Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_060695                                                              17    3301
XP_005251560                                                           17    3301
NP_542141                                                              17    4312
XP_005260338                                                           17    4312
NP_001188333                                                           16    5631
NP_008921                                                              16    5631
NP_003325                                                              16    7355
NP_001129074                                                           16    7355
NP_695012                                                              16    7355
NP_001073279                                                           16    7355
NP_000395                                                              16    7355
NP_001098677                                                           16    7355
NP_002281                                                              16    7355
NP_000928                                                              16    7355
NP_001098676                                                           16    7355
NP_056288                                                              16    7355
NP_001138633                                                           16    7355
XP_005272707                                                           16    7355
XP_005272706                                                           16    7355
XP_005272705                                                           16    7355
XP_006715544                                                           16    7355
XP_005267041                                                           16    7355
XP_006715543                                                           16    7355
XP_005267040                                                           16    7355
XP_005248304                                                           16    7355
NP_001138389                                                           15    9605
NP_001138390                                                           15    9605
NP_001138386                                                           15    9605
NP_001138387                                                           15    9605
NP_000132                                                              15    9605
NP_075259                                                              15    9605
NP_001138388                                                           15    9605
NP_001138391                                                           15    9605
NP_001138385                                                           15    9605
NP_075418                                                              15    9605
NP_001156685                                                           15    9605
NP_000133                                                              15    9605
NP_075254                                                              15    9605
XP_006717776                                                           15    9605
XP_006717775                                                           15    9605
XP_006717774                                                           15    9605
XP_006717773                                                           15    9605
XP_006717772                                                           15    9605
XP_006717771                                                           15    9605
XP_006713936                                                           15    9605
XP_006713935                                                           15    9605
XP_006713934                                                           15    9605
XP_006713933                                                           15    9605
XP_006713932                                                           15    9605
XP_006713931                                                           15    9605
>NP_060695
          Length = 513

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NTFKSLVSS 9
           NTFKSL S+
Sbjct: 385 NTFKSLGST 393
>XP_005251560
          Length = 462

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NTFKSLVSS 9
           NTFKSL S+
Sbjct: 334 NTFKSLGST 342
>NP_542141
          Length = 249

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   NTFKSLVSS 9
           NT KS VSS
Sbjct: 202 NTLKSTVSS 210
>XP_005260338
          Length = 249

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   NTFKSLVSS 9
           NT KS VSS
Sbjct: 202 NTLKSTVSS 210
>NP_001188333
          Length = 281

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  NTFKSLVSS 9
          NTF S VSS
Sbjct: 59 NTFASRVSS 67
>NP_008921
          Length = 498

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  NTFKSLVSS 9
          NTF S VSS
Sbjct: 59 NTFASRVSS 67
>NP_003325
          Length = 1058

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TFKSLVSS 9
           +FKSLV+S
Sbjct: 302 SFKSLVAS 309
>NP_001129074
          Length = 546

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NTFKSLVSS 9
           N FK LVS+
Sbjct: 359 NDFKGLVSN 367
>NP_695012
          Length = 1058

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TFKSLVSS 9
           +FKSLV+S
Sbjct: 302 SFKSLVAS 309
>NP_001073279
          Length = 647

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NTFKSLVSS 9
           N FK LVS+
Sbjct: 460 NDFKGLVSN 468
>NP_000395
          Length = 677

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NTFKSLVSS 9
           N FK LVS+
Sbjct: 490 NDFKGLVSN 498
>NP_001098677
          Length = 1816

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTFKSLVS 8
           NT KSL+S
Sbjct: 337 NTMKSLLS 344
>NP_002281
          Length = 1816

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTFKSLVS 8
           NT KSL+S
Sbjct: 337 NTMKSLLS 344
>NP_000928
          Length = 1970

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NTFKSLVSS 9
           N FKS+V S
Sbjct: 764 NNFKSMVVS 772
>NP_001098676
          Length = 1823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTFKSLVS 8
           NT KSL+S
Sbjct: 344 NTMKSLLS 351
>NP_056288
          Length = 2046

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NTFKSLVSS 9
            + FKSLV+S
Sbjct: 1993 SVFKSLVAS 2001
>NP_001138633
          Length = 161

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TFKSLVSS 9
           TFKSLV +
Sbjct: 140 TFKSLVKT 147
>XP_005272707
          Length = 1058

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TFKSLVSS 9
           +FKSLV+S
Sbjct: 302 SFKSLVAS 309
>XP_005272706
          Length = 1064

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TFKSLVSS 9
           +FKSLV+S
Sbjct: 308 SFKSLVAS 315
>XP_005272705
          Length = 1109

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TFKSLVSS 9
           +FKSLV+S
Sbjct: 353 SFKSLVAS 360
>XP_006715544
          Length = 1810

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTFKSLVS 8
           NT KSL+S
Sbjct: 331 NTMKSLLS 338
>XP_005267041
          Length = 1823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTFKSLVS 8
           NT KSL+S
Sbjct: 344 NTMKSLLS 351
>XP_006715543
          Length = 1823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTFKSLVS 8
           NT KSL+S
Sbjct: 344 NTMKSLLS 351
>XP_005267040
          Length = 1823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NTFKSLVS 8
           NT KSL+S
Sbjct: 344 NTMKSLLS 351
>XP_005248304
          Length = 267

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TFKSLVSS 9
           TFKSLV +
Sbjct: 246 TFKSLVKT 253
>NP_001138389
          Length = 705

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 483 TFKDLVS 489
>NP_001138390
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 482 TFKDLVS 488
>NP_001138386
          Length = 709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 487 TFKDLVS 493
>NP_001138387
          Length = 707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 510 TFKDLVS 516
>NP_000132
          Length = 821

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 599 TFKDLVS 605
>NP_075259
          Length = 822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 600 TFKDLVS 606
>NP_001138388
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 484 TFKDLVS 490
>NP_001138391
          Length = 680

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 511 TFKDLVS 517
>NP_001138385
          Length = 769

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 600 TFKDLVS 606
>NP_075418
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 510 TFKDLVS 516
>NP_001156685
          Length = 808

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 592 TFKDLVS 598
>NP_000133
          Length = 806

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 590 TFKDLVS 596
>NP_075254
          Length = 694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 478 TFKDLVS 484
>XP_006717776
          Length = 806

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 618 TFKDLVS 624
>XP_006717775
          Length = 726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 504 TFKDLVS 510
>XP_006717774
          Length = 752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 530 TFKDLVS 536
>XP_006717773
          Length = 807

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 619 TFKDLVS 625
>XP_006717772
          Length = 838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 616 TFKDLVS 622
>XP_006717771
          Length = 839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 617 TFKDLVS 623
>XP_006713936
          Length = 807

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 590 TFKDLVS 596
>XP_006713935
          Length = 807

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 591 TFKDLVS 597
>XP_006713934
          Length = 809

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 592 TFKDLVS 598
>XP_006713933
          Length = 810

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 593 TFKDLVS 599
>XP_006713932
          Length = 810

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 594 TFKDLVS 600
>XP_006713931
          Length = 811

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TFKSLVS 8
           TFK LVS
Sbjct: 594 TFKDLVS 600
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.112    0.265 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 385,098
Number of extensions: 815
Number of successful extensions: 50
Number of sequences better than 10000.0: 50
Number of HSP's gapped: 50
Number of HSP's successfully gapped: 50
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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