Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              20     390
NP_001035957                                                           20     390
XP_006721991                                                           20     390
XP_006721990                                                           20     390
XP_006721989                                                           20     390
XP_005258041                                                           20     390
XP_006721988                                                           20     390
XP_006721987                                                           20     390
XP_006721986                                                           20     390
XP_005258040                                                           20     390
XP_006721985                                                           20     390
NP_689670                                                              17    3301
XP_006710508                                                           17    3301
XP_006710507                                                           17    3301
NP_689910                                                              17    3301
NP_001123530                                                           17    3301
NP_006840                                                              17    4312
NP_000548                                                              16    5631
NP_659436                                                              16    5631
XP_006722338                                                           16    5631
NP_001001557                                                           16    7355
NP_002729                                                              16    7355
NP_997700                                                              16    7355
NP_001153239                                                           16    7355
NP_054757                                                              16    7355
NP_056052                                                              16    7355
NP_006659                                                              16    7355
XP_005267331                                                           16    7355
XP_006715771                                                           16    7355
XP_006715770                                                           16    7355
XP_006715769                                                           16    7355
NP_777557                                                              16    7355
NP_061720                                                              16    7355
NP_000431                                                              15    9605
NP_569733                                                              15    9605
NP_904324                                                              15    9605
NP_002431                                                              15    9605
NP_057169                                                              15    9605
NP_060358                                                              15    9605
NP_542159                                                              15    9605
NP_001164266                                                           15    9605
NP_775922                                                              15    9605
XP_006721119                                                           15    9605
XP_006721118                                                           15    9605
XP_006719745                                                           15    9605
XP_006719744                                                           15    9605
XP_005254044                                                           15    9605
XP_006715527                                                           15    9605
>NP_000258
          Length = 2818

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NTISKFLDS 9
           NTISKF DS
Sbjct: 939 NTISKFFDS 947
>NP_001035957
          Length = 2839

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NTISKFLDS 9
           NTISKF DS
Sbjct: 939 NTISKFFDS 947
>XP_006721991
          Length = 1632

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NTISKFLDS 9
           NTISKF DS
Sbjct: 949 NTISKFFDS 957
>XP_006721990
          Length = 2823

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NTISKFLDS 9
           NTISKF DS
Sbjct: 949 NTISKFFDS 957
>XP_006721989
          Length = 2823

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NTISKFLDS 9
           NTISKF DS
Sbjct: 949 NTISKFFDS 957
>XP_005258041
          Length = 2836

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NTISKFLDS 9
           NTISKF DS
Sbjct: 939 NTISKFFDS 947
>XP_006721988
          Length = 2846

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NTISKFLDS 9
           NTISKF DS
Sbjct: 949 NTISKFFDS 957
>XP_006721987
          Length = 2849

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NTISKFLDS 9
           NTISKF DS
Sbjct: 949 NTISKFFDS 957
>XP_006721986
          Length = 2854

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NTISKFLDS 9
           NTISKF DS
Sbjct: 936 NTISKFFDS 944
>XP_005258040
          Length = 2857

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NTISKFLDS 9
           NTISKF DS
Sbjct: 939 NTISKFFDS 947
>XP_006721985
          Length = 2867

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NTISKFLDS 9
           NTISKF DS
Sbjct: 949 NTISKFFDS 957
>NP_689670
          Length = 424

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NTISKFLDS 9
           NT+S+ LDS
Sbjct: 139 NTLSEILDS 147
>XP_006710508
          Length = 677

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NTISKFL 7
           NT+SKFL
Sbjct: 503 NTLSKFL 509
>XP_006710507
          Length = 719

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NTISKFL 7
           NT+SKFL
Sbjct: 545 NTLSKFL 551
>NP_689910
          Length = 719

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NTISKFL 7
           NT+SKFL
Sbjct: 545 NTLSKFL 551
>NP_001123530
          Length = 717

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NTISKFL 7
           NT+SKFL
Sbjct: 545 NTLSKFL 551
>NP_006840
          Length = 525

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TISKFLDS 9
           T SKFLD+
Sbjct: 486 TFSKFLDN 493
>NP_000548
          Length = 501

