Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              20     390
XP_006716142                                                           20     390
XP_005250088                                                           20     390
XP_006716141                                                           20     390
XP_005250085                                                           20     390
XP_006716140                                                           20     390
XP_005250084                                                           20     390
XP_005250083                                                           20     390
XP_005250082                                                           20     390
NP_001177652                                                           17    3301
NP_001005374                                                           17    3301
NP_612370                                                              17    3301
NP_001005373                                                           17    3301
NP_001164163                                                           17    3301
NP_776175                                                              17    3301
NP_995586                                                              17    3301
NP_001164164                                                           17    3301
NP_004901                                                              17    3301
XP_005254367                                                           17    3301
XP_006720536                                                           17    3301
XP_006717379                                                           17    3301
NP_057427                                                              16    5631
NP_001171809                                                           16    5631
NP_001098713                                                           16    5631
NP_065817                                                              16    5631
NP_001273886                                                           15    9605
NP_001243509                                                           15    9605
NP_149115                                                              15    9605
NP_004435                                                              15    9605
NP_002963                                                              15    9605
NP_001013764                                                           15    9605
NP_037438                                                              15    9605
NP_680086                                                              15    9605
XP_005261992                                                           15    9605
XP_005261991                                                           15    9605
XP_005261989                                                           15    9605
XP_005261988                                                           15    9605
XP_006719734                                                           15    9605
>NP_733751
          Length = 4911

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    PAFEAAQVK 9
            PAFEAAQV+
Sbjct: 4311 PAFEAAQVE 4319
>XP_006716142
          Length = 4927

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    PAFEAAQVK 9
            PAFEAAQV+
Sbjct: 4327 PAFEAAQVE 4335
>XP_005250088
          Length = 4928

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    PAFEAAQVK 9
            PAFEAAQV+
Sbjct: 4328 PAFEAAQVE 4336
>XP_006716141
          Length = 4959

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    PAFEAAQVK 9
            PAFEAAQV+
Sbjct: 4359 PAFEAAQVE 4367
>XP_005250085
          Length = 4979

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    PAFEAAQVK 9
            PAFEAAQV+
Sbjct: 4383 PAFEAAQVE 4391
>XP_006716140
          Length = 4982

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    PAFEAAQVK 9
            PAFEAAQV+
Sbjct: 4382 PAFEAAQVE 4390
>XP_005250084
          Length = 4982

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    PAFEAAQVK 9
            PAFEAAQV+
Sbjct: 4382 PAFEAAQVE 4390
>XP_005250083
          Length = 4982

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    PAFEAAQVK 9
            PAFEAAQV+
Sbjct: 4382 PAFEAAQVE 4390
>XP_005250082
          Length = 4983

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    PAFEAAQVK 9
            PAFEAAQV+
Sbjct: 4383 PAFEAAQVE 4391
>NP_001177652
          Length = 696

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   AFEAAQVK 9
           AFEA QVK
Sbjct: 456 AFEALQVK 463
>NP_001005374
          Length = 723

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   AFEAAQVK 9
           AFEA QVK
Sbjct: 456 AFEALQVK 463
>NP_612370
          Length = 723

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   AFEAAQVK 9
           AFEA QVK
Sbjct: 456 AFEALQVK 463
>NP_001005373
          Length = 723

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   AFEAAQVK 9
           AFEA QVK
Sbjct: 456 AFEALQVK 463
>NP_001164163
          Length = 839

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAFEAAQVK 9
           P FE AQ+K
Sbjct: 561 PKFETAQLK 569
>NP_776175
          Length = 1150

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   PAFEAAQV 8
           PAF AAQ+
Sbjct: 745 PAFTAAQI 752
>NP_995586
          Length = 641

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAFEAAQVK 9
           P FE AQ+K
Sbjct: 425 PKFETAQLK 433
>NP_001164164
          Length = 824

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   PAFEAAQVK 9
           P FE AQ+K
Sbjct: 561 PKFETAQLK 569
>NP_004901
          Length = 1244

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   PAFEAAQVK 9
           PA EAAQ++
Sbjct: 712 PALEAAQMR 720
>XP_005254367
          Length = 1031

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   PAFEAAQV 8
           PAF AAQ+
Sbjct: 626 PAFTAAQI 633
>XP_006720536
          Length = 1072

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   PAFEAAQV 8
           PAF AAQ+
Sbjct: 667 PAFTAAQI 674
>XP_006717379
          Length = 690

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   AFEAAQVK 9
           AFEA QVK
Sbjct: 456 AFEALQVK 463
>NP_057427
          Length = 3114

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AFEAAQVK 9
           AF+A+Q+K
Sbjct: 466 AFQASQIK 473
>NP_001171809
          Length = 1148

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PAFEAAQVK 9
           P+F AAQ++
Sbjct: 127 PSFPAAQIE 135
>NP_001098713
          Length = 1101

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PAFEAAQVK 9
           P+F AAQ++
Sbjct: 127 PSFPAAQIE 135
>NP_065817
          Length = 1100

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   PAFEAAQVK 9
           P+F AAQ++
Sbjct: 127 PSFPAAQIE 135
>NP_001273886
          Length = 341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AFEAAQVK 9
           AFE  QVK
Sbjct: 100 AFETLQVK 107
>NP_001243509
          Length = 178

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  PAFEAAQV 8
          PA EAA++
Sbjct: 27 PALEAARI 34
>NP_149115
          Length = 484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AFEAAQVK 9
           AFE  QVK
Sbjct: 243 AFETLQVK 250
>NP_004435
          Length = 987

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   PAFEAAQ 7
           P FEAA+
Sbjct: 257 PGFEAAE 263
>NP_002963
          Length = 1893

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FEAAQVK 9
            FEA QVK
Sbjct: 1380 FEARQVK 1386
>NP_001013764
          Length = 1271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PAFEAAQVK 9
           P FEAA ++
Sbjct: 639 PRFEAAAIQ 647
>NP_037438
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  PAFEAAQV 8
          PAF  AQV
Sbjct: 77 PAFPRAQV 84
>NP_680086
          Length = 256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  PAFEAAQV 8
          PAF  AQV
Sbjct: 77 PAFPRAQV 84
>XP_005261992
          Length = 1867

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FEAAQVK 9
            FEA QVK
Sbjct: 1354 FEARQVK 1360
>XP_005261991
          Length = 1868

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FEAAQVK 9
            FEA QVK
Sbjct: 1355 FEARQVK 1361
>XP_005261989
          Length = 1891

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FEAAQVK 9
            FEA QVK
Sbjct: 1378 FEARQVK 1384
>XP_005261988
          Length = 1894

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    FEAAQVK 9
            FEA QVK
Sbjct: 1381 FEARQVK 1387
>XP_006719734
          Length = 411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AFEAAQVK 9
           AFE  QVK
Sbjct: 243 AFETLQVK 250
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.121    0.329 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 329,702
Number of extensions: 347
Number of successful extensions: 38
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 38
Number of HSP's successfully gapped: 38
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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