Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001135993                                                           18    1935
NP_001135991                                                           18    1935
NP_071357                                                              18    1935
NP_942144                                                              18    1935
NP_001135992                                                           18    1935
NP_004459                                                              17    3301
NP_001230807                                                           17    3301
XP_005270683                                                           17    3301
NP_002221                                                              16    5631
NP_000050                                                              16    5631
NP_068831                                                              16    5631
NP_001159906                                                           16    5631
NP_002206                                                              16    5631
NP_005240                                                              16    5631
NP_777576                                                              16    5631
XP_006721941                                                           16    5631
XP_006721940                                                           16    5631
XP_006721939                                                           16    5631
XP_006721938                                                           16    5631
XP_006721937                                                           16    5631
XP_006721936                                                           16    5631
XP_006721935                                                           16    5631
XP_006721934                                                           16    5631
XP_005257370                                                           16    5631
NP_001164409                                                           16    7355
NP_057452                                                              16    7355
NP_001240318                                                           16    7355
NP_001269472                                                           16    7355
NP_000456                                                              16    7355
XP_006722829                                                           16    7355
NP_699189                                                              16    7355
NP_001004731                                                           16    7355
NP_001154824                                                           15    9605
NP_059138                                                              15    9605
NP_060664                                                              15    9605
NP_001164059                                                           15    9605
NP_001116306                                                           15    9605
NP_001164035                                                           15    9605
NP_001164051                                                           15    9605
NP_055520                                                              15    9605
NP_149981                                                              15    9605
NP_001164058                                                           15    9605
NP_001139806                                                           15    9605
XP_006726609                                                           15    9605
XP_005260222                                                           15    9605
XP_006719787                                                           15    9605
XP_005269838                                                           15    9605
XP_005269835                                                           15    9605
XP_005269837                                                           15    9605
XP_006716252                                                           15    9605
XP_006716251                                                           15    9605
XP_006715737                                                           15    9605
XP_006711504                                                           15    9605
XP_006710027                                                           15    9605
>NP_001135993
          Length = 611

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NRCSTSLL 8
          N CSTSL+
Sbjct: 34 NNCSTSLM 41
>NP_001135991
          Length = 611

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NRCSTSLL 8
          N CSTSL+
Sbjct: 34 NNCSTSLM 41
>NP_071357
          Length = 611

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NRCSTSLL 8
          N CSTSL+
Sbjct: 34 NNCSTSLM 41
>NP_942144
          Length = 611

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NRCSTSLL 8
          N CSTSL+
Sbjct: 34 NNCSTSLM 41
>NP_001135992
          Length = 611

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NRCSTSLL 8
          N CSTSL+
Sbjct: 34 NNCSTSLM 41
>NP_004459
          Length = 280

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RCSTSLL 8
           RCSTSL+
Sbjct: 253 RCSTSLV 259
>NP_001230807
          Length = 172

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RCSTSLL 8
           RCSTSL+
Sbjct: 145 RCSTSLV 151
>XP_005270683
          Length = 280

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RCSTSLL 8
           RCSTSL+
Sbjct: 253 RCSTSLV 259
>NP_002221
          Length = 745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RCSTSLL 8
           RC+TS+L
Sbjct: 203 RCTTSIL 209
>NP_000050
          Length = 3418

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    RCSTSLL 8
            RC+TSL+
Sbjct: 3385 RCTTSLI 3391
>NP_068831
          Length = 745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RCSTSLL 8
           RC+TS+L
Sbjct: 203 RCTTSIL 209
>NP_001159906
          Length = 769

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   CSTSLL 8
           CSTSLL
Sbjct: 105 CSTSLL 110
>NP_002206
          Length = 911

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   CSTSLL 8
           CSTSLL
Sbjct: 247 CSTSLL 252
>NP_005240
          Length = 489

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NRCSTSLLA 9
           N CS +LLA
Sbjct: 403 NPCSVNLLA 411
>NP_777576
          Length = 1749

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RCSTSLLA 9
           RCSTS ++
Sbjct: 573 RCSTSFIS 580
>XP_006721941
          Length = 745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RCSTSLL 8
           RC+TS+L
Sbjct: 203 RCTTSIL 209
>XP_006721940
          Length = 745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RCSTSLL 8
           RC+TS+L
Sbjct: 203 RCTTSIL 209
>XP_006721939
          Length = 745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RCSTSLL 8
           RC+TS+L
Sbjct: 203 RCTTSIL 209
>XP_006721938
          Length = 745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RCSTSLL 8
           RC+TS+L
Sbjct: 203 RCTTSIL 209
>XP_006721937
          Length = 745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RCSTSLL 8
           RC+TS+L
Sbjct: 203 RCTTSIL 209
>XP_006721936
          Length = 745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RCSTSLL 8
           RC+TS+L
Sbjct: 203 RCTTSIL 209
>XP_006721935
          Length = 762

