Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000305                                                              20     299
XP_006717989                                                           20     299
NP_001153844                                                           17    4312
NP_113654                                                              17    4312
NP_002604                                                              17    4312
NP_001248745                                                           17    4312
NP_060341                                                              17    4312
NP_001243391                                                           17    4312
NP_001243389                                                           17    4312
NP_001243392                                                           17    4312
NP_001243385                                                           17    4312
XP_005255413                                                           17    4312
XP_006716727                                                           17    4312
XP_005251134                                                           17    4312
XP_005244820                                                           17    4312
XP_006710778                                                           17    4312
XP_005275825                                                           17    4312
XP_003960932                                                           17    4312
XP_006710060                                                           17    4312
NP_060719                                                              16    5631
NP_001258968                                                           16    5631
NP_001011649                                                           16    5631
NP_705932                                                              16    5631
NP_705934                                                              16    5631
NP_705933                                                              16    5631
XP_005267399                                                           16    5631
XP_005267398                                                           16    5631
XP_006717248                                                           16    5631
XP_006717247                                                           16    5631
XP_006717246                                                           16    5631
XP_006717245                                                           16    5631
XP_006717244                                                           16    5631
XP_006717243                                                           16    5631
XP_006717242                                                           16    5631
XP_005263378                                                           16    5631
NP_003719                                                              16    5631
NP_002184                                                              16    7355
NP_001171835                                                           16    7355
NP_116264                                                              16    7355
XP_005257804                                                           16    7355
XP_005257803                                                           16    7355
NP_653326                                                              15    9605
NP_038469                                                              15    9605
NP_001238911                                                           15    9605
NP_001238909                                                           15    9605
NP_001238908                                                           15    9605
NP_001238906                                                           15    9605
NP_002429                                                              15    9605
NP_071400                                                              15    9605
NP_001238903                                                           15    9605
NP_001238907                                                           15    9605
NP_005813                                                              15    9605
NP_001238902                                                           15    9605
NP_060024                                                              15    9605
XP_006726575                                                           15    9605
XP_006724655                                                           15    9605
XP_005269821                                                           15    9605
XP_006715011                                                           15    9605
XP_005248316                                                           15    9605
XP_006714512                                                           15    9605
XP_006714511                                                           15    9605
XP_006714510                                                           15    9605
XP_005270777                                                           15    9605
XP_006710615                                                           15    9605
XP_005270776                                                           15    9605
NP_001121687                                                           15    9605
NP_694991                                                              15    9605
>NP_000305
          Length = 403

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           N KVKLYFT
Sbjct: 340 NFKVKLYFT 348
>XP_006717989
          Length = 388

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           N KVKLYFT
Sbjct: 325 NFKVKLYFT 333
>NP_001153844
          Length = 1148

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           N+KVKL F+
Sbjct: 791 NIKVKLDFS 799
>NP_113654
          Length = 1246

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           N+KVKL F+
Sbjct: 889 NIKVKLDFS 897
>NP_002604
          Length = 556

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VKLYFT 9
           VKLYFT
Sbjct: 143 VKLYFT 148
>NP_001248745
          Length = 454

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VKLYFT 9
           VKLYFT
Sbjct: 143 VKLYFT 148
>NP_060341
          Length = 600

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           N+KV +YF+
Sbjct: 260 NLKVPIYFS 268
>NP_001243391
          Length = 502

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           N+KV +YF+
Sbjct: 162 NLKVPIYFS 170
>NP_001243389
          Length = 571

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           N+KV +YF+
Sbjct: 231 NLKVPIYFS 239
>NP_001243392
          Length = 499

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           N+KV +YF+
Sbjct: 159 NLKVPIYFS 167
>NP_001243385
          Length = 606

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           N+KV +YF+
Sbjct: 266 NLKVPIYFS 274
>XP_005255413
          Length = 529

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VKLYFT 9
           VKLYFT
Sbjct: 116 VKLYFT 121
>XP_006716727
          Length = 1139

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           N+KVKL F+
Sbjct: 782 NIKVKLDFS 790
>XP_005251134
          Length = 1148

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           N+KVKL F+
Sbjct: 791 NIKVKLDFS 799
>XP_005244820
          Length = 502

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           N+KV +YF+
Sbjct: 162 NLKVPIYFS 170
>XP_006710778
          Length = 484

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           N+KV +YF+
Sbjct: 260 NLKVPIYFS 268
>XP_005275825
          Length = 218

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VKLYFT 9
           VKLYFT
Sbjct: 116 VKLYFT 121
>XP_003960932
          Length = 396

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VKLYFT 9
           VKLYFT
Sbjct: 116 VKLYFT 121
>XP_006710060
          Length = 425

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VKLYFT 9
           VKLYFT
Sbjct: 116 VKLYFT 121
>NP_060719
          Length = 1893

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NVKVKLYF 8
          N+K+++YF
Sbjct: 76 NLKLRIYF 83
>NP_001258968
          Length = 1663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NVKVKLYF 8
          N+K+++YF
Sbjct: 76 NLKLRIYF 83
>NP_001011649
          Length = 1814

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NVKVKLYF 8
          N+K+++YF
Sbjct: 76 NLKLRIYF 83
>NP_705932
          Length = 622

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           NVK++ +FT
Sbjct: 248 NVKMQAFFT 256
>NP_705934
          Length = 558

