Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001243398                                                           20     390
NP_036462                                                              20     390
NP_001243397                                                           20     390
XP_005269721                                                           20     390
XP_005248564                                                           20     509
NP_005900                                                              20     509
NP_001191055                                                           17    3301
NP_006143                                                              17    3301
NP_001191056                                                           17    3301
XP_005253431                                                           17    3301
XP_006719140                                                           17    3301
XP_006719139                                                           17    3301
XP_005253429                                                           17    3301
XP_006719138                                                           17    3301
XP_005253428                                                           17    3301
XP_005253427                                                           17    3301
NP_009057                                                              17    4312
NP_689916                                                              17    4312
XP_006715953                                                           17    4312
XP_006715952                                                           17    4312
XP_005250250                                                           17    4312
XP_006715951                                                           17    4312
XP_005250248                                                           17    4312
NP_777549                                                              16    5631
NP_001269470                                                           16    5631
NP_003167                                                              16    5631
NP_001269471                                                           16    5631
XP_005271213                                                           16    5631
XP_005271212                                                           16    5631
XP_005271211                                                           16    5631
XP_006710922                                                           16    5631
NP_000375                                                              16    7355
NP_775321                                                              16    7355
NP_001106649                                                           16    7355
NP_619728                                                              16    7355
NP_006590                                                              16    7355
NP_619727                                                              16    7355
NP_775322                                                              16    7355
NP_149022                                                              16    7355
NP_001072                                                              16    7355
NP_001004316                                                           16    7355
NP_004432                                                              16    7355
XP_005255834                                                           16    7355
XP_006721188                                                           16    7355
XP_006713699                                                           16    7355
XP_006713697                                                           16    7355
XP_006713696                                                           16    7355
XP_005247498                                                           16    7355
XP_006713694                                                           16    7355
NP_940927                                                              15    9605
NP_004353                                                              15    9605
NP_001124147                                                           15    9605
NP_006436                                                              15    9605
NP_056023                                                              15    9605
NP_071922                                                              15    9605
NP_001190985                                                           15    9605
NP_001077094                                                           15    9605
NP_003874                                                              15    9605
NP_001124320                                                           15    9605
NP_001269964                                                           15    9605
NP_004901                                                              15    9605
XP_006726620                                                           15    9605
XP_006722380                                                           15    9605
XP_005258153                                                           15    9605
XP_005258152                                                           15    9605
XP_005258151                                                           15    9605
XP_005258150                                                           15    9605
XP_005258149                                                           15    9605
XP_006720857                                                           15    9605
XP_006720560                                                           15    9605
XP_005254961                                                           15    9605
XP_005254960                                                           15    9605
XP_005254959                                                           15    9605
XP_006720422                                                           15    9605
XP_006714125                                                           15    9605
>NP_001243398
          Length = 1781

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NSKEVDIEF 9
            NSKEVD EF
Sbjct: 1227 NSKEVDTEF 1235
>NP_036462
          Length = 2073

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NSKEVDIEF 9
            NSKEVD EF
Sbjct: 1519 NSKEVDTEF 1527
>NP_001243397
          Length = 1890

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NSKEVDIEF 9
            NSKEVD EF
Sbjct: 1336 NSKEVDTEF 1344
>XP_005269721
          Length = 2073

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NSKEVDIEF 9
            NSKEVD EF
Sbjct: 1519 NSKEVDTEF 1527
>XP_005248564
          Length = 2342

 Score = 19.6 bits (39), Expect =   509
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    NSKEVDIEF 9
            NSK VD+EF
Sbjct: 2242 NSKNVDVEF 2250
>NP_005900
          Length = 2468

 Score = 19.6 bits (39), Expect =   509
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    NSKEVDIEF 9
            NSK VD+EF
Sbjct: 2368 NSKNVDVEF 2376
>NP_001191055
          Length = 499

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSKEVDIEF 9
           N KEV+ EF
Sbjct: 150 NEKEVEAEF 158
>NP_006143
          Length = 499

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSKEVDIEF 9
           N KEV+ EF
Sbjct: 150 NEKEVEAEF 158
>NP_001191056
          Length = 499

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSKEVDIEF 9
           N KEV+ EF
Sbjct: 150 NEKEVEAEF 158
>XP_005253431
          Length = 499