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTISKFLD 8
           NTI+ F+D
Sbjct: 200 NTITSFID 207
>NP_659436
          Length = 670

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  NTISKFLD 8
          NT+ +FLD
Sbjct: 85 NTVLRFLD 92
>XP_006722338
          Length = 473

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  NTISKFLD 8
          NT+ +FLD
Sbjct: 85 NTVLRFLD 92
>NP_001001557
          Length = 455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTISKFLD 8
           NTI+ F+D
Sbjct: 125 NTITSFVD 132
>NP_002729
          Length = 673

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NTISKF 6
           NT+SKF
Sbjct: 323 NTVSKF 328
>NP_997700
          Length = 671

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NTISKF 6
           NT+SKF
Sbjct: 323 NTVSKF 328
>NP_001153239
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  ISKFLDS 9
          ++KFLDS
Sbjct: 48 VAKFLDS 54
>NP_054757
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  ISKFLDS 9
          ++KFLDS
Sbjct: 48 VAKFLDS 54
>NP_056052
          Length = 1354

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    SKFLDS 9
            SKFLDS
Sbjct: 1132 SKFLDS 1137
>NP_006659
          Length = 500

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NTISKFL 7
           NT++KFL
Sbjct: 227 NTLAKFL 233
>XP_005267331
          Length = 355

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  NTISKFL 7
          NT++KFL
Sbjct: 82 NTLAKFL 88
>XP_006715771
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  ISKFLDS 9
          ++KFLDS
Sbjct: 48 VAKFLDS 54
>XP_006715770
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  ISKFLDS 9
          ++KFLDS
Sbjct: 48 VAKFLDS 54
>XP_006715769
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  ISKFLDS 9
          ++KFLDS
Sbjct: 48 VAKFLDS 54
>NP_777557
          Length = 453

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ISKFLDS 9
           ++KFLDS
Sbjct: 157 VNKFLDS 163
>NP_061720
          Length = 4024

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTISKFLD 8
           +T+S FLD
Sbjct: 871 STVSSFLD 878
>NP_000431
          Length = 860

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  ISKFLDS 9
          + KFLDS
Sbjct: 9  VEKFLDS 15
>NP_569733
          Length = 1068

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TISKFLDS 9
           TIS FL+S
Sbjct: 562 TISSFLNS 569
>NP_904324
          Length = 1068

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TISKFLDS 9
           TIS FL+S
Sbjct: 562 TISSFLNS 569
>NP_002431
          Length = 936

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NTISKFLDS 9
           + IS+FLD+
Sbjct: 387 SVISRFLDT 395
>NP_057169
          Length = 171

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  NTISKFLDS 9
          NTI+ ++D+
Sbjct: 80 NTITSYIDN 88
>NP_060358
          Length = 579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NTISKF 6
           NT+SKF
Sbjct: 307 NTLSKF 312
>NP_542159
          Length = 284

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NTISKFLDS 9
           NTI+ ++D+
Sbjct: 193 NTITSYIDN 201
>NP_001164266
          Length = 229

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NTISKFLDS 9
           NTI+ ++D+
Sbjct: 138 NTITSYIDN 146
>NP_775922
          Length = 6996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    TISKFLDS 9
            T+SK +DS
Sbjct: 4131 TVSKIVDS 4138
>XP_006721119
          Length = 582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NTISKF 6
           NT+SKF
Sbjct: 307 NTLSKF 312
>XP_006721118
          Length = 582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NTISKF 6
           NT+SKF
Sbjct: 307 NTLSKF 312
>XP_006719745
          Length = 1043

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TISKFLDS 9
           TIS FL+S
Sbjct: 562 TISSFLNS 569
>XP_006719744
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TISKFLDS 9
           TIS FL+S
Sbjct: 562 TISSFLNS 569
>XP_005254044
          Length = 1068

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TISKFLDS 9
           TIS FL+S
Sbjct: 562 TISSFLNS 569
>XP_006715527
          Length = 280

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NTISKFLD 8
           +TIS+FL+
Sbjct: 266 STISRFLE 273
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.127    0.325 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 380,467
Number of extensions: 463
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package