Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           20     299
NP_004561                                                              20     299
NP_001275703                                                           20     299
XP_005253450                                                           20     299
XP_006719151                                                           20     299
NP_064502                                                              19     869
NP_036558                                                              17    3301
NP_964014                                                              17    3301
NP_004280                                                              17    4312
NP_068749                                                              17    4312
NP_001123553                                                           16    5631
NP_002653                                                              16    5631
XP_005247591                                                           16    5631
XP_005247590                                                           16    5631
NP_950245                                                              16    7355
NP_001243057                                                           16    7355
NP_001243056                                                           16    7355
NP_061028                                                              16    7355
NP_037423                                                              16    7355
XP_006716612                                                           16    7355
XP_006716611                                                           16    7355
NP_001122306                                                           15    9605
NP_001106849                                                           15    9605
NP_060663                                                              15    9605
NP_000524                                                              15    9605
NP_065194                                                              15    9605
NP_955772                                                              15    9605
NP_001258769                                                           15    9605
NP_689614                                                              15    9605
NP_055506                                                              15    9605
NP_001185731                                                           15    9605
NP_001185730                                                           15    9605
NP_149061                                                              15    9605
NP_003751                                                              15    9605
NP_848658                                                              15    9605
NP_001185732                                                           15    9605
XP_005261294                                                           15    9605
XP_005261293                                                           15    9605
XP_006718010                                                           15    9605
XP_006716025                                                           15    9605
XP_006716024                                                           15    9605
XP_005265785                                                           15    9605
NP_003206                                                              15    9605
NP_060299                                                              15    9605
>NP_001275701
          Length = 1486

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NQYLIQVTH 9
            N YLIQVTH
Sbjct: 1260 NLYLIQVTH 1268
>NP_004561
          Length = 1445

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NQYLIQVTH 9
            N YLIQVTH
Sbjct: 1219 NLYLIQVTH 1227
>NP_001275703
          Length = 1264

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NQYLIQVTH 9
            N YLIQVTH
Sbjct: 1038 NLYLIQVTH 1046
>XP_005253450
          Length = 1486

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NQYLIQVTH 9
            N YLIQVTH
Sbjct: 1260 NLYLIQVTH 1268
>XP_006719151
          Length = 1486

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NQYLIQVTH 9
            N YLIQVTH
Sbjct: 1260 NLYLIQVTH 1268
>NP_064502
          Length = 1176

 Score = 18.9 bits (37), Expect =   869
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   NQYLIQVTH 9
           +QYL++VTH
Sbjct: 899 SQYLMEVTH 907
>NP_036558
          Length = 1217

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   NQYLIQVTH 9
           N YL Q+ H
Sbjct: 355 NHYLYQIAH 363
>NP_964014
          Length = 175

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NQYLIQVT 8
           NQYL Q+T
Sbjct: 165 NQYLPQIT 172
>NP_004280
          Length = 694

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   NQYLIQVTH 9
           N Y +Q TH
Sbjct: 660 NHYALQCTH 668
>NP_068749
          Length = 792

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NQYLIQVTH 9
           NQ+L Q TH
Sbjct: 118 NQFLEQETH 126
>NP_001123553
          Length = 1036

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQYLIQVTH 9
           N Y I++TH
Sbjct: 101 NLYTIELTH 109
>NP_002653
          Length = 1074

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQYLIQVTH 9
           N Y I++TH
Sbjct: 101 NLYTIELTH 109
>XP_005247591
          Length = 1036

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQYLIQVTH 9
           N Y I++TH
Sbjct: 101 NLYTIELTH 109
>XP_005247590
          Length = 1074

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQYLIQVTH 9
           N Y I++TH
Sbjct: 101 NLYTIELTH 109
>NP_950245
          Length = 384

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NQYLIQV 7
           NQY IQV
Sbjct: 330 NQYSIQV 336
>NP_001243057
          Length = 335

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NQYLIQV 7
           NQY IQV
Sbjct: 281 NQYSIQV 287
>NP_001243056
          Length = 335

