Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1135
NP_001263689                                                           18    1135
NP_001119584                                                           18    1135
NP_001119586                                                           18    1135
NP_001263625                                                           18    1135
NP_001119590                                                           18    1135
NP_001263690                                                           18    1135
NP_001119585                                                           18    1135
NP_001263624                                                           18    1135
NP_001119588                                                           18    1135
NP_001119587                                                           18    1135
NP_001119589                                                           18    1135
NP_001263627                                                           18    1135
NP_001263628                                                           18    1135
NP_001263626                                                           18    1135
NP_996555                                                              17    4312
NP_001241649                                                           17    4312
NP_001241647                                                           17    4312
NP_001241650                                                           17    4312
NP_001241652                                                           17    4312
NP_002456                                                              17    4312
NP_001241651                                                           17    4312
NP_996557                                                              17    4312
NP_001241648                                                           17    4312
NP_996556                                                              17    4312
NP_542415                                                              17    4312
NP_542416                                                              17    4312
NP_542414                                                              17    4312
NP_006255                                                              17    4312
XP_006719474                                                           17    4312
XP_005268933                                                           17    4312
XP_006719473                                                           17    4312
XP_006719472                                                           17    4312
XP_006719471                                                           17    4312
XP_006719470                                                           17    4312
XP_006719469                                                           17    4312
XP_006719468                                                           17    4312
XP_005263224                                                           17    4312
NP_001269889                                                           16    5631
NP_001252542                                                           16    5631
NP_001269888                                                           16    5631
NP_060707                                                              16    5631
NP_001010985                                                           16    5631
NP_006368                                                              16    5631
XP_006716779                                                           16    5631
XP_006710676                                                           16    5631
NP_001157032                                                           16    7355
NP_689977                                                              16    7355
NP_065948                                                              15    9605
NP_004988                                                              15    9605
NP_064551                                                              15    9605
NP_004524                                                              15    9605
XP_005276805                                                           15    9605
XP_006713765                                                           15    9605
XP_005245431                                                           15    9605
XP_005245254                                                           15    9605
XP_005245253                                                           15    9605
>NP_000537
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           NSFEVRV A
Sbjct: 268 NSFEVRVCA 276
>NP_001263689
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           NSFEVRV A
Sbjct: 229 NSFEVRVCA 237
>NP_001119584
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           NSFEVRV A
Sbjct: 268 NSFEVRVCA 276
>NP_001119586
          Length = 341

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           NSFEVRV A
Sbjct: 268 NSFEVRVCA 276
>NP_001263625
          Length = 302

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           NSFEVRV A
Sbjct: 229 NSFEVRVCA 237
>NP_001119590
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           NSFEVRV A
Sbjct: 229 NSFEVRVCA 237
>NP_001263690
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           NSFEVRV A
Sbjct: 229 NSFEVRVCA 237
>NP_001119585
          Length = 346

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           NSFEVRV A
Sbjct: 268 NSFEVRVCA 276
>NP_001263624
          Length = 307

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           NSFEVRV A
Sbjct: 229 NSFEVRVCA 237
>NP_001119588
          Length = 209

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           NSFEVRV A
Sbjct: 136 NSFEVRVCA 144
>NP_001119587
          Length = 261

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           NSFEVRV A
Sbjct: 136 NSFEVRVCA 144
>NP_001119589
          Length = 214

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           NSFEVRV A
Sbjct: 136 NSFEVRVCA 144
>NP_001263627
          Length = 182

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           NSFEVRV A
Sbjct: 109 NSFEVRVCA 117
>NP_001263628
          Length = 187

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           NSFEVRV A
Sbjct: 109 NSFEVRVCA 117
>NP_001263626
          Length = 234

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           NSFEVRV A
Sbjct: 109 NSFEVRVCA 117
>NP_996555
          Length = 1148

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 714 AYEVRIFA 721
>NP_001241649
          Length = 1134

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 677 AYEVRIFA 684
>NP_001241647
          Length = 1173

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 689 AYEVRIFA 696
>NP_001241650
          Length = 1127

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 670 AYEVRIFA 677
>NP_001241652
          Length = 1139

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 676 AYEVRIFA 683
>NP_002456
          Length = 1171

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 714 AYEVRIFA 721
>NP_001241651
          Length = 1120