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSKEVDIEF 9
           N KEV+ EF
Sbjct: 150 NEKEVEAEF 158
>XP_006719140
          Length = 446

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSKEVDIEF 9
           N KEV+ EF
Sbjct: 97  NEKEVEAEF 105
>XP_006719139
          Length = 499

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSKEVDIEF 9
           N KEV+ EF
Sbjct: 150 NEKEVEAEF 158
>XP_005253429
          Length = 499

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSKEVDIEF 9
           N KEV+ EF
Sbjct: 150 NEKEVEAEF 158
>XP_006719138
          Length = 499

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSKEVDIEF 9
           N KEV+ EF
Sbjct: 150 NEKEVEAEF 158
>XP_005253428
          Length = 499

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSKEVDIEF 9
           N KEV+ EF
Sbjct: 150 NEKEVEAEF 158
>XP_005253427
          Length = 499

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSKEVDIEF 9
           N KEV+ EF
Sbjct: 150 NEKEVEAEF 158
>NP_009057
          Length = 806

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKEVDIEF 9
           +K+VD+EF
Sbjct: 667 AKDVDLEF 674
>NP_689916
          Length = 1584

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NSKEVDIEF 9
           NSK+ D++F
Sbjct: 355 NSKQRDVDF 363
>XP_006715953
          Length = 1584

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NSKEVDIEF 9
           NSK+ D++F
Sbjct: 355 NSKQRDVDF 363
>XP_006715952
          Length = 1584

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NSKEVDIEF 9
           NSK+ D++F
Sbjct: 355 NSKQRDVDF 363
>XP_005250250
          Length = 1584

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NSKEVDIEF 9
           NSK+ D++F
Sbjct: 355 NSKQRDVDF 363
>XP_006715951
          Length = 1584

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NSKEVDIEF 9
           NSK+ D++F
Sbjct: 355 NSKQRDVDF 363
>XP_005250248
          Length = 1584

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NSKEVDIEF 9
           NSK+ D++F
Sbjct: 355 NSKQRDVDF 363
>NP_777549
          Length = 169

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  NSKEVDIE 8
          N KEVD E
Sbjct: 56 NKKEVDYE 63
>NP_001269470
          Length = 976

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSKEVDIE 8
           N+KEV++E
Sbjct: 427 NNKEVELE 434
>NP_003167
          Length = 976

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSKEVDIE 8
           N+KEV++E
Sbjct: 427 NNKEVELE 434
>NP_001269471
          Length = 951

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSKEVDIE 8
           N+KEV++E
Sbjct: 427 NNKEVELE 434
>XP_005271213
          Length = 921

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSKEVDIE 8
           N+KEV++E
Sbjct: 372 NNKEVELE 379
>XP_005271212
          Length = 958

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSKEVDIE 8
           N+KEV++E
Sbjct: 409 NNKEVELE 416
>XP_005271211
          Length = 973

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSKEVDIE 8
           N+KEV++E
Sbjct: 424 NNKEVELE 431
>XP_006710922
          Length = 976

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSKEVDIE 8
           N+KEV++E
Sbjct: 427 NNKEVELE 434
>NP_000375
          Length = 4563

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSKEVDIE 8
           NS+E+DI+
Sbjct: 602 NSEELDIQ 609
>NP_775321
          Length = 1455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KEVDIE
Sbjct: 282 KEVDIE 287
>NP_001106649
          Length = 1548

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KEVDIE
Sbjct: 376 KEVDIE 381
>NP_619728
          Length = 1455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KEVDIE
Sbjct: 282 KEVDIE 287
>NP_006590
          Length = 1531

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KEVDIE
Sbjct: 358 KEVDIE 363
>NP_619727
          Length = 1549

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KEVDIE
Sbjct: 376 KEVDIE 381
>NP_775322
          Length = 1455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KEVDIE
Sbjct: 282 KEVDIE 287
>NP_149022
          Length = 513

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NSKEVDIE 8
           NS+E+D++
Sbjct: 279 NSRELDVD 286
>NP_001072
          Length = 3623

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NSKEVDIE 8
           NSK VD+E
Sbjct: 114 NSKLVDLE 121
>NP_001004316
          Length = 692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NSKEVDIE 8
           NSKE +IE
Sbjct: 491 NSKEKEIE 498
>NP_004432
          Length = 984