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NQYLIQV 7
           NQY IQV
Sbjct: 281 NQYSIQV 287
>NP_061028
          Length = 632

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NQYLIQV 7
           N YL+QV
Sbjct: 266 NSYLVQV 272
>NP_037423
          Length = 1443

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NQYLIQVT 8
           N+Y++QV+
Sbjct: 615 NRYIVQVS 622
>XP_006716612
          Length = 1365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NQYLIQVT 8
           N+Y++QV+
Sbjct: 537 NRYIVQVS 544
>XP_006716611
          Length = 1440

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NQYLIQVT 8
           N+Y++QV+
Sbjct: 615 NRYIVQVS 622
>NP_001122306
          Length = 277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  QYLIQVTH 9
          +YLI V H
Sbjct: 59 EYLINVIH 66
>NP_001106849
          Length = 1328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QYLIQVT 8
           QY+IQ+T
Sbjct: 172 QYVIQLT 178
>NP_060663
          Length = 1268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QYLIQVT 8
           QY+IQ+T
Sbjct: 172 QYVIQLT 178
>NP_000524
          Length = 277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  QYLIQVTH 9
          +YLI V H
Sbjct: 59 EYLINVIH 66
>NP_065194
          Length = 1819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   QYLIQVTH 9
           +++IQV H
Sbjct: 551 EFMIQVNH 558
>NP_955772
          Length = 242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  QYLIQVTH 9
          +YLI V H
Sbjct: 59 EYLINVIH 66
>NP_001258769
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  QYLIQVTH 9
          +YL Q TH
Sbjct: 17 EYLTQQTH 24
>NP_689614
          Length = 241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NQYLIQVTH 9
           NQY+ +VT+
Sbjct: 98  NQYVNEVTN 106
>NP_055506
          Length = 1485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    YLIQVTH 9
            +LIQ TH
Sbjct: 1371 HLIQTTH 1377
>NP_001185731
          Length = 1591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  NQYLIQVTH 9
          NQ+++ V H
Sbjct: 52 NQHIMMVNH 60
>NP_001185730
          Length = 1621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  NQYLIQVTH 9
          NQ+++ V H
Sbjct: 45 NQHIMMVNH 53
>NP_149061
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQYLIQVTH 9
           +QYL + TH
Sbjct: 326 HQYLQKATH 334
>NP_003751
          Length = 1585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  NQYLIQVTH 9
          NQ+++ V H
Sbjct: 45 NQHIMMVNH 53
>NP_848658
          Length = 920

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   QYLIQVTH 9
           QYL+ + H
Sbjct: 749 QYLLPIVH 756
>NP_001185732
          Length = 515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  NQYLIQVTH 9
          NQ+++ V H
Sbjct: 56 NQHIMMVNH 64
>XP_005261294
          Length = 234

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQYLIQVTH 9
           +QYL + TH
Sbjct: 137 HQYLQKATH 145
>XP_005261293
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQYLIQVTH 9
           +QYL + TH
Sbjct: 165 HQYLQKATH 173
>XP_006718010
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  QYLIQVTH 9
          +YL Q TH
Sbjct: 17 EYLTQQTH 24
>XP_006716025
          Length = 920

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   QYLIQVTH 9
           QYL+ + H
Sbjct: 749 QYLLPIVH 756
>XP_006716024
          Length = 920

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   QYLIQVTH 9
           QYL+ + H
Sbjct: 749 QYLLPIVH 756
>XP_005265785
          Length = 122

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NQYLIQVTH 9
           NQY+ +VT+
Sbjct: 98  NQYVNEVTN 106
>NP_003206
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  NQYLIQVTH 9
          N+Y  QV H
Sbjct: 76 NKYPFQVVH 84
>NP_060299
          Length = 393

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQYLIQVTH 9
           +QYL + TH
Sbjct: 296 HQYLQKATH 304
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.133    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 491,678
Number of extensions: 876
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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