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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001138389                                                           18    1135
NP_001138386                                                           18    1135
NP_000132                                                              18    1135
NP_075259                                                              18    1135
NP_001138385                                                           18    1135
XP_006717776                                                           18    1135
XP_006717773                                                           18    1135
XP_006717772                                                           18    1135
XP_006717771                                                           18    1135
NP_004516                                                              16    5631
XP_006712591                                                           16    5631
XP_006712590                                                           16    5631
XP_006712589                                                           16    5631

>NP_001138389
          Length = 705

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  NNRTVLIVE 9
          NNRTVLI E
Sbjct: 82 NNRTVLIGE 90



>NP_001138386
          Length = 709

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  NNRTVLIVE 9
          NNRTVLI E
Sbjct: 82 NNRTVLIGE 90



>NP_000132
          Length = 821

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  NNRTVLIVE 9
          NNRTVLI E
Sbjct: 82 NNRTVLIGE 90



>NP_075259
          Length = 822

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  NNRTVLIVE 9
          NNRTVLI E
Sbjct: 82 NNRTVLIGE 90



>NP_001138385
          Length = 769

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  NNRTVLIVE 9
          NNRTVLI E
Sbjct: 82 NNRTVLIGE 90



>XP_006717776
          Length = 806

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NNRTVLIVE 9
           NNRTVLI E
Sbjct: 101 NNRTVLIGE 109



>XP_006717773
          Length = 807

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NNRTVLIVE 9
           NNRTVLI E
Sbjct: 101 NNRTVLIGE 109



>XP_006717772
          Length = 838

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NNRTVLIVE 9
           NNRTVLI E
Sbjct: 101 NNRTVLIGE 109



>XP_006717771
          Length = 839

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NNRTVLIVE 9
           NNRTVLI E
Sbjct: 101 NNRTVLIGE 109



>NP_004516
          Length = 4655

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NRTVLIVE 9
            NRTVL+ E
Sbjct: 2224 NRTVLVSE 2231



>XP_006712591
          Length = 3308

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NRTVLIVE 9
            NRTVL+ E
Sbjct: 2229 NRTVLVSE 2236



>XP_006712590
          Length = 3897

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NRTVLIVE 9
            NRTVL+ E
Sbjct: 1466 NRTVLVSE 1473



>XP_006712589
          Length = 4660

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NRTVLIVE 9
            NRTVL+ E
Sbjct: 2229 NRTVLVSE 2236



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.135    0.337 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 379,790
Number of extensions: 573
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)