Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              21     175
NP_612404                                                              17    2528
NP_000706                                                              17    3301
XP_005273308                                                           17    3301
XP_005273309                                                           17    3301
NP_065976                                                              17    4312
NP_004200                                                              17    4312
NP_065845                                                              17    4312
NP_002636                                                              17    4312
XP_005253037                                                           17    4312
XP_005253036                                                           17    4312
XP_005253035                                                           17    4312
XP_005253034                                                           17    4312
XP_006710856                                                           17    4312
XP_006710845                                                           17    4312
NP_666533                                                              16    5631
NP_000552                                                              16    5631
XP_005270841                                                           16    5631
XP_005270839                                                           16    5631
NP_000839                                                              16    5631
NP_000841                                                              16    5631
NP_671489                                                              16    5631
NP_001135840                                                           16    5631
NP_000842                                                              16    5631
NP_075462                                                              16    7355
NP_001166281                                                           16    7355
NP_653267                                                              16    7355
NP_077741                                                              16    7355
NP_001932                                                              16    7355
XP_006723289                                                           16    7355
XP_005257635                                                           16    7355
NP_057043                                                              15    9605
NP_001074930                                                           15    9605
NP_055982                                                              15    9605
NP_055736                                                              15    9605
NP_068817                                                              15    9605
NP_001008701                                                           15    9605
NP_001239160                                                           15    9605
NP_892019                                                              15    9605
NP_001164433                                                           15    9605
NP_001546                                                              15    9605
NP_001137484                                                           15    9605
NP_001164432                                                           15    9605
XP_006724819                                                           15    9605
XP_006724818                                                           15    9605
XP_005259876                                                           15    9605
XP_006722752                                                           15    9605
XP_005259875                                                           15    9605
XP_006722751                                                           15    9605
XP_005271911                                                           15    9605
XP_006714586                                                           15    9605
>NP_004351
          Length = 882

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  NTRFKVGTD 9
          +TRFKVGTD
Sbjct: 72 DTRFKVGTD 80
>NP_612404
          Length = 593

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  TRFKVGTD 9
          TRF  GTD
Sbjct: 86 TRFSTGTD 93
>NP_000706
          Length = 597

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  TRFKVGT 8
          TRFK GT
Sbjct: 69 TRFKTGT 75
>XP_005273308
          Length = 597

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  TRFKVGT 8
          TRFK GT
Sbjct: 69 TRFKTGT 75
>XP_005273309
          Length = 597

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  TRFKVGT 8
          TRFK GT
Sbjct: 69 TRFKTGT 75
>NP_065976
          Length = 1223

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NTRFKVGTD 9
           NT+F+  TD
Sbjct: 279 NTKFQANTD 287
>NP_004200
          Length = 229

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RFKVGTD 9
           RF++GTD
Sbjct: 170 RFRLGTD 176
>NP_065845
          Length = 1537

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTRFKVGT 8
           NTR KVG+
Sbjct: 701 NTRVKVGS 708
>NP_002636
          Length = 1686

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    NTRFKVGTD 9
            N  FKVG D
Sbjct: 1134 NVMFKVGED 1142
>XP_005253037
          Length = 1306

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NTRFKVGTD 9
           N  FKVG D
Sbjct: 754 NVMFKVGED 762
>XP_005253036
          Length = 1686

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    NTRFKVGTD 9
            N  FKVG D
Sbjct: 1134 NVMFKVGED 1142
>XP_005253035
          Length = 1686

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    NTRFKVGTD 9
            N  FKVG D
Sbjct: 1134 NVMFKVGED 1142
>XP_005253034
          Length = 1686

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    NTRFKVGTD 9
            N  FKVG D
Sbjct: 1134 NVMFKVGED 1142
>XP_006710856
          Length = 978

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NTRFKVGTD 9
          NT+F+  TD
Sbjct: 34 NTKFQANTD 42
>XP_006710845
          Length = 1492

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTRFKVGT 8
           NTR KVG+
Sbjct: 656 NTRVKVGS 663
>NP_666533
          Length = 181

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NTRFKVGTD 9
          N +FK+G D
Sbjct: 48 NEKFKLGLD 56
>NP_000552
          Length = 218

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NTRFKVGTD 9
          N +FK+G D
Sbjct: 48 NEKFKLGLD 56
>XP_005270841
          Length = 218

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NTRFKVGTD 9
          N +FK+G D
Sbjct: 48 NEKFKLGLD 56
>XP_005270839
          Length = 184

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NTRFKVGTD 9
          N +FK+G D
Sbjct: 14 NEKFKLGLD 22
>NP_000839
          Length = 218