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 663 AYEVRIFA 670
>NP_996557
          Length = 1123

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 689 AYEVRIFA 696
>NP_001241648
          Length = 1146

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 689 AYEVRIFA 696
>NP_996556
          Length = 1141

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 689 AYEVRIFA 696
>NP_542415
          Length = 2294

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    NSFEVRVF 8
            N+FEV++F
Sbjct: 1307 NTFEVKLF 1314
>NP_542416
          Length = 2490

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    NSFEVRVF 8
            N+FEV++F
Sbjct: 1503 NTFEVKLF 1510
>NP_542414
          Length = 2485

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    NSFEVRVF 8
            N+FEV++F
Sbjct: 1498 NTFEVKLF 1505
>NP_006255
          Length = 2466

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    NSFEVRVF 8
            N+FEV++F
Sbjct: 1479 NTFEVKLF 1486
>XP_006719474
          Length = 1164

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 689 AYEVRIFA 696
>XP_005268933
          Length = 1165

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 702 AYEVRIFA 709
>XP_006719473
          Length = 1166

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 714 AYEVRIFA 721
>XP_006719472
          Length = 1170

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 689 AYEVRIFA 696
>XP_006719471
          Length = 1177

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 714 AYEVRIFA 721
>XP_006719470
          Length = 1183

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 702 AYEVRIFA 709
>XP_006719469
          Length = 1189

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 714 AYEVRIFA 721
>XP_006719468
          Length = 1195

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SFEVRVFA 9
           ++EVR+FA
Sbjct: 714 AYEVRIFA 721
>XP_005263224
          Length = 2491

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    NSFEVRVF 8
            N+FEV++F
Sbjct: 1504 NTFEVKLF 1511
>NP_001269889
          Length = 1135

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           +SFEV +FA
Sbjct: 748 HSFEVSLFA 756
>NP_001252542
          Length = 331

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSFEVRVFA 9
           NS+  RVFA
Sbjct: 190 NSYAFRVFA 198
>NP_001269888
          Length = 1135

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           +SFEV +FA
Sbjct: 748 HSFEVSLFA 756
>NP_060707
          Length = 1150

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           +SFEV +FA
Sbjct: 763 HSFEVSLFA 771
>NP_001010985
          Length = 354

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSFEVRVFA 9
           NS+  RVFA
Sbjct: 213 NSYAFRVFA 221
>NP_006368
          Length = 1591

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    NSFEVRV 7
            NSF+VR+
Sbjct: 1224 NSFQVRI 1230
>XP_006716779
          Length = 2399

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    NSFEVRV 7
            NSF+VR+
Sbjct: 2032 NSFQVRI 2038
>XP_006710676
          Length = 354

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NSFEVRVFA 9
           NS+  RVFA
Sbjct: 213 NSYAFRVFA 221
>NP_001157032
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  NSFEVRVF 8
          NSF  R+F
Sbjct: 3  NSFAARIF 10
>NP_689977
          Length = 442

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  NSFEVRVF 8
          NSF  R+F
Sbjct: 3  NSFAARIF 10
>NP_065948
          Length = 774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  NSFEVRVF 8
          N F +RVF
Sbjct: 58 NKFSLRVF 65
>NP_004988
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NSFEVRVFA 9
           NS+  RVF+
Sbjct: 334 NSYSFRVFS 342
>NP_064551
          Length = 725

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           ++FE R++A
Sbjct: 380 HAFEARIYA 388
>NP_004524
          Length = 1141

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FEVRVFA 9
           +E+RVFA
Sbjct: 709 YEMRVFA 715
>XP_005276805
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  NSFEVRVF 8
          N F +RVF
Sbjct: 58 NKFSLRVF 65
>XP_006713765
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   NSFEVRVFA 9
           ++FE R++A
Sbjct: 271 HAFEARIYA 279
>XP_005245431
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  NSFEVRVF 8
          N F +RVF
Sbjct: 58 NKFSLRVF 65
>XP_005245254
          Length = 315

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NSFEVRVFA 9
           NS+  RVF+
Sbjct: 172 NSYSFRVFS 180
>XP_005245253
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NSFEVRVFA 9
           NS+  RVF+
Sbjct: 334 NSYSFRVFS 342
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.139    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 379,997
Number of extensions: 473
Number of successful extensions: 57
Number of sequences better than 10000.0: 57
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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