Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002515                                                              20     299
NP_789765                                                              17    4312
NP_001123914                                                           17    4312
NP_005334                                                              17    4312
NP_004976                                                              17    4312
NP_203524                                                              17    4312
NP_001230814                                                           17    4312
NP_001230806                                                           17    4312
NP_005740                                                              17    4312
NP_060646                                                              17    4312
XP_006725199                                                           17    4312
XP_006719132                                                           17    4312
XP_006718280                                                           17    4312
NP_001258754                                                           16    5631
NP_109591                                                              16    5631
NP_001258753                                                           16    5631
NP_004559                                                              16    5631
NP_001182220                                                           16    5631
NP_001258752                                                           16    5631
NP_001258751                                                           16    5631
NP_004146                                                              16    5631
XP_005249241                                                           16    5631
XP_005249240                                                           16    5631
XP_006715176                                                           16    5631
XP_006715175                                                           16    5631
XP_005249238                                                           16    5631
XP_006715071                                                           16    5631
XP_005264132                                                           16    5631
NP_056988                                                              16    5631
NP_003931                                                              16    7355
NP_149037                                                              16    7355
NP_001182163                                                           16    7355
NP_001098992                                                           16    7355
NP_001193556                                                           16    7355
NP_065091                                                              16    7355
NP_001193555                                                           16    7355
NP_001171700                                                           16    7355
XP_006710017                                                           16    7355
XP_006710016                                                           16    7355
XP_005275771                                                           16    7355
XP_005275770                                                           16    7355
NP_001053                                                              16    7355
NP_003115                                                              15    9605
NP_110388                                                              15    9605
NP_003030                                                              15    9605
NP_001578                                                              15    9605
NP_000267                                                              15    9605
NP_005497                                                              15    9605
NP_001191184                                                           15    9605
NP_997303                                                              15    9605
NP_005015                                                              15    9605
XP_005262479                                                           15    9605
XP_005262478                                                           15    9605
XP_006724824                                                           15    9605
XP_006724823                                                           15    9605
XP_006710899                                                           15    9605
XP_005263548                                                           15    9605
XP_005245954                                                           15    9605
>NP_002515
          Length = 189

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  NSKSFVDIN 9
          NSKSF DIN
Sbjct: 86 NSKSFADIN 94
>NP_789765
          Length = 170

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  NSKSFVDIN 9
          N+KSF DI+
Sbjct: 86 NTKSFEDIH 94
>NP_001123914
          Length = 189

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  NSKSFVDIN 9
          N+KSF DI+
Sbjct: 86 NTKSFEDIH 94
>NP_005334
          Length = 189

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  NSKSFVDIN 9
          N+KSF DI+
Sbjct: 86 NTKSFEDIH 94
>NP_004976
          Length = 188

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  NSKSFVDIN 9
          N+KSF DI+
Sbjct: 86 NTKSFEDIH 94
>NP_203524
          Length = 189

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  NSKSFVDIN 9
          N+KSF DI+
Sbjct: 86 NTKSFEDIH 94
>NP_001230814
          Length = 206

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NSKSFVD 7
           N KSFVD
Sbjct: 178 NEKSFVD 184
>NP_001230806
          Length = 345

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NSKSFVD 7
           N KSFVD
Sbjct: 317 NEKSFVD 323
>NP_005740
          Length = 345

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NSKSFVD 7
           N KSFVD
Sbjct: 317 NEKSFVD 323
>NP_060646
          Length = 545

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NSKSFVDI 8
           NSK FV+I
Sbjct: 449 NSKQFVEI 456
>XP_006725199
          Length = 170

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  NSKSFVDIN 9
          N+KSF DI+
Sbjct: 86 NTKSFEDIH 94
>XP_006719132
          Length = 189

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  NSKSFVDIN 9
          N+KSF DI+
Sbjct: 86 NTKSFEDIH 94
>XP_006718280
          Length = 170

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  NSKSFVDIN 9
          N+KSF DI+
Sbjct: 86 NTKSFEDIH 94
>NP_001258754
          Length = 376

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           KSFV++N
Sbjct: 318 KSFVEVN 324
>NP_109591
          Length = 379

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           KSFV++N
Sbjct: 321 KSFVEVN 327
>NP_001258753
          Length = 376

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           KSFV++N
Sbjct: 318 KSFVEVN 324
>NP_004559
          Length = 376

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           KSFV++N
Sbjct: 318 KSFVEVN 324
>NP_001182220
          Length = 380

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           KSFV++N
Sbjct: 322 KSFVEVN 328
>NP_001258752
          Length = 395

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           KSFV++N
Sbjct: 337 KSFVEVN 343
>NP_001258751
          Length = 390

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           KSFV++N
Sbjct: 332 KSFVEVN 338
>NP_004146
          Length = 376

