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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001243398                                                           19     869
NP_036462                                                              19     869
NP_001243397                                                           19     869
XP_005269721                                                           19     869
NP_001092871                                                           16    5631
NP_001092870                                                           16    5631
NP_003910                                                              16    5631
NP_001092882                                                           16    5631
NP_006757                                                              16    5631
NP_001092883                                                           16    5631
NP_004766                                                              16    5631
XP_006722642                                                           16    5631
XP_006722641                                                           16    5631
XP_005258444                                                           16    5631
XP_005273706                                                           16    5631
XP_006716230                                                           16    5631
XP_005250736                                                           16    5631
XP_006716229                                                           16    5631
XP_006716228                                                           16    5631
XP_005250734                                                           16    5631
XP_005250732                                                           16    5631
NP_115543                                                              16    7355
XP_005255740                                                           16    7355
XP_006721041                                                           16    7355
XP_006721040                                                           16    7355
XP_005255739                                                           16    7355

>NP_001243398
          Length = 1781

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NMSVNLMSA 9
            NMSVNLM A
Sbjct: 1674 NMSVNLMPA 1682



>NP_036462
          Length = 2073

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NMSVNLMSA 9
            NMSVNLM A
Sbjct: 1966 NMSVNLMPA 1974



>NP_001243397
          Length = 1890

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NMSVNLMSA 9
            NMSVNLM A
Sbjct: 1783 NMSVNLMPA 1791



>XP_005269721
          Length = 2073

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    NMSVNLMSA 9
            NMSVNLM A
Sbjct: 1966 NMSVNLMPA 1974



>NP_001092871
          Length = 462

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NMSVNLMSA 9
           N+ +N+MSA
Sbjct: 145 NLIINIMSA 153



>NP_001092870
          Length = 451

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NMSVNLMSA 9
           N+ +N+MSA
Sbjct: 145 NLIINIMSA 153



>NP_003910
          Length = 437

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NMSVNLMSA 9
           N+ +N+MSA
Sbjct: 145 NLIINIMSA 153



>NP_001092882
          Length = 2004

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    NMSVNLM 7
            NM VNLM
Sbjct: 1898 NMGVNLM 1904



>NP_006757
          Length = 2004

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    NMSVNLM 7
            NM VNLM
Sbjct: 1898 NMGVNLM 1904



>NP_001092883
          Length = 2004

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    NMSVNLM 7
            NM VNLM
Sbjct: 1898 NMGVNLM 1904



>NP_004766
          Length = 382

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NMSVNLM 7
           N+SVNLM
Sbjct: 367 NISVNLM 373



>XP_006722642
          Length = 338

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NMSVNLM 7
           N+SVNLM
Sbjct: 323 NISVNLM 329



>XP_006722641
          Length = 344

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NMSVNLM 7
           N+SVNLM
Sbjct: 329 NISVNLM 335



>XP_005258444
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   NMSVNLM 7
           N+SVNLM
Sbjct: 355 NISVNLM 361



>XP_005273706
          Length = 1997

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    NMSVNLM 7
            NM VNLM
Sbjct: 1891 NMGVNLM 1897



>XP_006716230
          Length = 346

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NMSVNLMSA 9
          N+ +N+MSA
Sbjct: 54 NLIINIMSA 62



>XP_005250736
          Length = 396

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NMSVNLMSA 9
           N+ +N+MSA
Sbjct: 104 NLIINIMSA 112



>XP_006716229
          Length = 408

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NMSVNLMSA 9
           N+ +N+MSA
Sbjct: 116 NLIINIMSA 124



>XP_006716228
          Length = 428

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NMSVNLMSA 9
           N+ +N+MSA
Sbjct: 145 NLIINIMSA 153



>XP_005250734
          Length = 464

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NMSVNLMSA 9
           N+ +N+MSA
Sbjct: 181 NLIINIMSA 189



>XP_005250732
          Length = 473

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NMSVNLMSA 9
           N+ +N+MSA
Sbjct: 181 NLIINIMSA 189



>NP_115543
          Length = 813

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SVNLMSA 9
           SVN+MSA
Sbjct: 300 SVNIMSA 306



>XP_005255740
          Length = 551

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SVNLMSA 9
           SVN+MSA
Sbjct: 310 SVNIMSA 316



>XP_006721041
          Length = 680

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SVNLMSA 9
           SVN+MSA
Sbjct: 167 SVNIMSA 173



>XP_006721040
          Length = 661

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SVNLMSA 9
           SVN+MSA
Sbjct: 148 SVNIMSA 154



>XP_005255739
          Length = 823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SVNLMSA 9
           SVN+MSA
Sbjct: 310 SVNIMSA 316



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.109    0.254 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 221,084
Number of extensions: 179
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)