Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001025226                                                           17    3301
NP_001164                                                              17    3301
NP_060935                                                              17    3301
XP_005267693                                                           17    3301
XP_005267692                                                           17    3301
XP_005251567                                                           17    3301
NP_001238774                                                           17    4312
NP_114119                                                              17    4312
NP_003295                                                              17    4312
XP_006713840                                                           17    4312
XP_006713839                                                           17    4312
XP_005247797                                                           17    4312
XP_005247796                                                           17    4312
XP_005247795                                                           17    4312
NP_006022                                                              16    5631
XP_005261187                                                           16    5631
XP_005261186                                                           16    5631
XP_005261185                                                           16    5631
XP_005261184                                                           16    5631
XP_005261183                                                           16    5631
XP_005261182                                                           16    5631
XP_005261181                                                           16    5631
NP_115750                                                              16    5631
NP_005041                                                              16    7355
NP_002415                                                              16    7355
NP_443104                                                              16    7355
NP_001005180                                                           16    7355
NP_002783                                                              16    7355
NP_653263                                                              16    7355
NP_001020268                                                           16    7355
NP_001020267                                                           16    7355
NP_055336                                                              16    7355
NP_001009562                                                           16    7355
XP_005258256                                                           16    7355
XP_005268003                                                           16    7355
XP_005268006                                                           16    7355
XP_005267999                                                           16    7355
XP_005267997                                                           16    7355
XP_005267998                                                           16    7355
XP_005267996                                                           16    7355
XP_005267995                                                           16    7355
XP_006720286                                                           16    7355
XP_005267993                                                           16    7355
XP_006718904                                                           16    7355
NP_006531                                                              15    9605
NP_848934                                                              15    9605
XP_006721087                                                           15    9605
XP_005253685                                                           15    9605
XP_005253684                                                           15    9605
XP_005251190                                                           15    9605
XP_005251188                                                           15    9605
XP_005251189                                                           15    9605
XP_005251187                                                           15    9605
XP_005251186                                                           15    9605
XP_005251185                                                           15    9605
NP_003950                                                              15    9605
NP_899234                                                              15    9605
NP_777584                                                              15    9605
NP_741997                                                              15    9605
>NP_001025226
          Length = 1502

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NQREFMSKN 9
           NQR+ +SKN
Sbjct: 702 NQRDSISKN 710
>NP_001164
          Length = 1501

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NQREFMSKN 9
           NQR+ +SKN
Sbjct: 702 NQRDSISKN 710
>NP_060935
          Length = 147

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  NQREFMSKN 9
          NQ+EFM  N
Sbjct: 15 NQKEFMLMN 23
>XP_005267693
          Length = 1502

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NQREFMSKN 9
           NQR+ +SKN
Sbjct: 702 NQRDSISKN 710
>XP_005267692
          Length = 1502

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NQREFMSKN 9
           NQR+ +SKN
Sbjct: 702 NQRDSISKN 710
>XP_005251567
          Length = 147

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  NQREFMSKN 9
          NQ+EFM  N
Sbjct: 15 NQKEFMLMN 23
>NP_001238774
          Length = 793

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NQREFMSKN 9
           NQ+EF+S++
Sbjct: 317 NQKEFVSQS 325
>NP_114119
          Length = 886

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  NQREFMSK 8
          N+REF+S+
Sbjct: 92 NEREFISR 99
>NP_003295
          Length = 759

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NQREFMSKN 9
           NQ+EF+S++
Sbjct: 283 NQKEFVSQS 291
>XP_006713840
          Length = 817

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  NQREFMSK 8
          N+REF+S+
Sbjct: 92 NEREFISR 99
>XP_006713839
          Length = 874

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  NQREFMSK 8
          N+REF+S+
Sbjct: 92 NEREFISR 99
>XP_005247797
          Length = 539

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NQREFMSKN 9
           NQ+EF+S++
Sbjct: 317 NQKEFVSQS 325
>XP_005247796
          Length = 661

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NQREFMSKN 9
           NQ+EF+S++
Sbjct: 185 NQKEFVSQS 193
>XP_005247795
          Length = 695

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NQREFMSKN 9
           NQ+EF+S++
Sbjct: 219 NQKEFVSQS 227
>NP_006022
          Length = 3336

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NQREFMSK 8
            NQR+FM +
Sbjct: 1476 NQRQFMDE 1483
>XP_005261187
          Length = 2706

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NQREFMSK 8
            NQR+FM +
Sbjct: 1487 NQRQFMDE 1494
>XP_005261186
          Length = 3139

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NQREFMSK 8
            NQR+FM +
Sbjct: 1358 NQRQFMDE 1365
>XP_005261185
          Length = 3229

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NQREFMSK 8
            NQR+FM +
Sbjct: 1369 NQRQFMDE 1376
>XP_005261184
          Length = 3258

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NQREFMSK 8
            NQR+FM +
Sbjct: 1487 NQRQFMDE 1494
>XP_005261183
          Length = 3268

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NQREFMSK 8
            NQR+FM +
Sbjct: 1487 NQRQFMDE 1494
>XP_005261182
          Length = 3346

