Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001167558                                                           20     509
NP_858045                                                              20     509
NP_006525                                                              20     509
NP_001167559                                                           20     509
NP_000048                                                              18    1482
NP_001274177                                                           18    1482
NP_001274176                                                           18    1482
NP_001274175                                                           18    1482
XP_006720695                                                           18    1482
NP_110379                                                              17    3301
NP_001008897                                                           17    3301
NP_996816                                                              16    5631
NP_003150                                                              16    7355
NP_001032420                                                           16    7355
NP_060177                                                              16    7355
XP_005274642                                                           16    7355
XP_005274641                                                           16    7355
XP_006724574                                                           16    7355
XP_005245991                                                           16    7355
NP_001137250                                                           16    7355
NP_000089                                                              15    9605
NP_001094383                                                           15    9605
NP_056012                                                              15    9605
NP_001269889                                                           15    9605
NP_001269888                                                           15    9605
NP_060707                                                              15    9605
NP_004570                                                              15    9605
NP_001230056                                                           15    9605
NP_055532                                                              15    9605
NP_055501                                                              15    9605
NP_008988                                                              15    9605
NP_001005413                                                           15    9605
NP_127490                                                              15    9605
XP_006723575                                                           15    9605
XP_005259502                                                           15    9605
XP_005259501                                                           15    9605
XP_006720155                                                           15    9605
XP_006720154                                                           15    9605
XP_006720153                                                           15    9605
XP_006720152                                                           15    9605
XP_006720151                                                           15    9605
XP_006720150                                                           15    9605
XP_005267520                                                           15    9605
XP_006720149                                                           15    9605
XP_005267519                                                           15    9605
XP_006720148                                                           15    9605
XP_006718714                                                           15    9605
XP_005274200                                                           15    9605
XP_006718713                                                           15    9605
XP_005274199                                                           15    9605
XP_005269520                                                           15    9605
XP_005270541                                                           15    9605
>NP_001167558
          Length = 1423

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSKAGIMEP 9
           NSKAG MEP
Sbjct: 924 NSKAGRMEP 932
>NP_858045
          Length = 1424

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSKAGIMEP 9
           NSKAG MEP
Sbjct: 924 NSKAGRMEP 932
>NP_006525
          Length = 1420

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSKAGIMEP 9
           NSKAG MEP
Sbjct: 924 NSKAGRMEP 932
>NP_001167559
          Length = 1415

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSKAGIMEP 9
           NSKAG MEP
Sbjct: 919 NSKAGRMEP 927
>NP_000048
          Length = 1417

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    NSKAGIMEP 9
            NSK GIM P
Sbjct: 1393 NSKLGIMAP 1401
>NP_001274177
          Length = 1042

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    NSKAGIMEP 9
            NSK GIM P
Sbjct: 1018 NSKLGIMAP 1026
>NP_001274176
          Length = 1286

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    NSKAGIMEP 9
            NSK GIM P
Sbjct: 1262 NSKLGIMAP 1270
>NP_001274175
          Length = 1417

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    NSKAGIMEP 9
            NSK GIM P
Sbjct: 1393 NSKLGIMAP 1401
>XP_006720695
          Length = 763

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   NSKAGIMEP 9
           NSK GIM P
Sbjct: 739 NSKLGIMAP 747
>NP_110379
          Length = 556

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NSKAGIMEP 9
           N +AG+ EP
Sbjct: 498 NKQAGVFEP 506
>NP_001008897
          Length = 401

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NSKAGIMEP 9
           N +AG+ EP
Sbjct: 343 NKQAGVFEP 351
>NP_996816
          Length = 5202

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    NSKAGIMEP 9
            NS+ GI+ P
Sbjct: 4513 NSQGGILSP 4521
>NP_003150
          Length = 1030

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SKAGIMEP 9
           SKAG ++P
Sbjct: 453 SKAGTLQP 460
>NP_001032420
          Length = 1030

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SKAGIMEP 9
           SKAG ++P
Sbjct: 453 SKAGTLQP 460
>NP_060177
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NSKAGIMEP 9
           NS+ G+ EP
Sbjct: 817 NSEEGLREP 825
>XP_005274642
          Length = 984

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SKAGIMEP 9
           SKAG ++P
Sbjct: 436 SKAGTLQP 443
>XP_005274641
          Length = 1001