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           KSFV++N
Sbjct: 317 KSFVEVN 323
>XP_005249241
          Length = 376

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           KSFV++N
Sbjct: 317 KSFVEVN 323
>XP_005249240
          Length = 287

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           KSFV++N
Sbjct: 229 KSFVEVN 235
>XP_006715176
          Length = 380

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           KSFV++N
Sbjct: 322 KSFVEVN 328
>XP_006715175
          Length = 380

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           KSFV++N
Sbjct: 322 KSFVEVN 328
>XP_005249238
          Length = 409

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           KSFV++N
Sbjct: 351 KSFVEVN 357
>XP_006715071
          Length = 379

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           KSFV++N
Sbjct: 321 KSFVEVN 327
>XP_005264132
          Length = 1219

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    SKSFVDI 8
            SK+FVDI
Sbjct: 1128 SKNFVDI 1134
>NP_056988
          Length = 1220

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    SKSFVDI 8
            SK+FVDI
Sbjct: 1129 SKNFVDI 1135
>NP_003931
          Length = 863

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NSKSFVDIN 9
           NSK F+D++
Sbjct: 113 NSKIFLDLD 121
>NP_149037
          Length = 301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NSKSFVDI 8
           N K FVDI
Sbjct: 249 NIKGFVDI 256
>NP_001182163
          Length = 452

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SFVDIN 9
           SFVD+N
Sbjct: 421 SFVDVN 426
>NP_001098992
          Length = 452

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SFVDIN 9
           SFVD+N
Sbjct: 421 SFVDVN 426
>NP_001193556
          Length = 452

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SFVDIN 9
           SFVD+N
Sbjct: 421 SFVDVN 426
>NP_065091
          Length = 452

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SFVDIN 9
           SFVD+N
Sbjct: 421 SFVDVN 426
>NP_001193555
          Length = 452

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SFVDIN 9
           SFVD+N
Sbjct: 421 SFVDVN 426
>NP_001171700
          Length = 301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NSKSFVDI 8
           N K FVDI
Sbjct: 249 NIKGFVDI 256
>XP_006710017
          Length = 375

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SFVDIN 9
           SFVD+N
Sbjct: 344 SFVDVN 349
>XP_006710016
          Length = 452

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SFVDIN 9
           SFVD+N
Sbjct: 421 SFVDVN 426
>XP_005275771
          Length = 375

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SFVDIN 9
           SFVD+N
Sbjct: 344 SFVDVN 349
>XP_005275770
          Length = 452

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SFVDIN 9
           SFVD+N
Sbjct: 421 SFVDVN 426
>NP_001053
          Length = 433

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           K+F+DIN
Sbjct: 297 KTFLDIN 303
>NP_003115
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SKSFVDIN 9
           SK FVD++
Sbjct: 108 SKGFVDLS 115
>NP_110388
          Length = 351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SKSFVDI 8
           S+SFVD+
Sbjct: 166 SQSFVDV 172
>NP_003030
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   SKSFVDIN 9
           +K+F++IN
Sbjct: 465 AKTFIEIN 472
>NP_001578
          Length = 893

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KSFVDIN 9
           K+FVD N
Sbjct: 461 KAFVDFN 467
>NP_000267
          Length = 901

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KSFVDIN 9
           K+FVD N
Sbjct: 461 KAFVDFN 467
>NP_005497
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           ++FVDIN
Sbjct: 364 ETFVDIN 370
>NP_001191184
          Length = 335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           ++FVDIN
Sbjct: 221 ETFVDIN 227
>NP_997303
          Length = 512

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           K+FV+IN
Sbjct: 472 KTFVEIN 478
>NP_005015
          Length = 397

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFVDIN 9
           K+FV+IN
Sbjct: 339 KAFVEIN 345
>XP_005262479
          Length = 744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KSFVDIN 9
           K+FVD N
Sbjct: 304 KAFVDFN 310
>XP_005262478
          Length = 835

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KSFVDIN 9
           K+FVD N
Sbjct: 395 KAFVDFN 401
>XP_006724824
          Length = 890

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KSFVDIN 9
           K+FVD N
Sbjct: 458 KAFVDFN 464
>XP_006724823
          Length = 898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KSFVDIN 9
           K+FVD N
Sbjct: 458 KAFVDFN 464
>XP_006710899
          Length = 491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   SKSFVDIN 9
           +K+F++IN
Sbjct: 455 AKTFIEIN 462
>XP_005263548
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   SKSFVDIN 9
           +K+F++IN
Sbjct: 465 AKTFIEIN 472
>XP_005245954
          Length = 296

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SKSFVDI 8
           S+SFVD+
Sbjct: 111 SQSFVDV 117
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.125    0.325 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 430,934
Number of extensions: 889
Number of successful extensions: 58
Number of sequences better than 10000.0: 58
Number of HSP's gapped: 58
Number of HSP's successfully gapped: 58
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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