Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002515                                                              20     390
NP_057215                                                              17    4312
NP_001438                                                              17    4312
XP_006714824                                                           17    4312
NP_001248315                                                           16    5631
NP_001248311                                                           16    5631
NP_001248313                                                           16    5631
NP_001035787                                                           16    5631
NP_060784                                                              16    5631
NP_596870                                                              16    5631
NP_001248312                                                           16    5631
NP_001248314                                                           16    5631
XP_005253058                                                           16    5631
NP_689706                                                              16    7355
XP_005270565                                                           16    7355
XP_005270564                                                           16    7355
NP_689914                                                              15    9605
NP_001020029                                                           15    9605
NP_055637                                                              15    9605
NP_000338                                                              15    9605
XP_005258567                                                           15    9605
XP_006720304                                                           15    9605
XP_005268080                                                           15    9605
XP_005263436                                                           15    9605
XP_005263435                                                           15    9605
NP_055679                                                              15    9605
NP_789780                                                              15    9605
NP_940900                                                              15    9605
>NP_002515
          Length = 189

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  NSKSFANIN 9
          NSKSFA+IN
Sbjct: 86 NSKSFADIN 94
>NP_057215
          Length = 200

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSKSFANIN 9
           N KSF NI+
Sbjct: 93  NGKSFENIS 101
>NP_001438
          Length = 4349

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    NSKSFANIN 9
            NS+ F NIN
Sbjct: 1598 NSEGFFNIN 1606
>XP_006714824
          Length = 4349

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    NSKSFANIN 9
            NS+ F NIN
Sbjct: 1598 NSEGFFNIN 1606
>NP_001248315
          Length = 199

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  NSKSFAN 7
          NSKS+AN
Sbjct: 70 NSKSWAN 76
>NP_001248311
          Length = 449

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NSKSFAN 7
           NSKS+AN
Sbjct: 148 NSKSWAN 154
>NP_001248313
          Length = 341

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  NSKSFAN 7
          NSKS+AN
Sbjct: 40 NSKSWAN 46
>NP_001035787
          Length = 471

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NSKSFAN 7
           NSKS+AN
Sbjct: 170 NSKSWAN 176
>NP_060784
          Length = 379

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NSKSFAN 7
           NSKS+AN
Sbjct: 170 NSKSWAN 176
>NP_596870
          Length = 5604

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SKSFANIN 9
            +KSFAN++
Sbjct: 5264 AKSFANVD 5271
>NP_001248312
          Length = 357

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NSKSFAN 7
           NSKS+AN
Sbjct: 148 NSKSWAN 154
>NP_001248314
          Length = 341

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NSKSFAN 7
           NSKS+AN
Sbjct: 132 NSKSWAN 138
>XP_005253058
          Length = 215

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NSKSFAN 7
           NSKS+AN
Sbjct: 170 NSKSWAN 176
>NP_689706
          Length = 420

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SKSFAN 7
           SKSFAN
Sbjct: 261 SKSFAN 266
>XP_005270565
          Length = 407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SKSFAN 7
           SKSFAN
Sbjct: 248 SKSFAN 253
>XP_005270564
          Length = 407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SKSFAN 7
           SKSFAN
Sbjct: 248 SKSFAN 253
>NP_689914
          Length = 5058

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NSKSFANI 8
            N+K+F NI
Sbjct: 5008 NNKTFLNI 5015
>NP_001020029
          Length = 2328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NSKSFAN 7
           NS+ FAN
Sbjct: 326 NSRKFAN 332
>NP_055637
          Length = 1032

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSKSFANIN 9
           N  SFA+IN
Sbjct: 945 NVPSFAHIN 953
>NP_000338
          Length = 2137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NSKSFAN 7
           NS+ FAN
Sbjct: 326 NSRKFAN 332
>XP_005258567
          Length = 1036

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    NSKSFANIN 9
            +SKS  N+N
Sbjct: 1006 SSKSLVNLN 1014
>XP_006720304
          Length = 2328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NSKSFAN 7
           NS+ FAN
Sbjct: 326 NSRKFAN 332
>XP_005268080
          Length = 2328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NSKSFAN 7
           NS+ FAN
Sbjct: 326 NSRKFAN 332
>XP_005263436
          Length = 1033

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSKSFANIN 9
           N  SFA+IN
Sbjct: 946 NVPSFAHIN 954
>XP_005263435
          Length = 1033

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSKSFANIN 9
           N  SFA+IN
Sbjct: 946 NVPSFAHIN 954
>NP_055679
          Length = 409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NSKSFAN 7
           N+KSF N
Sbjct: 310 NAKSFGN 316
>NP_789780
          Length = 1043

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    NSKSFANIN 9
            +SKS  N+N
Sbjct: 1006 SSKSLVNLN 1014
>NP_940900
          Length = 236

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  NSKSFAN 7
          NSK F+N
Sbjct: 25 NSKQFSN 31
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.301    0.113    0.286 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 385,076
Number of extensions: 548
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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