Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              20     509
XP_006716142                                                           20     509
XP_005250088                                                           20     509
XP_006716141                                                           20     509
XP_005250085                                                           20     509
XP_006716140                                                           20     509
XP_005250084                                                           20     509
XP_005250083                                                           20     509
XP_005250082                                                           20     509
NP_037385                                                              16    7355
NP_001018067                                                           16    7355
NP_056965                                                              16    7355
NP_851607                                                              16    7355
NP_065156                                                              16    7355
XP_006718243                                                           16    7355
XP_006718242                                                           16    7355
XP_006718241                                                           16    7355
XP_006718240                                                           16    7355
XP_006711530                                                           16    7355
XP_006711529                                                           16    7355
XP_006711528                                                           16    7355
NP_001268357                                                           15    9605
NP_036276                                                              15    9605
NP_001268675                                                           15    9605
NP_055160                                                              15    9605
XP_006716821                                                           15    9605
XP_006716820                                                           15    9605
XP_006716819                                                           15    9605
XP_006716818                                                           15    9605
NP_001025052                                                           15    9605
>NP_733751
          Length = 4911

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NQMVFSEQA 9
           +QMVFSEQA
Sbjct: 553 DQMVFSEQA 561
>XP_006716142
          Length = 4927

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NQMVFSEQA 9
           +QMVFSEQA
Sbjct: 554 DQMVFSEQA 562
>XP_005250088
          Length = 4928

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NQMVFSEQA 9
           +QMVFSEQA
Sbjct: 554 DQMVFSEQA 562
>XP_006716141
          Length = 4959

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NQMVFSEQA 9
           +QMVFSEQA
Sbjct: 554 DQMVFSEQA 562
>XP_005250085
          Length = 4979

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NQMVFSEQA 9
           +QMVFSEQA
Sbjct: 554 DQMVFSEQA 562
>XP_006716140
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NQMVFSEQA 9
           +QMVFSEQA
Sbjct: 554 DQMVFSEQA 562
>XP_005250084
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NQMVFSEQA 9
           +QMVFSEQA
Sbjct: 554 DQMVFSEQA 562
>XP_005250083
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NQMVFSEQA 9
           +QMVFSEQA
Sbjct: 553 DQMVFSEQA 561
>XP_005250082
          Length = 4983

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NQMVFSEQA 9
           +QMVFSEQA
Sbjct: 554 DQMVFSEQA 562
>NP_037385
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   QMVFSE 7
           QMVFSE
Sbjct: 125 QMVFSE 130
>NP_001018067
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   QMVFSE 7
           QMVFSE
Sbjct: 125 QMVFSE 130
>NP_056965
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   QMVFSE 7
           QMVFSE
Sbjct: 125 QMVFSE 130
>NP_851607
          Length = 742

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NQMVFSE 7
           NQ+VF+E
Sbjct: 296 NQLVFAE 302
>NP_065156
          Length = 688

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NQMVFSE 7
           NQ+VF+E
Sbjct: 296 NQLVFAE 302
>XP_006718243
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   QMVFSE 7
           QMVFSE
Sbjct: 188 QMVFSE 193
>XP_006718242
          Length = 258

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   QMVFSE 7
           QMVFSE
Sbjct: 188 QMVFSE 193
>XP_006718241
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   QMVFSE 7
           QMVFSE
Sbjct: 125 QMVFSE 130
>XP_006718240
          Length = 413

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   QMVFSE 7
           QMVFSE
Sbjct: 188 QMVFSE 193
>XP_006711530
          Length = 469

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NQMVFSE 7
           NQ+VF+E
Sbjct: 296 NQLVFAE 302
>XP_006711529
          Length = 546

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NQMVFSE 7
           NQ+VF+E
Sbjct: 154 NQLVFAE 160
>XP_006711528
          Length = 742

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NQMVFSE 7
           NQ+VF+E
Sbjct: 296 NQLVFAE 302
>NP_001268357
          Length = 703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NQMVFSEQA 9
           NQ  FSE+A
Sbjct: 194 NQFNFSERA 202
>NP_036276
          Length = 699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NQMVFSEQA 9
           NQ  FSE+A
Sbjct: 190 NQFNFSERA 198
>NP_001268675
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NQMVFSEQA 9
           N  +FSEQA
Sbjct: 888 NSDLFSEQA 896
>NP_055160
          Length = 2279

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    VFSEQA 9
            VFSEQA
Sbjct: 1545 VFSEQA 1550
>XP_006716821
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  NQMVFSEQA 9
          NQ  FSE+A
Sbjct: 13 NQFNFSERA 21
>XP_006716820
          Length = 673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NQMVFSEQA 9
           NQ  FSE+A
Sbjct: 225 NQFNFSERA 233
>XP_006716819
          Length = 730

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NQMVFSEQA 9
           NQ  FSE+A
Sbjct: 221 NQFNFSERA 229
>XP_006716818
          Length = 734

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NQMVFSEQA 9
           NQ  FSE+A
Sbjct: 225 NQFNFSERA 233
>NP_001025052
          Length = 185

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  VFSEQA 9
          VFSEQA
Sbjct: 91 VFSEQA 96
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.115    0.282 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 393,323
Number of extensions: 609
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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