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NQREFMSK 8
            NQR+FM +
Sbjct: 1487 NQRQFMDE 1494
>XP_005261181
          Length = 3347

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NQREFMSK 8
            NQR+FM +
Sbjct: 1487 NQRQFMDE 1494
>NP_115750
          Length = 206

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   REFMSKN 9
           +EF+SKN
Sbjct: 157 KEFLSKN 163
>NP_005041
          Length = 606

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   QREFMSK 8
           QRE MSK
Sbjct: 260 QRELMSK 266
>NP_002415
          Length = 467

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NQREFM 6
           NQR+FM
Sbjct: 400 NQRQFM 405
>NP_443104
          Length = 163

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  NQREFMSKN 9
          NQR  MS+N
Sbjct: 65 NQRVSMSRN 73
>NP_001005180
          Length = 324

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   QREFMSKN 9
           QR++ SKN
Sbjct: 174 QRDYCSKN 181
>NP_002783
          Length = 248

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   REFMSKN 9
           REF+ KN
Sbjct: 169 REFLEKN 175
>NP_653263
          Length = 256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   REFMSKN 9
           REF+ KN
Sbjct: 177 REFLEKN 183
>NP_001020268
          Length = 212

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   REFMSKN 9
           REF+ KN
Sbjct: 133 REFLEKN 139
>NP_001020267
          Length = 250

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   REFMSKN 9
           REF+ KN
Sbjct: 171 REFLEKN 177
>NP_055336
          Length = 963

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   QREFMSKN 9
           QR F+ KN
Sbjct: 622 QRRFLKKN 629
>NP_001009562
          Length = 640

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   NQREFMSKN 9
           N+R++++KN
Sbjct: 266 NRRKYITKN 274
>XP_005258256
          Length = 224

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   REFMSKN 9
           REF+ KN
Sbjct: 145 REFLEKN 151
>XP_005268003
          Length = 447

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   QREFMSK 8
           QRE MSK
Sbjct: 101 QRELMSK 107
>XP_005268006
          Length = 447

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   QREFMSK 8
           QRE MSK
Sbjct: 101 QRELMSK 107
>XP_005267999
          Length = 468

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   QREFMSK 8
           QRE MSK
Sbjct: 122 QRELMSK 128
>XP_005267997
          Length = 468

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   QREFMSK 8
           QRE MSK
Sbjct: 122 QRELMSK 128
>XP_005267998
          Length = 468

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   QREFMSK 8
           QRE MSK
Sbjct: 122 QRELMSK 128
>XP_005267996
          Length = 468

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   QREFMSK 8
           QRE MSK
Sbjct: 122 QRELMSK 128
>XP_005267995
          Length = 474

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   QREFMSK 8
           QRE MSK
Sbjct: 260 QRELMSK 266
>XP_006720286
          Length = 519

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   QREFMSK 8
           QRE MSK
Sbjct: 173 QRELMSK 179
>XP_005267993
          Length = 528

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   QREFMSK 8
           QRE MSK
Sbjct: 260 QRELMSK 266
>XP_006718904
          Length = 444

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NQREFM 6
           NQR+FM
Sbjct: 377 NQRQFM 382
>NP_006531
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQREFMSKN 9
           NQ E M+K+
Sbjct: 150 NQEELMNKS 158
>NP_848934
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   REFMSKN 9
           +EFMSK+
Sbjct: 206 KEFMSKH 212
>XP_006721087
          Length = 484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   REFMSKN 9
           R F+SKN
Sbjct: 364 RSFLSKN 370
>XP_005253685
          Length = 1061

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   QREFMSKN 9
           Q+EF +KN
Sbjct: 851 QQEFYAKN 858
>XP_005253684
          Length = 1073

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   QREFMSKN 9
           Q+EF +KN
Sbjct: 863 QQEFYAKN 870
>XP_005251190
          Length = 1395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQREFMSKN 9
           NQ E M+K+
Sbjct: 150 NQEELMNKS 158
>XP_005251188
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQREFMSKN 9
           NQ E M+K+
Sbjct: 150 NQEELMNKS 158
>XP_005251189
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQREFMSKN 9
           NQ E M+K+
Sbjct: 150 NQEELMNKS 158
>XP_005251187
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQREFMSKN 9
           NQ E M+K+
Sbjct: 150 NQEELMNKS 158
>XP_005251186
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQREFMSKN 9
           NQ E M+K+
Sbjct: 150 NQEELMNKS 158
>XP_005251185
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQREFMSKN 9
           NQ E M+K+
Sbjct: 150 NQEELMNKS 158
>NP_003950
          Length = 1068

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   QREFMSKN 9
           Q+EF +KN
Sbjct: 858 QQEFYAKN 865
>NP_899234
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   NQREFMS 7
           N RE+MS
Sbjct: 131 NSREYMS 137
>NP_777584
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   REFMSKN 9
           R F+SKN
Sbjct: 364 RSFLSKN 370
>NP_741997
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  EFMSKN 9
          +FMSKN
Sbjct: 85 DFMSKN 90
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.118    0.307 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 461,778
Number of extensions: 920
Number of successful extensions: 59
Number of sequences better than 10000.0: 59
Number of HSP's gapped: 59
Number of HSP's successfully gapped: 59
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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