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NTRFKVGTD 9
          N +FK+G D
Sbjct: 48 NEKFKLGLD 56
>NP_000841
          Length = 218

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NTRFKVGTD 9
          N +FK+G D
Sbjct: 48 NEKFKLGLD 56
>NP_671489
          Length = 195

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NTRFKVGTD 9
          N +FK+G D
Sbjct: 48 NEKFKLGLD 56
>NP_001135840
          Length = 191

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NTRFKVGTD 9
          N +FK+G D
Sbjct: 48 NEKFKLGLD 56
>NP_000842
          Length = 218

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NTRFKVGTD 9
          N +FK+G D
Sbjct: 48 NEKFKLGLD 56
>NP_075462
          Length = 605

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TRFKVGTD 9
           T+F VGT+
Sbjct: 331 TKFMVGTE 338
>NP_001166281
          Length = 593

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TRFKVGTD 9
           T+F VGT+
Sbjct: 331 TKFMVGTE 338
>NP_653267
          Length = 4753

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    NTRFKVGTD 9
            N+RFKV  D
Sbjct: 1285 NSRFKVMDD 1293
>NP_077741
          Length = 839

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   NTRFKVGTD 9
           N  FK+ TD
Sbjct: 401 NGHFKISTD 409
>NP_001932
          Length = 896

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   NTRFKVGTD 9
           N  FK+ TD
Sbjct: 401 NGHFKISTD 409
>XP_006723289
          Length = 416

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   TRFKVGTD 9
           TRF  GT+
Sbjct: 138 TRFSAGTE 145
>XP_005257635
          Length = 605

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TRFKVGTD 9
           T+F VGT+
Sbjct: 331 TKFMVGTE 338
>NP_057043
          Length = 296

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTRFKVGT 8
           +TR+KV T
Sbjct: 203 STRYKVST 210
>NP_001074930
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   NTRFKVGTD 9
           N +FK G D
Sbjct: 96  NQKFKCGED 104
>NP_055982
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   NTRFKVGTD 9
           N +FK G D
Sbjct: 96  NQKFKCGED 104
>NP_055736
          Length = 1469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    NTRFKVGT 8
            NTR+  GT
Sbjct: 1133 NTRYYTGT 1140
>NP_068817
          Length = 1093

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NTRFKVG 7
           NTR K+G
Sbjct: 539 NTRTKIG 545
>NP_001008701
          Length = 1474

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    NTRFKVGT 8
            NTR+  GT
Sbjct: 1138 NTRYYTGT 1145
>NP_001239160
          Length = 613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NTRFKVG 7
           NTR K+G
Sbjct: 539 NTRTKIG 545
>NP_892019
          Length = 276

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FKVGTD 9
           FKVGT+
Sbjct: 135 FKVGTE 140
>NP_001164433
          Length = 1327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    NTRFKVGTD 9
            +T FK+G D
Sbjct: 1161 STMFKLGKD 1169
>NP_001546
          Length = 1336

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    NTRFKVGTD 9
            +T FK+G D
Sbjct: 1170 STMFKLGKD 1178
>NP_001137484
          Length = 258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTRFKVGT 8
           +TR+KV T
Sbjct: 165 STRYKVST 172
>NP_001164432
          Length = 1341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    NTRFKVGTD 9
            +T FK+G D
Sbjct: 1175 STMFKLGKD 1183
>XP_006724819
          Length = 1253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    NTRFKVGTD 9
            +T FK+G D
Sbjct: 1087 STMFKLGKD 1095
>XP_006724818
          Length = 1304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    NTRFKVGTD 9
            +T FK+G D
Sbjct: 1138 STMFKLGKD 1146
>XP_005259876
          Length = 1498

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    NTRFKVGT 8
            NTR+  GT
Sbjct: 1138 NTRYYTGT 1145
>XP_006722752
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    NTRFKVGT 8
            NTR+  GT
Sbjct: 1102 NTRYYTGT 1109
>XP_005259875
          Length = 1493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    NTRFKVGT 8
            NTR+  GT
Sbjct: 1133 NTRYYTGT 1140
>XP_006722751
          Length = 1498

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    NTRFKVGT 8
            NTR+  GT
Sbjct: 1138 NTRYYTGT 1145
>XP_005271911
          Length = 613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NTRFKVG 7
           NTR K+G
Sbjct: 539 NTRTKIG 545
>XP_006714586
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NTRFKVG 7
           NTR K+G
Sbjct: 481 NTRTKIG 487
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.135    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 514,232
Number of extensions: 952
Number of successful extensions: 51
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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