Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000213                                                              18    1482
NP_001087241                                                           18    1482
XP_005265799                                                           18    1482
XP_005265798                                                           18    1482
XP_005265797                                                           18    1482
NP_996816                                                              17    4312
NP_653310                                                              16    5631
NP_065894                                                              16    5631
XP_005256076                                                           16    5631
XP_006721289                                                           16    5631
XP_006721288                                                           16    5631
XP_006721287                                                           16    5631
XP_005256073                                                           16    5631
XP_005256072                                                           16    5631
XP_005254476                                                           16    5631
XP_005254474                                                           16    5631
XP_006712015                                                           16    5631
NP_060460                                                              16    5631
NP_001091681                                                           16    7355
NP_001091683                                                           16    7355
NP_001091682                                                           16    7355
NP_000852                                                              16    7355
NP_005494                                                              16    7355
NP_001123886                                                           16    7355
NP_006071                                                              16    7355
NP_001154                                                              16    7355
NP_001265043                                                           16    7355
NP_001265042                                                           16    7355
NP_001455                                                              16    7355
XP_006725606                                                           16    7355
XP_005254388                                                           16    7355
XP_006725182                                                           16    7355
XP_006720544                                                           16    7355
XP_006720543                                                           16    7355
XP_005252025                                                           16    7355
XP_005252024                                                           16    7355
XP_006717156                                                           16    7355
XP_005273525                                                           16    7355
XP_006715902                                                           16    7355
XP_006715901                                                           16    7355
XP_005250168                                                           16    7355
XP_005250167                                                           16    7355
NP_001276793                                                           15    9605
NP_001276791                                                           15    9605
NP_001276790                                                           15    9605
NP_001276792                                                           15    9605
NP_058515                                                              15    9605
XP_005263586                                                           15    9605
XP_005263585                                                           15    9605
XP_005263584                                                           15    9605
XP_005263581                                                           15    9605
XP_005263580                                                           15    9605
XP_005263579                                                           15    9605
XP_005263578                                                           15    9605
NP_060689                                                              15    9605
>NP_000213
          Length = 976

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NYVGSTASS 9
           N VGSTASS
Sbjct: 958 NSVGSTASS 966
>NP_001087241
          Length = 972

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NYVGSTASS 9
           N VGSTASS
Sbjct: 954 NSVGSTASS 962
>XP_005265799
          Length = 973

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NYVGSTASS 9
           N VGSTASS
Sbjct: 955 NSVGSTASS 963
>XP_005265798
          Length = 976

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NYVGSTASS 9
           N VGSTASS
Sbjct: 958 NSVGSTASS 966
>XP_005265797
          Length = 977

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NYVGSTASS 9
           N VGSTASS
Sbjct: 959 NSVGSTASS 967
>NP_996816
          Length = 5202

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    NYVGSTAS 8
            N VGSTAS
Sbjct: 4913 NEVGSTAS 4920
>NP_653310
          Length = 529

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   NYVGSTAS 8
           ++VGSTAS
Sbjct: 94  SHVGSTAS 101
>NP_065894
          Length = 1400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NYVGSTASS 9
           ++ GSTASS
Sbjct: 229 HHTGSTASS 237
>XP_005256076
          Length = 607

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   YVGSTASS 9
           YVG T SS
Sbjct: 523 YVGKTTSS 530
>XP_006721289
          Length = 660

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   YVGSTASS 9
           YVG T SS
Sbjct: 576 YVGKTTSS 583
>XP_006721288
          Length = 799

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   YVGSTASS 9
           YVG T SS
Sbjct: 715 YVGKTTSS 722
>XP_006721287
          Length = 818

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   YVGSTASS 9
           YVG T SS
Sbjct: 734 YVGKTTSS 741
>XP_005256073
          Length = 879

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   YVGSTASS 9
           YVG T SS
Sbjct: 795 YVGKTTSS 802
>XP_005256072
          Length = 879

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   YVGSTASS 9
           YVG T SS
Sbjct: 795 YVGKTTSS 802
>XP_005254476
          Length = 1400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NYVGSTASS 9
           ++ GSTASS
Sbjct: 229 HHTGSTASS 237
>XP_005254474
          Length = 1406

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NYVGSTASS 9
           ++ GSTASS
Sbjct: 229 HHTGSTASS 237
>XP_006712015
          Length = 529

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   NYVGSTAS 8
           ++VGSTAS
Sbjct: 94  SHVGSTAS 101
>NP_060460
          Length = 879

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   YVGSTASS 9
           YVG T SS
Sbjct: 795 YVGKTTSS 802
>NP_001091681
          Length = 487