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RCSTSLL 8
           RC+TS+L
Sbjct: 220 RCTTSIL 226
>XP_006721934
          Length = 783

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RCSTSLL 8
           RC+TS+L
Sbjct: 241 RCTTSIL 247
>XP_005257370
          Length = 783

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RCSTSLL 8
           RC+TS+L
Sbjct: 241 RCTTSIL 247
>NP_001164409
          Length = 504

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NRCSTSLLA 9
           N C  SLLA
Sbjct: 361 NTCEDSLLA 369
>NP_057452
          Length = 558

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NRCSTSLLA 9
           N C  SLLA
Sbjct: 415 NTCEDSLLA 423
>NP_001240318
          Length = 430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NRCSTSLLA 9
           N C  SLLA
Sbjct: 287 NTCEDSLLA 295
>NP_001269472
          Length = 758

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RCSTSLLA 9
           RC TS+L+
Sbjct: 316 RCRTSILS 323
>NP_000456
          Length = 777

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RCSTSLLA 9
           RC TS+L+
Sbjct: 335 RCRTSILS 342
>XP_006722829
          Length = 753

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NRCSTSLLA 9
           N C  SLLA
Sbjct: 610 NTCEDSLLA 618
>NP_699189
          Length = 576

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RCSTSL 7
           RCSTS+
Sbjct: 310 RCSTSI 315
>NP_001004731
          Length = 362

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  RCSTSLL 8
          RCSTS +
Sbjct: 32 RCSTSFM 38
>NP_001154824
          Length = 1236

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NRCSTSLLA 9
           N CSTS  A
Sbjct: 747 NDCSTSFRA 755
>NP_059138
          Length = 1032

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   CSTSLLA 9
           CSTSL A
Sbjct: 596 CSTSLRA 602
>NP_060664
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CSTSLL 8
           CSTS+L
Sbjct: 434 CSTSML 439
>NP_001164059
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CSTSLL 8
           CSTS+L
Sbjct: 369 CSTSML 374
>NP_001116306
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CSTSLL 8
           CSTS+L
Sbjct: 434 CSTSML 439
>NP_001164035
          Length = 356

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CSTSLL 8
           CSTS+L
Sbjct: 297 CSTSML 302
>NP_001164051
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CSTSLL 8
           CSTS+L
Sbjct: 434 CSTSML 439
>NP_055520
          Length = 1966

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NRCSTS 6
            +RCSTS
Sbjct: 1855 SRCSTS 1860
>NP_149981
          Length = 436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RCSTSLLA 9
           RC TSL A
Sbjct: 320 RCPTSLQA 327
>NP_001164058
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CSTSLL 8
           CSTS+L
Sbjct: 435 CSTSML 440
>NP_001139806
          Length = 1562

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   CSTSLLA 9
           CS SLLA
Sbjct: 234 CSLSLLA 240
>XP_006726609
          Length = 2838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RCSTSL 7
           RC+TSL
Sbjct: 105 RCATSL 110
>XP_005260222
          Length = 316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RCSTSLLA 9
           RC TSL A
Sbjct: 200 RCPTSLQA 207
>XP_006719787
          Length = 2838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RCSTSL 7
           RC+TSL
Sbjct: 105 RCATSL 110
>XP_005269838
          Length = 1236

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NRCSTSLLA 9
           N CSTS  A
Sbjct: 747 NDCSTSFRA 755
>XP_005269835
          Length = 1239

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NRCSTSLLA 9
           N CSTS  A
Sbjct: 751 NDCSTSFRA 759
>XP_005269837
          Length = 1243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   NRCSTSLLA 9
           N CSTS  A
Sbjct: 754 NDCSTSFRA 762
>XP_006716252
          Length = 1937

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NRCSTS 6
            +RCSTS
Sbjct: 1826 SRCSTS 1831
>XP_006716251
          Length = 1975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    NRCSTS 6
            +RCSTS
Sbjct: 1864 SRCSTS 1869
>XP_006715737
          Length = 1349

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   CSTSLLA 9
           CS SLLA
Sbjct: 234 CSLSLLA 240
>XP_006711504
          Length = 527

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   CSTSLL 8
           CSTS+L
Sbjct: 468 CSTSML 473
>XP_006710027
          Length = 2837

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RCSTSL 7
           RC+TSL
Sbjct: 105 RCATSL 110
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.120    0.348 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 558,495
Number of extensions: 443
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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