Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              20     390
XP_006715292                                                           20     390
NP_775771                                                              18    1935
XP_006720466                                                           18    1935
XP_005254230                                                           18    1935
XP_006720465                                                           18    1935
XP_005254228                                                           18    1935
NP_001108091                                                           16    7355
NP_001108092                                                           16    7355
NP_001108090                                                           16    7355
NP_002397                                                              16    7355
NP_001108089                                                           16    7355
NP_036475                                                              16    7355
XP_006720386                                                           16    7355
XP_006720385                                                           16    7355
XP_005268275                                                           16    7355
XP_006714930                                                           16    7355
XP_006714929                                                           16    7355
XP_005265973                                                           16    7355
XP_005265972                                                           16    7355
XP_006714524                                                           16    7355
XP_005248331                                                           16    7355
NP_892022                                                              16    7355
NP_055816                                                              15    9605
NP_001073276                                                           15    9605
NP_001073275                                                           15    9605
NP_055039                                                              15    9605
NP_056493                                                              15    9605
NP_036263                                                              15    9605
XP_005276461                                                           15    9605
XP_006719869                                                           15    9605
XP_006719868                                                           15    9605
XP_005266408                                                           15    9605
XP_005266407                                                           15    9605
XP_005266403                                                           15    9605
XP_006719867                                                           15    9605
XP_006719866                                                           15    9605
XP_005266405                                                           15    9605
XP_005266404                                                           15    9605
XP_006714394                                                           15    9605
XP_005263325                                                           15    9605
XP_005263324                                                           15    9605
NP_005851                                                              15    9605
>NP_115927
          Length = 1321

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NVAGFRGMV 9
           NVAGFRG V
Sbjct: 195 NVAGFRGTV 203
>XP_006715292
          Length = 759

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NVAGFRGMV 9
           NVAGFRG V
Sbjct: 182 NVAGFRGTV 190
>NP_775771
          Length = 1244

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VAGFRGMV 9
           VAGFRG V
Sbjct: 183 VAGFRGTV 190
>XP_006720466
          Length = 1175

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VAGFRGMV 9
           VAGFRG V
Sbjct: 114 VAGFRGTV 121
>XP_005254230
          Length = 1175

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VAGFRGMV 9
           VAGFRG V
Sbjct: 114 VAGFRGTV 121
>XP_006720465
          Length = 1239

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VAGFRGMV 9
           VAGFRG V
Sbjct: 178 VAGFRGTV 185
>XP_005254228
          Length = 1250

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VAGFRGMV 9
           VAGFRG V
Sbjct: 189 VAGFRGTV 196
>NP_001108091
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AGFRGMV 9
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>NP_001108092
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AGFRGMV 9
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>NP_001108090
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AGFRGMV 9
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>NP_002397
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AGFRGMV 9
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>NP_001108089
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AGFRGMV 9
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>NP_036475
          Length = 1086

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NVAGFRGMV 9
           N+AG++ +V
Sbjct: 197 NIAGYKAVV 205
>XP_006720386
          Length = 1571

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NVAGFRG 7
           NVA FRG
Sbjct: 257 NVATFRG 263
>XP_006720385
          Length = 1683

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NVAGFRG 7
           NVA FRG
Sbjct: 257 NVATFRG 263
>XP_005268275
          Length = 1684

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NVAGFRG 7
           NVA FRG
Sbjct: 261 NVATFRG 267
>XP_006714930
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AGFRGMV 9
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>XP_006714929
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AGFRGMV 9
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>XP_005265973
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AGFRGMV 9
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>XP_005265972
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AGFRGMV 9
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>XP_006714524
          Length = 955

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  NVAGFRGMV 9
          N+AG++ +V
Sbjct: 66 NIAGYKAVV 74
>XP_005248331
          Length = 1086

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NVAGFRGMV 9
           N+AG++ +V
Sbjct: 197 NIAGYKAVV 205
>NP_892022
          Length = 1086

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NVAGFRGMV 9
           N+AG++ +V
Sbjct: 197 NIAGYKAVV 205
>NP_055816
          Length = 3664

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   VAGFRG 7
           V+GFRG
Sbjct: 104 VSGFRG 109
>NP_001073276
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   VAGFRGM 8
           ++GF+GM
Sbjct: 345 ISGFQGM 351
>NP_001073275
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   VAGFRGM 8
           ++GF+GM
Sbjct: 345 ISGFQGM 351
>NP_055039
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   VAGFRGM 8
           ++GF+GM
Sbjct: 345 ISGFQGM 351
>NP_056493
          Length = 2946

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4    GFRGM 8
            GFRGM
Sbjct: 1306 GFRGM 1310
>NP_036263
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  VAGFRGMV 9
          V GF GMV
Sbjct: 12 VLGFLGMV 19
>XP_005276461
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   VAGFRGM 8
           ++GF+GM
Sbjct: 345 ISGFQGM 351
>XP_006719869
          Length = 2943

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4    GFRGM 8
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_006719868
          Length = 2938

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4    GFRGM 8
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_005266408
          Length = 1934

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4    GFRGM 8
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_005266407
          Length = 2641

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   GFRGM 8
           GFRGM
Sbjct: 980 GFRGM 984
>XP_005266403
          Length = 2941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4    GFRGM 8
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_006719867
          Length = 2961

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4    GFRGM 8
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_006719866
          Length = 2962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4    GFRGM 8
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_005266405
          Length = 2964

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4    GFRGM 8
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_005266404
          Length = 2967

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4    GFRGM 8
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_006714394
          Length = 960

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  VAGFRG 7
          V+GFRG
Sbjct: 26 VSGFRG 31
>XP_005263325
          Length = 970

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  VAGFRG 7
          V+GFRG
Sbjct: 26 VSGFRG 31
>XP_005263324
          Length = 1004

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  VAGFRG 7
          V+GFRG
Sbjct: 26 VSGFRG 31
>NP_005851
          Length = 263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NVAGFRGMV 9
          N+ GF G+V
Sbjct: 82 NLLGFLGLV 90
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.147    0.444 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 504,652
Number of extensions: 879
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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