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SKAGIMEP 9
           SKAG ++P
Sbjct: 453 SKAGTLQP 460
>XP_006724574
          Length = 1030

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SKAGIMEP 9
           SKAG ++P
Sbjct: 453 SKAGTLQP 460
>XP_005245991
          Length = 905

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NSKAGIMEP 9
           NS+ G+ EP
Sbjct: 819 NSEEGLREP 827
>NP_001137250
          Length = 894

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NSKAGIMEP 9
           NS+ G+ EP
Sbjct: 808 NSEEGLREP 816
>NP_000089
          Length = 658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KAGIMEP 9
           +AG++EP
Sbjct: 167 RAGLLEP 173
>NP_001094383
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           K GI+EP
Sbjct: 695 KVGIVEP 701
>NP_056012
          Length = 889

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           K GI+EP
Sbjct: 680 KVGIVEP 686
>NP_001269889
          Length = 1135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KAGIMEP 9
           KAG+++P
Sbjct: 260 KAGLLQP 266
>NP_001269888
          Length = 1135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KAGIMEP 9
           KAG+++P
Sbjct: 260 KAGLLQP 266
>NP_060707
          Length = 1150

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KAGIMEP 9
           KAG+++P
Sbjct: 275 KAGLLQP 281
>NP_004570
          Length = 820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SKAGIMEP 9
           S AG++EP
Sbjct: 646 SPAGMLEP 653
>NP_001230056
          Length = 814

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           K GI+EP
Sbjct: 605 KVGIVEP 611
>NP_055532
          Length = 1300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           KAG+ EP
Sbjct: 443 KAGLSEP 449
>NP_055501
          Length = 1070

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           KAG M+P
Sbjct: 683 KAGAMQP 689
>NP_008988
          Length = 277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  AGIMEP 9
          AGI+EP
Sbjct: 20 AGILEP 25
>NP_001005413
          Length = 230

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  AGIMEP 9
          AGI+EP
Sbjct: 20 AGILEP 25
>NP_127490
          Length = 277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  AGIMEP 9
          AGI+EP
Sbjct: 20 AGILEP 25
>XP_006723575
          Length = 1302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           KAG+ EP
Sbjct: 443 KAGLSEP 449
>XP_005259502
          Length = 1313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           KAG+ EP
Sbjct: 456 KAGLSEP 462
>XP_005259501
          Length = 1070

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           KAG M+P
Sbjct: 683 KAGAMQP 689
>XP_006720155
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           K GI+EP
Sbjct: 672 KVGIVEP 678
>XP_006720154
          Length = 894

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           K GI+EP
Sbjct: 685 KVGIVEP 691
>XP_006720153
          Length = 905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           K GI+EP
Sbjct: 696 KVGIVEP 702
>XP_006720152
          Length = 911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           K GI+EP
Sbjct: 681 KVGIVEP 687
>XP_006720151
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           K GI+EP
Sbjct: 685 KVGIVEP 691
>XP_006720150
          Length = 918

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           K GI+EP
Sbjct: 688 KVGIVEP 694
>XP_005267520
          Length = 921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           K GI+EP
Sbjct: 691 KVGIVEP 697
>XP_006720149
          Length = 922

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           K GI+EP
Sbjct: 692 KVGIVEP 698
>XP_005267519
          Length = 925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           K GI+EP
Sbjct: 695 KVGIVEP 701
>XP_006720148
          Length = 926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   KAGIMEP 9
           K GI+EP
Sbjct: 696 KVGIVEP 702
>XP_006718714
          Length = 637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SKAGIMEP 9
           S AG++EP
Sbjct: 463 SPAGMLEP 470
>XP_005274200
          Length = 816

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SKAGIMEP 9
           S AG++EP
Sbjct: 642 SPAGMLEP 649
>XP_006718713
          Length = 837

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SKAGIMEP 9
           S AG++EP
Sbjct: 686 SPAGMLEP 693
>XP_005274199
          Length = 860

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SKAGIMEP 9
           S AG++EP
Sbjct: 686 SPAGMLEP 693
>XP_005269520
          Length = 277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  AGIMEP 9
          AGI+EP
Sbjct: 20 AGILEP 25
>XP_005270541
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KAGIMEP 9
           +AG++EP
Sbjct: 167 RAGLLEP 173
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.301    0.122    0.331 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 482,578
Number of extensions: 843
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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