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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              18    1135
NP_071351                                                              17    3301
NP_001124178                                                           17    3301
NP_001269899                                                           17    3301
NP_001124180                                                           17    3301
NP_068763                                                              17    3301
NP_001269897                                                           17    3301
NP_001269898                                                           17    3301
XP_006720295                                                           17    3301
XP_006720294                                                           17    3301
XP_005268030                                                           17    3301
XP_005268029                                                           17    3301
XP_005268028                                                           17    3301
XP_006720293                                                           17    3301
XP_006720292                                                           17    3301
NP_001093112                                                           16    5631
NP_060818                                                              16    5631
XP_005256104                                                           16    5631
XP_005256103                                                           16    5631
NP_005250                                                              15    9605
NP_056161                                                              15    9605
NP_061992                                                              15    9605
NP_689646                                                              15    9605
NP_940841                                                              15    9605
NP_055495                                                              15    9605
XP_006722261                                                           15    9605
XP_005258130                                                           15    9605
XP_006720156                                                           15    9605
XP_006718830                                                           15    9605
XP_006712433                                                           15    9605
XP_006711947                                                           15    9605
XP_006711945                                                           15    9605
NP_055422                                                              15    9605

>NP_115803
          Length = 1156

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFLDIKII 9
           NSFLDIK I
Sbjct: 198 NSFLDIKRI 206



>NP_071351
          Length = 2752

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NSFLDIKII 9
            N+F+DI II
Sbjct: 2609 NNFMDITII 2617



>NP_001124178
          Length = 538

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSFLDIKI 8
           NSF++IK+
Sbjct: 527 NSFIEIKV 534



>NP_001269899
          Length = 498

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSFLDIKI 8
           NSF++IK+
Sbjct: 487 NSFIEIKV 494



>NP_001124180
          Length = 538

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSFLDIKI 8
           NSF++IK+
Sbjct: 527 NSFIEIKV 534



>NP_068763
          Length = 538

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSFLDIKI 8
           NSF++IK+
Sbjct: 527 NSFIEIKV 534



>NP_001269897
          Length = 358

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSFLDIKI 8
           NSF++IK+
Sbjct: 347 NSFIEIKV 354



>NP_001269898
          Length = 358

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSFLDIKI 8
           NSF++IK+
Sbjct: 347 NSFIEIKV 354



>XP_006720295
          Length = 358

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSFLDIKI 8
           NSF++IK+
Sbjct: 347 NSFIEIKV 354



>XP_006720294
          Length = 358

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSFLDIKI 8
           NSF++IK+
Sbjct: 347 NSFIEIKV 354



>XP_005268030
          Length = 538

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSFLDIKI 8
           NSF++IK+
Sbjct: 527 NSFIEIKV 534



>XP_005268029
          Length = 538

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSFLDIKI 8
           NSF++IK+
Sbjct: 527 NSFIEIKV 534



>XP_005268028
          Length = 538

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSFLDIKI 8
           NSF++IK+
Sbjct: 527 NSFIEIKV 534



>XP_006720293
          Length = 538

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSFLDIKI 8
           NSF++IK+
Sbjct: 527 NSFIEIKV 534



>XP_006720292
          Length = 538

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NSFLDIKI 8
           NSF++IK+
Sbjct: 527 NSFIEIKV 534



>NP_001093112
          Length = 770

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  NSFLDIK 7
          N+FLD+K
Sbjct: 61 NAFLDLK 67



>NP_060818
          Length = 770

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  NSFLDIK 7
          N+FLD+K
Sbjct: 61 NAFLDLK 67



>XP_005256104
          Length = 770

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  NSFLDIK 7
          N+FLD+K
Sbjct: 61 NAFLDLK 67



>XP_005256103
          Length = 770

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  NSFLDIK 7
          N+FLD+K
Sbjct: 61 NAFLDLK 67



>NP_005250
          Length = 375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NSFLDIKI 8
           N FL++K+
Sbjct: 250 NPFLEVKV 257



>NP_056161
          Length = 2539

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NSFLDIK 7
           +SFLD+K
Sbjct: 663 HSFLDLK 669



>NP_061992
          Length = 797

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NSFLDIKI 8
           NSF  IKI
Sbjct: 175 NSFFHIKI 182



>NP_689646
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  NSFLDIKII 9
          N F D KII
Sbjct: 49 NVFYDFKII 57



>NP_940841
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  NSFLDIKII 9
          N F D KII
Sbjct: 49 NVFYDFKII 57



>NP_055495
          Length = 2235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NSFLDIKI 8
            NSF+ IKI
Sbjct: 2113 NSFIYIKI 2120



>XP_006722261
          Length = 2092

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NSFLDIKI 8
            NSF+ IKI
Sbjct: 1970 NSFIYIKI 1977



>XP_005258130
          Length = 2234

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    NSFLDIKI 8
            NSF+ IKI
Sbjct: 2112 NSFIYIKI 2119



>XP_006720156
          Length = 2497

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NSFLDIK 7
           +SFLD+K
Sbjct: 663 HSFLDLK 669



>XP_006718830
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  NSFLDIKII 9
          N F D KII
Sbjct: 49 NVFYDFKII 57



>XP_006712433
          Length = 322

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NSFLDIKI 8
          NSF+ IKI
Sbjct: 51 NSFVAIKI 58



>XP_006711947
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NSFLDIKI 8
           N+F D+K+
Sbjct: 262 NAFSDVKV 269



>XP_006711945
          Length = 630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NSFLDIKI 8
           N+F D+K+
Sbjct: 451 NAFSDVKV 458



>NP_055422
          Length = 508

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NSFLDIKI 8
          NSF+ IKI
Sbjct: 51 NSFVAIKI 58



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.155    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 465,324
Number of extensions: 617
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)