Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     390
XP_005262424                                                           20     390
NP_057013                                                              17    2528
NP_660199                                                              17    2528
NP_660198                                                              17    2528
NP_001191759                                                           17    2528
NP_660200                                                              17    2528
NP_001137139                                                           16    5631
NP_067034                                                              16    5631
NP_660341                                                              16    7355
NP_055323                                                              16    7355
XP_005261394                                                           16    7355
XP_005261392                                                           16    7355
NP_689639                                                              16    7355
NP_000799                                                              15    9605
NP_000798                                                              15    9605
NP_001107647                                                           15    9605
NP_001271282                                                           15    9605
NP_001271281                                                           15    9605
NP_065826                                                              15    9605
NP_001253977                                                           15    9605
NP_000801                                                              15    9605
XP_006724874                                                           15    9605
XP_005274716                                                           15    9605
XP_005268315                                                           15    9605
XP_006720522                                                           15    9605
XP_006716762                                                           15    9605
XP_006716760                                                           15    9605
XP_006716759                                                           15    9605
XP_006716757                                                           15    9605
XP_005251165                                                           15    9605
XP_005251164                                                           15    9605
XP_005251163                                                           15    9605
XP_006716755                                                           15    9605
XP_006716754                                                           15    9605
XP_006716753                                                           15    9605
XP_005272199                                                           15    9605
XP_006714065                                                           15    9605
NP_055644                                                              15    9605
NP_001158509                                                           15    9605
>NP_722576
          Length = 3080

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NVTVFTSKK 9
            NVT FTSKK
Sbjct: 1519 NVTAFTSKK 1527
>XP_005262424
          Length = 2875

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NVTVFTSKK 9
            NVT FTSKK
Sbjct: 1314 NVTAFTSKK 1322
>NP_057013
          Length = 373

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NVTVFTSKK 9
           N+T FT+KK
Sbjct: 114 NLTFFTTKK 122
>NP_660199
          Length = 373

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NVTVFTSKK 9
           N+T FT+KK
Sbjct: 114 NLTFFTTKK 122
>NP_660198
          Length = 373

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NVTVFTSKK 9
           N+T FT+KK
Sbjct: 114 NLTFFTTKK 122
>NP_001191759
          Length = 373

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NVTVFTSKK 9
           N+T FT+KK
Sbjct: 114 NLTFFTTKK 122
>NP_660200
          Length = 373

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NVTVFTSKK 9
           N+T FT+KK
Sbjct: 114 NLTFFTTKK 122
>NP_001137139
          Length = 693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NVTVFT 6
           NVTVFT
Sbjct: 495 NVTVFT 500
>NP_067034
          Length = 693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NVTVFT 6
           NVTVFT
Sbjct: 495 NVTVFT 500
>NP_660341
          Length = 373

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NVTVFTSK 8
           N+T+FT++
Sbjct: 298 NLTIFTAR 305
>NP_055323
          Length = 190

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NVTVFTSK 8
           N+T+FT++
Sbjct: 115 NLTIFTAR 122
>XP_005261394
          Length = 202

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NVTVFTSK 8
           N+T+FT++
Sbjct: 127 NLTIFTAR 134
>XP_005261392
          Length = 217

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NVTVFTSK 8
           N+T+FT++
Sbjct: 115 NLTIFTAR 122
>NP_689639
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NVTVFTSK 8
           N+T+FT++
Sbjct: 311 NLTIFTAR 318
>NP_000799
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NVTVFT 6
          N+T+FT
Sbjct: 63 NITIFT 68
>NP_000798
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NVTVFT 6
          N+T+FT
Sbjct: 38 NITIFT 43
>NP_001107647
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NVTVFT 6
          N+T+FT
Sbjct: 38 NITIFT 43
>NP_001271282
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFTSKK 9
           +FTSKK
Sbjct: 636 IFTSKK 641
>NP_001271281
          Length = 902

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFTSKK 9
           +FTSKK
Sbjct: 872 IFTSKK 877
>NP_065826
          Length = 1013

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFTSKK 9
           +FTSKK
Sbjct: 974 IFTSKK 979
>NP_001253977
          Length = 926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFTSKK 9
           +FTSKK
Sbjct: 887 IFTSKK 892
>NP_000801
          Length = 462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NVTVFT 6
          N+T+FT
Sbjct: 45 NITIFT 50
>XP_006724874
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NVTVFT 6
          N+T+FT
Sbjct: 63 NITIFT 68
>XP_005274716
          Length = 491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NVTVFT 6
          N+T+FT
Sbjct: 63 NITIFT 68
>XP_005268315
          Length = 462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NVTVFT 6
          N+T+FT
Sbjct: 45 NITIFT 50
>XP_006720522
          Length = 462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NVTVFT 6
          N+T+FT
Sbjct: 45 NITIFT 50
>XP_006716762
          Length = 1571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VTVFTSK 8
            ++VFTSK
Sbjct: 1252 ISVFTSK 1258
>XP_006716760
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VTVFTSK 8
           ++VFTSK
Sbjct: 109 ISVFTSK 115
>XP_006716759
          Length = 444

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VTVFTSK 8
           ++VFTSK
Sbjct: 125 ISVFTSK 131
>XP_006716757
          Length = 1356

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VTVFTSK 8
            ++VFTSK
Sbjct: 1037 ISVFTSK 1043
>XP_005251165
          Length = 1532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VTVFTSK 8
            ++VFTSK
Sbjct: 1213 ISVFTSK 1219
>XP_005251164
          Length = 1552

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VTVFTSK 8
            ++VFTSK
Sbjct: 1233 ISVFTSK 1239
>XP_005251163
          Length = 1574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VTVFTSK 8
            ++VFTSK
Sbjct: 1255 ISVFTSK 1261
>XP_006716755
          Length = 1634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VTVFTSK 8
            ++VFTSK
Sbjct: 1315 ISVFTSK 1321
>XP_006716754
          Length = 1655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VTVFTSK 8
            ++VFTSK
Sbjct: 1336 ISVFTSK 1342
>XP_006716753
          Length = 1656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VTVFTSK 8
            ++VFTSK
Sbjct: 1337 ISVFTSK 1343
>XP_005272199
          Length = 1032

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VTVFTSKK 9
           VTV T+KK
Sbjct: 265 VTVVTTKK 272
>XP_006714065
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NVTVFT 6
          N+T+FT
Sbjct: 38 NITIFT 43
>NP_055644
          Length = 1031

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VTVFTSKK 9
           VTV T+KK
Sbjct: 265 VTVVTTKK 272
>NP_001158509
          Length = 462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  NVTVFT 6
          N+T+FT
Sbjct: 45 NITIFT 50
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.118    0.296 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 353,228
Number of extensions: 524
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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