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NYVGSTAS 8
           +YV STAS
Sbjct: 107 DYVASTAS 114
>NP_001091683
          Length = 487

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NYVGSTAS 8
           +YV STAS
Sbjct: 107 DYVASTAS 114
>NP_001091682
          Length = 487

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NYVGSTAS 8
           +YV STAS
Sbjct: 107 DYVASTAS 114
>NP_000852
          Length = 487

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NYVGSTAS 8
           +YV STAS
Sbjct: 107 DYVASTAS 114
>NP_005494
          Length = 749

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           NY+GST
Sbjct: 375 NYLGST 380
>NP_001123886
          Length = 737

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           NY+GST
Sbjct: 375 NYLGST 380
>NP_006071
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   YVGSTA 7
           Y+GSTA
Sbjct: 502 YIGSTA 507
>NP_001154
          Length = 837

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           NY+GST
Sbjct: 464 NYLGST 469
>NP_001265043
          Length = 672

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           NYVG+T
Sbjct: 276 NYVGAT 281
>NP_001265042
          Length = 716

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           NYVG+T
Sbjct: 257 NYVGAT 262
>NP_001455
          Length = 735

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           NYVG+T
Sbjct: 276 NYVGAT 281
>XP_006725606
          Length = 737

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           NY+GST
Sbjct: 375 NYLGST 380
>XP_005254388
          Length = 749

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           NY+GST
Sbjct: 375 NYLGST 380
>XP_006725182
          Length = 705

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           NYVG+T
Sbjct: 276 NYVGAT 281
>XP_006720544
          Length = 778

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           NY+GST
Sbjct: 375 NYLGST 380
>XP_006720543
          Length = 790

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           NY+GST
Sbjct: 375 NYLGST 380
>XP_005252025
          Length = 826

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           NY+GST
Sbjct: 464 NYLGST 469
>XP_005252024
          Length = 837

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           NY+GST
Sbjct: 464 NYLGST 469
>XP_006717156
          Length = 837

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           NY+GST
Sbjct: 464 NYLGST 469
>XP_005273525
          Length = 705

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           NYVG+T
Sbjct: 276 NYVGAT 281
>XP_006715902
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   YVGSTA 7
           Y+GSTA
Sbjct: 502 YIGSTA 507
>XP_006715901
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   YVGSTA 7
           Y+GSTA
Sbjct: 502 YIGSTA 507
>XP_005250168
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   YVGSTA 7
           Y+GSTA
Sbjct: 502 YIGSTA 507
>XP_005250167
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   YVGSTA 7
           Y+GSTA
Sbjct: 502 YIGSTA 507
>NP_001276793
          Length = 890

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YVGSTASS 9
           ++G+TASS
Sbjct: 559 FLGATASS 566
>NP_001276791
          Length = 1210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YVGSTASS 9
           ++G+TASS
Sbjct: 878 FLGATASS 885
>NP_001276790
          Length = 1191

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YVGSTASS 9
           ++G+TASS
Sbjct: 878 FLGATASS 885
>NP_001276792
          Length = 1184

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YVGSTASS 9
           ++G+TASS
Sbjct: 871 FLGATASS 878
>NP_058515
          Length = 1201

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YVGSTASS 9
           ++G+TASS
Sbjct: 870 FLGATASS 877
>XP_005263586
          Length = 1036

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YVGSTASS 9
           ++G+TASS
Sbjct: 703 FLGATASS 710
>XP_005263585
          Length = 1094

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YVGSTASS 9
           ++G+TASS
Sbjct: 761 FLGATASS 768
>XP_005263584
          Length = 1095

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YVGSTASS 9
           ++G+TASS
Sbjct: 762 FLGATASS 769
>XP_005263581
          Length = 1203

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YVGSTASS 9
           ++G+TASS
Sbjct: 878 FLGATASS 885
>XP_005263580
          Length = 1204

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YVGSTASS 9
           ++G+TASS
Sbjct: 871 FLGATASS 878
>XP_005263579
          Length = 1210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YVGSTASS 9
           ++G+TASS
Sbjct: 877 FLGATASS 884
>XP_005263578
          Length = 1210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YVGSTASS 9
           ++G+TASS
Sbjct: 878 FLGATASS 885
>NP_060689
          Length = 849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NYVGST 6
           N+VGST
Sbjct: 485 NFVGST 490
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.289    0.103    0.257 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 441,396
Number of extensions: 531
Number of successful extensions: 55
Number of sequences better than 10000.0: 55
Number of HSP's gapped: 55
Number of HSP's successfully gapped: 55
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)

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