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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_073713                                                              19     665
NP_671492                                                              17    2528
NP_001004065                                                           17    2528
NP_001185585                                                           17    2528
NP_009134                                                              17    2528
NP_001130034                                                           17    2528
XP_005277055                                                           16    5631
XP_006716116                                                           16    5631

>NP_073713
          Length = 985

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NSRQAVMSG 9
           +SRQAVMSG
Sbjct: 93  DSRQAVMSG 101



>NP_671492
          Length = 1090

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   NSRQAVMSG 9
           NSRQAV  G
Sbjct: 566 NSRQAVAKG 574



>NP_001004065
          Length = 948

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   NSRQAVMSG 9
           NSRQAV  G
Sbjct: 424 NSRQAVAKG 432



>NP_001185585
          Length = 961

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   NSRQAVMSG 9
           NSRQAV  G
Sbjct: 424 NSRQAVAKG 432



>NP_009134
          Length = 1103

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   NSRQAVMSG 9
           NSRQAV  G
Sbjct: 566 NSRQAVAKG 574



>NP_001130034
          Length = 859

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   NSRQAVMSG 9
           NSRQAV  G
Sbjct: 335 NSRQAVAKG 343



>XP_005277055
          Length = 246

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NSRQAVMSG 9
           NSR++++SG
Sbjct: 134 NSRESLISG 142



>XP_006716116
          Length = 246

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NSRQAVMSG 9
           NSR++++SG
Sbjct: 134 NSRESLISG 142



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.111    0.273 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 255,226
Number of extensions: 265
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)