Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              20     390
NP_079484                                                              17    3301
NP_149126                                                              17    3301
NP_003276                                                              17    3301
NP_997213                                                              17    3301
NP_064504                                                              17    4312
NP_078901                                                              17    4312
XP_005250558                                                           17    4312
NP_079265                                                              16    5631
NP_001361                                                              16    5631
XP_005246923                                                           16    5631
XP_006712019                                                           16    5631
NP_001007028                                                           16    7355
NP_076984                                                              16    7355
NP_001275924                                                           16    7355
NP_001275885                                                           16    7355
NP_001275886                                                           16    7355
NP_001275881                                                           16    7355
NP_001275883                                                           16    7355
NP_001123552                                                           16    7355
NP_005523                                                              16    7355
NP_001275887                                                           16    7355
NP_653312                                                              16    7355
NP_000867                                                              16    7355
NP_001186219                                                           16    7355
NP_001073869                                                           16    7355
XP_006722933                                                           16    7355
XP_006722932                                                           16    7355
XP_006722931                                                           16    7355
XP_006722930                                                           16    7355
XP_005259697                                                           16    7355
XP_006722929                                                           16    7355
XP_005274304                                                           16    7355
XP_006716875                                                           16    7355
XP_006716874                                                           16    7355
XP_006716873                                                           16    7355
NP_733831                                                              15    9605
NP_055087                                                              15    9605
NP_001231902                                                           15    9605
NP_001231901                                                           15    9605
NP_001161419                                                           15    9605
NP_005825                                                              15    9605
NP_001348                                                              15    9605
NP_775951                                                              15    9605
NP_003864                                                              15    9605
XP_005254195                                                           15    9605
XP_005254194                                                           15    9605
XP_006717588                                                           15    9605
XP_006717587                                                           15    9605
XP_006717586                                                           15    9605
XP_006717585                                                           15    9605
XP_006717584                                                           15    9605
NP_006326                                                              15    9605
>NP_115803
          Length = 1156

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NWSALTSSA 9
           NWSAL SSA
Sbjct: 460 NWSALPSSA 468
>NP_079484
          Length = 370

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NWSALTSS 8
           NW ++TSS
Sbjct: 182 NWESVTSS 189
>NP_149126
          Length = 364

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NWSALTSS 8
           NW ++TSS
Sbjct: 176 NWESVTSS 183
>NP_003276
          Length = 1358

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    WSALTSSA 9
            WS++TS+A
Sbjct: 1117 WSSITSTA 1124
>NP_997213
          Length = 410

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   WSALTSSA 9
           WS LTS+A
Sbjct: 364 WSELTSTA 371
>NP_064504
          Length = 902

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NWSALTS 7
           NW +LTS
Sbjct: 358 NWKSLTS 364
>NP_078901
          Length = 699

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NWSALTS 7
           NW +LTS
Sbjct: 358 NWKSLTS 364
>XP_005250558
          Length = 1024

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NWSALTS 7
           NW +LTS
Sbjct: 358 NWKSLTS 364
>NP_079265
          Length = 922

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   WSALTSSA 9
           WS L SSA
Sbjct: 696 WSGLLSSA 703
>NP_001361
          Length = 4158

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    NWSALTSSA 9
            N+SA TSSA
Sbjct: 2125 NFSAQTSSA 2133
>XP_005246923
          Length = 748

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   WSALTSSA 9
           WS L SSA
Sbjct: 522 WSGLLSSA 529
>XP_006712019
          Length = 4158

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    NWSALTSSA 9
            N+SA TSSA
Sbjct: 2125 NFSAQTSSA 2133
>NP_001007028
          Length = 467

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  NWSALTSS 8
          NW A+T S
Sbjct: 42 NWLAITHS 49
>NP_076984
          Length = 526

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  NWSALTSS 8
          NW A+T S
Sbjct: 42 NWLAITHS 49
>NP_001275924
          Length = 119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3  SALTSSA 9
          SALTSSA
Sbjct: 4  SALTSSA 10
>NP_001275885
          Length = 122

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3  SALTSSA 9
          SALTSSA
Sbjct: 4  SALTSSA 10
>NP_001275886
          Length = 119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3  SALTSSA 9
          SALTSSA
Sbjct: 4  SALTSSA 10
>NP_001275881
          Length = 122

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3  SALTSSA 9
          SALTSSA
Sbjct: 4  SALTSSA 10
>NP_001275883
          Length = 119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3  SALTSSA 9
          SALTSSA
Sbjct: 4  SALTSSA 10
>NP_001123552
          Length = 122

