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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              20     390
NP_055481                                                              17    3301
NP_001182485                                                           17    3301
NP_001182484                                                           17    3301
NP_001008781                                                           16    5631
XP_005262121                                                           15    9605
NP_443168                                                              15    9605

>NP_115803
          Length = 1156

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NQSRMGINQ 9
            NQSR GINQ
Sbjct: 1070 NQSRTGINQ 1078




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    NQSRMGINQ 9
            NQS  G+NQ
Sbjct: 1063 NQSGTGLNQ 1071



>NP_055481
          Length = 625

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NQSRMGIN 8
           NQS MG+N
Sbjct: 573 NQSMMGMN 580



>NP_001182485
          Length = 625

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NQSRMGIN 8
           NQS MG+N
Sbjct: 573 NQSMMGMN 580



>NP_001182484
          Length = 643

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NQSRMGIN 8
           NQS MG+N
Sbjct: 591 NQSMMGMN 598



>NP_001008781
          Length = 4557

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  NQSRMGI 7
          +QSRMGI
Sbjct: 64 SQSRMGI 70



>XP_005262121
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RMGINQ 9
           R+GINQ
Sbjct: 153 RLGINQ 158



>NP_443168
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RMGINQ 9
           R+GINQ
Sbjct: 230 RLGINQ 235



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.121    0.319 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 345,545
Number of extensions: 326
Number of successful extensions: 8
Number of sequences better than 10000.0: 7
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)