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SKEVDIEF 9
           +KE+D+ F
Sbjct: 611 AKEIDVSF 618
>XP_005255834
          Length = 1264

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  KEVDIE 8
          KEVDIE
Sbjct: 91 KEVDIE 96
>XP_006721188
          Length = 1484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KEVDIE
Sbjct: 311 KEVDIE 316
>XP_006713699
          Length = 425

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NSKEVDIE 8
           NSKE +IE
Sbjct: 224 NSKEKEIE 231
>XP_006713697
          Length = 599

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NSKEVDIE 8
           NSKE +IE
Sbjct: 398 NSKEKEIE 405
>XP_006713696
          Length = 613

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NSKEVDIE 8
           NSKE +IE
Sbjct: 412 NSKEKEIE 419
>XP_005247498
          Length = 692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NSKEVDIE 8
           NSKE +IE
Sbjct: 491 NSKEKEIE 498
>XP_006713694
          Length = 859

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NSKEVDIE 8
           NSKE +IE
Sbjct: 658 NSKEKEIE 665
>NP_940927
          Length = 1343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KEVD+E
Sbjct: 182 KEVDVE 187
>NP_004353
          Length = 610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  NSKEVDI 7
          NS+E+D+
Sbjct: 34 NSEEIDV 40
>NP_001124147
          Length = 610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  NSKEVDI 7
          NS+E+D+
Sbjct: 34 NSEEIDV 40
>NP_006436
          Length = 2335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   KEVDIEF 9
           KEV IEF
Sbjct: 892 KEVGIEF 898
>NP_056023
          Length = 2062

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KE+DIE
Sbjct: 804 KEIDIE 809
>NP_071922
          Length = 363

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  NSKEVDIE 8
          N KE DIE
Sbjct: 17 NDKEHDIE 24
>NP_001190985
          Length = 2039

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KE+DIE
Sbjct: 781 KEIDIE 786
>NP_001077094
          Length = 2039

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KE+DIE
Sbjct: 781 KEIDIE 786
>NP_003874
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NSKEVDIE 8
           N KE D+E
Sbjct: 420 NDKESDVE 427
>NP_001124320
          Length = 1243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NSKEVDIE 8
           NSK+ D++
Sbjct: 447 NSKQADVQ 454
>NP_001269964
          Length = 336

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  NSKEVDIEF 9
          N KE D+E+
Sbjct: 44 NGKEWDMEY 52
>NP_004901
          Length = 1244

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NSKEVDIE 8
           NSK+ D++
Sbjct: 447 NSKQADVQ 454
>XP_006726620
          Length = 106

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SKEVDIEF 9
          S +VD+EF
Sbjct: 33 SHQVDLEF 40
>XP_006722380
          Length = 1769

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KE+DIE
Sbjct: 511 KEIDIE 516
>XP_005258153
          Length = 2037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KE+DIE
Sbjct: 779 KEIDIE 784
>XP_005258152
          Length = 2060

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KE+DIE
Sbjct: 802 KEIDIE 807
>XP_005258151
          Length = 2062

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KE+DIE
Sbjct: 804 KEIDIE 809
>XP_005258150
          Length = 2062

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KE+DIE
Sbjct: 804 KEIDIE 809
>XP_005258149
          Length = 2062

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KE+DIE
Sbjct: 804 KEIDIE 809
>XP_006720857
          Length = 106

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SKEVDIEF 9
          S +VD+EF
Sbjct: 33 SHQVDLEF 40
>XP_006720560
          Length = 1313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KEVD+E
Sbjct: 182 KEVDVE 187
>XP_005254961
          Length = 1342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KEVD+E
Sbjct: 182 KEVDVE 187
>XP_005254960
          Length = 1342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KEVD+E
Sbjct: 182 KEVDVE 187
>XP_005254959
          Length = 1343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVDIE 8
           KEVD+E
Sbjct: 182 KEVDVE 187
>XP_006720422
          Length = 106

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SKEVDIEF 9
          S +VD+EF
Sbjct: 33 SHQVDLEF 40
>XP_006714125
          Length = 610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  NSKEVDI 7
          NS+E+D+
Sbjct: 34 NSEEIDV 40
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.306    0.130    0.327 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 558,250
Number of extensions: 1108
Number of successful extensions: 75
Number of sequences better than 10000.0: 75
Number of HSP's gapped: 75
Number of HSP's successfully gapped: 75
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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