Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003290                                                              20     390
XP_005272419                                                           18    1935
NP_116206                                                              17    3301
NP_778203                                                              17    3301
NP_001834                                                              17    3301
NP_001073869                                                           17    3301
NP_085046                                                              17    3301
XP_006722933                                                           17    3301
XP_006722932                                                           17    3301
XP_006722931                                                           17    3301
XP_006722930                                                           17    3301
XP_005259697                                                           17    3301
XP_006722929                                                           17    3301
XP_006719304                                                           17    3301
XP_005268708                                                           17    3301
NP_001186978                                                           17    4312
NP_001078949                                                           17    4312
NP_000313                                                              16    5631
XP_005249318                                                           16    5631
NP_001957                                                              16    7355
NP_001159887                                                           16    7355
XP_006713588                                                           16    7355
NP_001350                                                              15    9605
NP_001243556                                                           15    9605
NP_003948                                                              15    9605
NP_001243558                                                           15    9605
XP_006723741                                                           15    9605
XP_006718441                                                           15    9605
XP_005253275                                                           15    9605
XP_006718438                                                           15    9605
XP_006718437                                                           15    9605
XP_005276177                                                           15    9605
XP_002345622                                                           15    9605
NP_115806                                                              15    9605

>NP_003290
          Length = 803

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NTLGRGMTI 9
           NTLGRG TI
Sbjct: 239 NTLGRGTTI 247



>XP_005272419
          Length = 216

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NTLGRGMTI 9
           NTLGRG  +
Sbjct: 206 NTLGRGFLV 214



>NP_116206
          Length = 728

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TLGRGMT 8
           TLGRGM+
Sbjct: 719 TLGRGMS 725



>NP_778203
          Length = 1007

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NTLGRG 6
           NTLGRG
Sbjct: 674 NTLGRG 679



>NP_001834
          Length = 1018

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NTLGRG 6
           NTLGRG
Sbjct: 685 NTLGRG 690



>NP_001073869
          Length = 1357

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTLGRGMT 8
           NTLG G+T
Sbjct: 849 NTLGSGVT 856




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NTLGRGMT 8
           NT G G+T
Sbjct: 651 NTFGSGVT 658



>NP_085046
          Length = 942

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TLGRGMT 8
           TLGRGM+
Sbjct: 933 TLGRGMS 939



>XP_006722933
          Length = 1240

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTLGRGMT 8
           NTLG G+T
Sbjct: 732 NTLGSGVT 739




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NTLGRGMT 8
           NT G G+T
Sbjct: 666 NTFGSGVT 673



>XP_006722932
          Length = 1306

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTLGRGMT 8
           NTLG G+T
Sbjct: 798 NTLGSGVT 805




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NTLGRGMT 8
           NT G G+T
Sbjct: 666 NTFGSGVT 673



>XP_006722931
          Length = 1371

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTLGRGMT 8
           NTLG G+T
Sbjct: 863 NTLGSGVT 870




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NTLGRGMT 8
           NT G G+T
Sbjct: 665 NTFGSGVT 672



>XP_006722930
          Length = 1372

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTLGRGMT 8
           NTLG G+T
Sbjct: 864 NTLGSGVT 871




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NTLGRGMT 8
           NT G G+T
Sbjct: 666 NTFGSGVT 673



>XP_005259697
          Length = 1372

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTLGRGMT 8
           NTLG G+T
Sbjct: 864 NTLGSGVT 871




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NTLGRGMT 8
           NT G G+T
Sbjct: 666 NTFGSGVT 673



>XP_006722929
          Length = 1372

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTLGRGMT 8
           NTLG G+T
Sbjct: 864 NTLGSGVT 871




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NTLGRGMT 8
           NT G G+T
Sbjct: 666 NTFGSGVT 673



>XP_006719304
          Length = 1018

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NTLGRG 6
           NTLGRG
Sbjct: 685 NTLGRG 690



>XP_005268708
          Length = 1018

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NTLGRG 6
           NTLGRG
Sbjct: 685 NTLGRG 690



>NP_001186978
          Length = 2715

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NTLGRGMTI 9
           NTL RG+ I
Sbjct: 788 NTLARGLII 796



>NP_001078949
          Length = 162

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LGRGMTI 9
          LGRG+T+
Sbjct: 11 LGRGLTV 17



>NP_000313
          Length = 346

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NTLGRGM 7
           NTLG+G+
Sbjct: 128 NTLGQGL 134



>XP_005249318
          Length = 205

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NTLGRGM 7
           NTLG+G+
Sbjct: 128 NTLGQGL 134



>NP_001957
          Length = 723

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TLGRGMTI 9
           T+GRG+ I
Sbjct: 307 TMGRGIVI 314



>NP_001159887
          Length = 627

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TLGRGMTI 9
           T+GRG+ I
Sbjct: 211 TMGRGIVI 218



>XP_006713588
          Length = 515

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TLGRGMTI 9
           T+GRG+ I
Sbjct: 99  TMGRGIVI 106



>NP_001350
          Length = 335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  LGRGMT 8
          LG+GMT
Sbjct: 71 LGKGMT 76



>NP_001243556
          Length = 736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TLGRGMT 8
          TLG+G T
Sbjct: 24 TLGKGQT 30



>NP_003948
          Length = 668

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TLGRGMT 8
          TLG+G T
Sbjct: 24 TLGKGQT 30



>NP_001243558
          Length = 674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TLGRGMT 8
          TLG+G T
Sbjct: 24 TLGKGQT 30



>XP_006723741
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  TLGRGM 7
          TLGRG+
Sbjct: 38 TLGRGL 43



>XP_006718441
          Length = 690

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TLGRGMT 8
          TLG+G T
Sbjct: 24 TLGKGQT 30



>XP_005253275
          Length = 720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TLGRGMT 8
          TLG+G T
Sbjct: 24 TLGKGQT 30



>XP_006718438
          Length = 742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TLGRGMT 8
          TLG+G T
Sbjct: 24 TLGKGQT 30



>XP_006718437
          Length = 758

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TLGRGMT 8
          TLG+G T
Sbjct: 24 TLGKGQT 30



>XP_005276177
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  TLGRGM 7
          TLGRG+
Sbjct: 38 TLGRGL 43



>XP_002345622
          Length = 613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  TLGRGM 7
          TLGRG+
Sbjct: 33 TLGRGL 38



>NP_115806
          Length = 778

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TLGRGMT 8
          TLG+G T
Sbjct: 39 TLGKGQT 45



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.140    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 483,860
Number of extensions: 488
Number of successful extensions: 41
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)