>NP_001136336
          Length = 2521

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    PAITERKFS 9
            PA+TER F+
Sbjct: 2050 PAVTERMFN 2058
>XP_006721417
          Length = 2497

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    PAITERKFS 9
            PA+TER F+
Sbjct: 2026 PAVTERMFN 2034
>XP_006721416
          Length = 2504

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    PAITERKFS 9
            PA+TER F+
Sbjct: 2033 PAVTERMFN 2041
>NP_443137
          Length = 1092

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  PAITERKF 8
          PA+T +KF
Sbjct: 89 PAVTRKKF 96
>XP_006712292
          Length = 641

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  PAITERKF 8
          PA+T +KF
Sbjct: 89 PAVTRKKF 96
>XP_005246323
          Length = 1069

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  PAITERKF 8
          PA+T +KF
Sbjct: 89 PAVTRKKF 96
>XP_005246322
          Length = 1084

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  PAITERKF 8
          PA+T +KF
Sbjct: 89 PAVTRKKF 96
>XP_006712291
          Length = 1086

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  PAITERKF 8
          PA+T +KF
Sbjct: 89 PAVTRKKF 96
>XP_005246320
          Length = 1087

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  PAITERKF 8
          PA+T +KF
Sbjct: 89 PAVTRKKF 96
>NP_001138355
          Length = 831

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ITERKFS 9
           +TERK+S
Sbjct: 690 VTERKYS 696
>NP_001138353
          Length = 831

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ITERKFS 9
           +TERK+S
Sbjct: 690 VTERKYS 696
>NP_694571
          Length = 831

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ITERKFS 9
           +TERK+S
Sbjct: 690 VTERKYS 696
>NP_001138354
          Length = 831

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ITERKFS 9
           +TERK+S
Sbjct: 690 VTERKYS 696
>NP_005403
          Length = 504

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PAITERKF 8
           PA+T R+F
Sbjct: 429 PALTSRQF 436
>NP_001159828
          Length = 494

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PAITERKF 8
           PA+T R+F
Sbjct: 419 PALTSRQF 426
>NP_001159829
          Length = 483

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PAITERKF 8
           PA+T R+F
Sbjct: 408 PALTSRQF 415
>NP_001159830
          Length = 483

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PAITERKF 8
           PA+T R+F
Sbjct: 408 PALTSRQF 415
>NP_001159831
          Length = 483

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PAITERKF 8
           PA+T R+F
Sbjct: 408 PALTSRQF 415
>NP_775834
          Length = 508

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAITERKFS 9
           PA+TER  S
Sbjct: 315 PAVTERPSS 323
>XP_006715090
          Length = 352

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAITERKFS 9
           PA+TER  S
Sbjct: 159 PAVTERPSS 167
>XP_006715089
          Length = 445

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAITERKFS 9
           PA+TER  S
Sbjct: 252 PAVTERPSS 260
>XP_006715088
          Length = 445

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAITERKFS 9
           PA+TER  S
Sbjct: 252 PAVTERPSS 260
>XP_005249008
          Length = 499

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAITERKFS 9
           PA+TER  S
Sbjct: 306 PAVTERPSS 314
>NP_001087199
          Length = 578

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   PAITERKF 8
           PAI E KF
Sbjct: 189 PAIKEEKF 196
>NP_057427
          Length = 3114

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    PAITERKFS 9
            P++TE++ S
Sbjct: 2914 PSVTEKRLS 2922
>NP_783863
          Length = 1027

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  PAITERKF 8
          P++T +KF
Sbjct: 92 PSVTRKKF 99
>NP_001127911
          Length = 1253

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAITERKFS 9
           PA+ ERK S
Sbjct: 407 PALRERKSS 415
>NP_001127910
          Length = 1253

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAITERKFS 9
           PA+ ERK S
Sbjct: 407 PALRERKSS 415
>NP_665696
          Length = 1210

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAITERKFS 9
           PA+ ERK S
Sbjct: 407 PALRERKSS 415
>NP_944489
          Length = 976

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  PAITERKF 8
          P++T +KF
Sbjct: 92 PSVTRKKF 99
>XP_006719749
          Length = 977

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  PAITERKF 8
          P++T +KF
Sbjct: 92 PSVTRKKF 99
>XP_005269275
          Length = 1028

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  PAITERKF 8
          P++T +KF
Sbjct: 92 PSVTRKKF 99
>NP_001127909
          Length = 1237

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAITERKFS 9
           PA+ ERK S
Sbjct: 434 PALRERKSS 442
>NP_036546
          Length = 1393

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ITERKFS 9
           +TE+KFS
Sbjct: 855 MTEKKFS 861
>NP_005124
          Length = 329

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  PAITERKFS 9
          PA+ +R+F+
Sbjct: 65 PAVIKRRFT 73
>NP_001274265
          Length = 286

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  PAITERKF 8
          P I ERK+
Sbjct: 38 PVIPERKY 45
>NP_001073153
          Length = 305

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  PAITERKF 8
          P I ERK+
Sbjct: 57 PVIPERKY 64
>NP_001073152
          Length = 286

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  PAITERKF 8
          P I ERK+
Sbjct: 38 PVIPERKY 45
>NP_003642
          Length = 372

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PAITERKFS 9
           P  T+R+FS
Sbjct: 209 PPATDRQFS 217
>NP_065177
          Length = 305

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  PAITERKF 8
          P I ERK+
Sbjct: 57 PVIPERKY 64
>XP_005257805
          Length = 1308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    ITERKFS 9
            +TE+KFS
Sbjct: 1088 LTEQKFS 1094
>XP_006722205
          Length = 1384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    ITERKFS 9
            +TE+KFS
Sbjct: 1164 LTEQKFS 1170
>XP_006722204
          Length = 1384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    ITERKFS 9
            +TE+KFS
Sbjct: 1164 LTEQKFS 1170
>XP_005266003
          Length = 286

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  PAITERKF 8
          P I ERK+
Sbjct: 38 PVIPERKY 45
>XP_005265998
          Length = 286

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  PAITERKF 8
          P I ERK+
Sbjct: 38 PVIPERKY 45
>NP_001139669
          Length = 7081

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PAITERKFS 9
           P+I E K+S
Sbjct: 374 PSIVEAKYS 382
>NP_003623
          Length = 1384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    ITERKFS 9
            +TE+KFS
Sbjct: 1164 LTEQKFS 1170
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.129    0.356 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 476,208
Number of extensions: 679
Number of successful extensions: 57
Number of sequences better than 10000.0: 57
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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