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3  SALTSSA 9
          SALTSSA
Sbjct: 4  SALTSSA 10
>NP_005523
          Length = 119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3  SALTSSA 9
          SALTSSA
Sbjct: 4  SALTSSA 10
>NP_001275887
          Length = 119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3  SALTSSA 9
          SALTSSA
Sbjct: 4  SALTSSA 10
>NP_653312
          Length = 558

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   WSALTSS 8
           WS +TSS
Sbjct: 446 WSLITSS 452
>NP_000867
          Length = 2491

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1    NWSALTSS 8
            NW A+T +
Sbjct: 1390 NWEAITGT 1397
>NP_001186219
          Length = 558

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   WSALTSS 8
           WS +TSS
Sbjct: 446 WSLITSS 452
>NP_001073869
          Length = 1357

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    NWSALTSS 8
            +W  LTSS
Sbjct: 1049 SWGGLTSS 1056
>XP_006722933
          Length = 1240

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NWSALTSS 8
           +W  LTSS
Sbjct: 932 SWGGLTSS 939
>XP_006722932
          Length = 1306

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    NWSALTSS 8
            +W  LTSS
Sbjct: 998  SWGGLTSS 1005
>XP_006722931
          Length = 1371

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    NWSALTSS 8
            +W  LTSS
Sbjct: 1063 SWGGLTSS 1070
>XP_006722930
          Length = 1372

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    NWSALTSS 8
            +W  LTSS
Sbjct: 1064 SWGGLTSS 1071
>XP_005259697
          Length = 1372

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    NWSALTSS 8
            +W  LTSS
Sbjct: 1064 SWGGLTSS 1071
>XP_006722929
          Length = 1372

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    NWSALTSS 8
            +W  LTSS
Sbjct: 1064 SWGGLTSS 1071
>XP_005274304
          Length = 517

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  NWSALTSS 8
          NW A+T S
Sbjct: 33 NWLAITHS 40
>XP_006716875
          Length = 742

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  WSALTSSA 9
          WSA  SSA
Sbjct: 25 WSAQVSSA 32
>XP_006716874
          Length = 776

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  WSALTSSA 9
          WSA  SSA
Sbjct: 59 WSAQVSSA 66
>XP_006716873
          Length = 782

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  WSALTSSA 9
          WSA  SSA
Sbjct: 65 WSAQVSSA 72
>NP_733831
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  WSALTSS 8
          WS+ TSS
Sbjct: 13 WSSTTSS 19
>NP_055087
          Length = 1686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   NWSALTS 7
           NW  LTS
Sbjct: 775 NWENLTS 781
>NP_001231902
          Length = 916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   WSALTS 7
           WS LTS
Sbjct: 666 WSVLTS 671
>NP_001231901
          Length = 917

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   WSALTS 7
           WS LTS
Sbjct: 666 WSVLTS 671
>NP_001161419
          Length = 222

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   WSALTSSA 9
           W+++TS A
Sbjct: 140 WNSITSGA 147
>NP_005825
          Length = 172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  WSALTSSA 9
          W+++TS A
Sbjct: 90 WNSITSGA 97
>NP_001348
          Length = 1270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NWSALTSS 8
           NW+  TSS
Sbjct: 338 NWNPWTSS 345
>NP_775951
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   WSALT 6
           WSALT
Sbjct: 458 WSALT 462
>NP_003864
          Length = 923

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   WSALTS 7
           WS LTS
Sbjct: 673 WSVLTS 678
>XP_005254195
          Length = 1155

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   NWSALTS 7
           NW  LTS
Sbjct: 241 NWENLTS 247
>XP_005254194
          Length = 1689

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   NWSALTS 7
           NW  LTS
Sbjct: 775 NWENLTS 781
>XP_006717588
          Length = 888

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   WSALTS 7
           WS LTS
Sbjct: 638 WSVLTS 643
>XP_006717587
          Length = 889

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   WSALTS 7
           WS LTS
Sbjct: 638 WSVLTS 643
>XP_006717586
          Length = 906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   WSALTS 7
           WS LTS
Sbjct: 673 WSVLTS 678
>XP_006717585
          Length = 907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   WSALTS 7
           WS LTS
Sbjct: 673 WSVLTS 678
>XP_006717584
          Length = 924

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   WSALTS 7
           WS LTS
Sbjct: 673 WSVLTS 678
>NP_006326
          Length = 171

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  WSALTSSA 9
          W+++TS A
Sbjct: 90 WNSITSGA 97
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.298   0.0982    0.302 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 544,608
Number of extensions: 500
Number of successful extensions: 53
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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