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           NVK++ +FT
Sbjct: 184 NVKMQAFFT 192
>NP_705933
          Length = 603

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           NVK++ +FT
Sbjct: 248 NVKMQAFFT 256
>XP_005267399
          Length = 352

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           NVK++ +FT
Sbjct: 100 NVKMQAFFT 108
>XP_005267398
          Length = 622

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           NVK++ +FT
Sbjct: 248 NVKMQAFFT 256
>XP_006717248
          Length = 1664

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NVKVKLYF 8
          N+K+++YF
Sbjct: 76 NLKLRIYF 83
>XP_006717247
          Length = 1670

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NVKVKLYF 8
          N+K+++YF
Sbjct: 76 NLKLRIYF 83
>XP_006717246
          Length = 1820

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NVKVKLYF 8
          N+K+++YF
Sbjct: 76 NLKLRIYF 83
>XP_006717245
          Length = 1861

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NVKVKLYF 8
          N+K+++YF
Sbjct: 76 NLKLRIYF 83
>XP_006717244
          Length = 1867

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NVKVKLYF 8
          N+K+++YF
Sbjct: 76 NLKLRIYF 83
>XP_006717243
          Length = 1889

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NVKVKLYF 8
          N+K+++YF
Sbjct: 66 NLKLRIYF 73
>XP_006717242
          Length = 1898

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  NVKVKLYF 8
          N+K+++YF
Sbjct: 75 NLKLRIYF 82
>XP_005263378
          Length = 950

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           N+K+K+Y T
Sbjct: 495 NLKIKVYNT 503
>NP_003719
          Length = 931

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NVKVKLYFT 9
           N+K+K+Y T
Sbjct: 476 NLKIKVYNT 484
>NP_002184
          Length = 407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKVKLYF 8
           V+VK+YF
Sbjct: 197 VRVKVYF 203
>NP_001171835
          Length = 404

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NVKVKLYF 8
           N+KV  YF
Sbjct: 374 NIKVHAYF 381
>NP_116264
          Length = 436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NVKVKLYF 8
           N+KV  YF
Sbjct: 406 NIKVHAYF 413
>XP_005257804
          Length = 406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NVKVKLYF 8
           N+KV  YF
Sbjct: 376 NIKVHAYF 383
>XP_005257803
          Length = 438

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NVKVKLYF 8
           N+KV  YF
Sbjct: 408 NIKVHAYF 415
>NP_653326
          Length = 361

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VKLYFT 9
           VKLY+T
Sbjct: 164 VKLYYT 169
>NP_038469
          Length = 241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+KLYF
Sbjct: 116 KLKLYF 121
>NP_001238911
          Length = 173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+KLYF
Sbjct: 116 KLKLYF 121
>NP_001238909
          Length = 231

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+KLYF
Sbjct: 116 KLKLYF 121
>NP_001238908
          Length = 241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+KLYF
Sbjct: 116 KLKLYF 121
>NP_001238906
          Length = 241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+KLYF
Sbjct: 116 KLKLYF 121
>NP_002429
          Length = 1456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 1   NVKVKLYF 8
           ++K+++YF
Sbjct: 428 DIKIQMYF 435
>NP_071400
          Length = 693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+KLYF
Sbjct: 307 KMKLYF 312
>NP_001238903
          Length = 243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+KLYF
Sbjct: 126 KLKLYF 131
>NP_001238907
          Length = 241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+KLYF
Sbjct: 116 KLKLYF 121
>NP_005813
          Length = 197

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  KVKLYF 8
          K+KLYF
Sbjct: 80 KLKLYF 85
>NP_001238902
          Length = 243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+KLYF
Sbjct: 126 KLKLYF 131
>NP_060024
          Length = 1801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  NVKVKLYF 8
          N K+++YF
Sbjct: 24 NTKLQMYF 31
>XP_006726575
          Length = 786

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 1   NVKVKLYF 8
           ++K+++YF
Sbjct: 312 DIKIQMYF 319
>XP_006724655
          Length = 694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+KLYF
Sbjct: 307 KMKLYF 312
>XP_005269821
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+K+YF
Sbjct: 180 KIKMYF 185
>XP_006715011
          Length = 243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+KLYF
Sbjct: 126 KLKLYF 131
>XP_005248316
          Length = 370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VKLYFT 9
           VKLY+T
Sbjct: 173 VKLYYT 178
>XP_006714512
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VKLYFT 9
           VKLY+T
Sbjct: 181 VKLYYT 186
>XP_006714511
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VKLYFT 9
           VKLY+T
Sbjct: 181 VKLYYT 186
>XP_006714510
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VKLYFT 9
           VKLY+T
Sbjct: 181 VKLYYT 186
>XP_005270777
          Length = 453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+K+YF
Sbjct: 260 KIKIYF 265
>XP_006710615
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+K+YF
Sbjct: 232 KIKIYF 237
>XP_005270776
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+K+YF
Sbjct: 232 KIKIYF 237
>NP_001121687
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+K+YF
Sbjct: 205 KIKMYF 210
>NP_694991
          Length = 566

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   KVKLYF 8
           K+K+YF
Sbjct: 260 KIKIYF 265
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.141    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 424,496
Number of extensions: 635
Number of successful extensions: 67
Number of sequences better than 10000.0: 67
Number of HSP's gapped: 67
Number of HSP's successfully gapped: 67
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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