Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              20     509
NP_005913                                                              20     509
XP_005267046                                                           20     509
XP_005267045                                                           20     509
NP_001243779                                                           16    7355
NP_001230271                                                           16    7355
NP_596869                                                              16    7355
NP_597709                                                              16    7355
NP_064715                                                              16    7355
XP_006714215                                                           16    7355
XP_006714214                                                           16    7355
XP_005262915                                                           16    7355
XP_006712787                                                           16    7355
XP_006712786                                                           16    7355
NP_055806                                                              16    7355
NP_001254479                                                           16    7355
NP_001159845                                                           15    9605
NP_001103132                                                           15    9605
XP_006716564                                                           15    9605
XP_005250826                                                           15    9605
XP_006714311                                                           15    9605
XP_006714310                                                           15    9605
NP_001106                                                              15    9605
>NP_006715
          Length = 1558

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NNVGRSASR 9
           NNVGR ASR
Sbjct: 102 NNVGRPASR 110
>NP_005913
          Length = 1608

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NNVGRSASR 9
           NNVGR ASR
Sbjct: 102 NNVGRPASR 110
>XP_005267046
          Length = 1554

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NNVGRSASR 9
           NNVGR ASR
Sbjct: 102 NNVGRPASR 110
>XP_005267045
          Length = 1604

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NNVGRSASR 9
           NNVGR ASR
Sbjct: 102 NNVGRPASR 110
>NP_001243779
          Length = 34350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    NNVGRSA 7
            NNVG+S+
Sbjct: 6707 NNVGKSS 6713

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    NNVGRSASR 9
            N+VGRS+ +
Sbjct: 5582 NDVGRSSCK 5590
>NP_001230271
          Length = 1030

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    NNVGRSASR 9
            NN GR+ +R
Sbjct: 1002 NNTGRAQAR 1010
>NP_596869
          Length = 33423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    NNVGRSA 7
            NNVG+S+
Sbjct: 5780 NNVGKSS 5786

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    NNVGRSASR 9
            N+VGRS+ +
Sbjct: 4655 NDVGRSSCK 4663
>NP_597709
          Length = 756

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NNVGRSAS 8
           NN+G SAS
Sbjct: 352 NNLGPSAS 359
>NP_064715
          Length = 441

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   NNVGRSASR 9
           N+VGR+A++
Sbjct: 110 NSVGRTAAQ 118
>XP_006714215
          Length = 3544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    NVGRSA 7
            NVGRSA
Sbjct: 1750 NVGRSA 1755
>XP_006714214
          Length = 3544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    NVGRSA 7
            NVGRSA
Sbjct: 1750 NVGRSA 1755
>XP_005262915
          Length = 3544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    NVGRSA 7
            NVGRSA
Sbjct: 1750 NVGRSA 1755
>XP_006712787
          Length = 33978

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    NNVGRSA 7
            NNVG+S+
Sbjct: 6708 NNVGKSS 6714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    NNVGRSASR 9
            N+VGRS+ +
Sbjct: 5583 NDVGRSSCK 5591
>XP_006712786
          Length = 35484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    NNVGRSA 7
            NNVG+S+
Sbjct: 6708 NNVGKSS 6714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    NNVGRSASR 9
            N+VGRS+ +
Sbjct: 5583 NDVGRSSCK 5591
>NP_055806
          Length = 3526

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    NVGRSA 7
            NVGRSA
Sbjct: 1732 NVGRSA 1737
>NP_001254479
          Length = 35991

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    NNVGRSA 7
            NNVG+S+
Sbjct: 7024 NNVGKSS 7030

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    NNVGRSASR 9
            N+VGRS+ +
Sbjct: 5899 NDVGRSSCK 5907
>NP_001159845
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  GRSASR 9
          GRSASR
Sbjct: 43 GRSASR 48
>NP_001103132
          Length = 4284

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NNVGRSASR 9
           N+VGR  S+
Sbjct: 180 NSVGRGVSK 188
>XP_006716564
          Length = 1185

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  GRSASR 9
          GRSASR
Sbjct: 27 GRSASR 32
>XP_005250826
          Length = 1221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  GRSASR 9
          GRSASR
Sbjct: 27 GRSASR 32
>XP_006714311
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  GRSASR 9
          GRSASR
Sbjct: 43 GRSASR 48
>XP_006714310
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  GRSASR 9
          GRSASR
Sbjct: 43 GRSASR 48
>NP_001106
          Length = 1251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  GRSASR 9
          GRSASR
Sbjct: 27 GRSASR 32
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.114    0.294 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 360,787
Number of extensions: 610
Number of successful extensions: 28
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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